This page counts for the book, as well as its film adaptations.
- Sorrowful and Immaculate Hearts: In "Anti-Social", Bruce inadvertantly acquires a rat after being dumped in a dumpster by Poison Ivy. He names it Templeton.
- Barnyard: When The Farmer gets knocked out, Otis gives him a copy of the Charlotte's Web book to make it look like the Farmer fell asleep reading it.
- Leo: Near the beginning of the movie, Mrs. Salinas is starting her yearly reading of Charlotte's Web. Squirtle bemoans the book because it always gets the kids teary-eyed, and crying is for weaklings (with Leo agreeing). When Ms. Malkin replaces her, one of the first things she does is suck up the book in her portable vacuum. At the end of the year, Ms. Malkin ejects the book from the vacuum and finishes it; Leo and Squirtle also get tearful at the ending, Squirtle loudest of everyone in the class (with snot bubbles coming out of his nose).
- Sonic the Hedgehog (2020): The book is referenced to emphasize Dr. Robotnik's Lack of Empathy nature: in his rambling assault against Major Bennington, the doctor asks Bennington if he ever read Charlotte's Web, saying that the book ends with the death of the titular spider after she lays a "big creepy egg sac". He then compares Charlotte's egg sac to his ovular white drones.
- The main character of See You At Harry's is named Fern after the protagonist of this book.
- Emma from Because of the Rabbit longs for a best friend and hopes to be half of an "and," like Charlotte and Wilbur.
- In Diary of a Wimpy Kid: Dog Days, Greg is given the book to read by his mom. Aware of the Death by Newbery Medal trope, he predicts that either Wilbur or Fern will die at the end (he doesn't find out it's Charlotte because he doesn't read the book all the way through).
- In The Getaway, Greg mentions that he's afraid of spiders when encountering a tarantula. He mentions Charlotte's Web if it had been a very short book. Showing a picture of Greg as a farmhand killing Charlotte with a spray can.
- In the Time Warp Trio book Summer Reading is Killing Me, Charlotte makes an appearance to help the kids figure out where to go.
- The title character of Melissa is a fourth grade trans girl whose school is hosting a play of the novel. Melissa wants to play Charlotte, but the school won't even consider her for a female role because she was assigned male at birth and the story starts with her still presenting as male.
- In Small as an Elephant, Jack is delighted to see that the barn he's going to spend the night in has a loft, because ever since his class read Charlotte's Web in third grade, he's wanted to look down from a loft.
- Mermaids of Eriana Kwai: When Meela was a kid, she named the spider that lived in her window Charlotte after the character from the book, which her mama read to her.
- In Lily and Dunkin, Lily calls Meatball "Some dog" in a reference to "Some pig."
- Frasier: Charlotte (Laura Linney) names her matchmaking business after the novel.
- Hannah Montana: In "Everybody Was Best Friend Fighting", Hannah plays on a celebrity tennis match against Dakota Fanning, and her partner says they're gonna make her cry harder than she did in "that pig movie".
- iCarly: In "iGot Detention", Sam mentions a book titled Scarlett's Web.
- Early in Season 2 of The Marvelous Mrs. Maisel, Susie stays in the Weissman's apartment while they are in France and her apartment is getting fumigated. One scene during her Boredom Montage is her bawling her eyes out while reading Charlotte's Web.
- A Kevin & Kell strip has Wilbur running an ad agency ... until Charlotte quits.
- The Nostalgia Critic:
- Charlotte's death is listed #4 on his list of "Top 11 Saddest Nostalgic Moments".
- In his review of Gordy, the Critic brings up the 1973 animated film when he points out that a story about a pig on a farm who's separated from his family but somehow becomes world famous is nothing new.
- In his review of Stuart Little, when Stuart's (fake) father tells Stuart not to let the bedbugs bite, the Critic gives us this line:Critic (as Stuart's Father): The spider named Charlotte smells nice, but she will eat you.
- SuperMarioLogan: In "Who Wants to Be a Millionaire?: Episode 2", Brooklyn T. Guy asks Toad this question:In the children's book, Charlotte's Web, what sort of animal was Charlotte?
A. Pig
B. Spider
C. Sheep
D. Honey Bee
- Drawn Together: The episode "Charlotte's Web of Lies" involves Spanky having a one-night stand with Charlotte.
- Family Guy: In "Mother Tucker", Lois mentions that Charlotte had to overcome Tourette's; in the ensuing cutaway, we see Fern noticing a spider web with "WHORE" written in it.
- Futurama: In "Teenage Mutant Leela's Hurdles", one of the stories in the book A Child's Garden of Space Legends is called "Charlotte's Tholian Web".
- Martha Speaks: In a flashback scene in "The Martha Code", Alice reads a book about a pig, and just as she about to reach the end, her older brother Ronald spoils it by saying, "The spider dies."
- Robot Chicken:
- A sketch from "Rabbits on a Roller Coaster" depicts Charlotte writing "$4.99/lb" on her web.
- A sketch from "May Cause Season 11 to End" features a man watching "Charlotte's Webcam" on his computer and masturbating to it.
- The Simpsons:
- In "Lisa's Substitute", Mr. Bergstrom reads Charlotte's Web to Lisa's class.
- In "Treehouse of Horror XIII", Bart, after being turned into a spider, weaves a web reading "EAT MY SHORTS".
- In "Mobile Homer", Homer tries to get spiders off his face by spraying with a poison spray called "Charlotte's Dead".
