Villains from the 1993 Series
Lawrence Limburger, otherwise known as "Boss", "Mister Limboiger" and "Stinkface", is a well-spoken denizen of destruction and disaster. He is the epitomy of greed, with a hunger for power that would outdo most dictators. His most fervent wish is to become the High Poobah of not only Earth, but Plutark as well, and if it weren't for those stupid gerbils on bikes he'd have his wish by now. It's not for lack of trying that he's still a much-maligned minnow in the pool of power- it's that through a combination of incompetent goons, bad luck and not crossing one T that has him pulling his fins in frustration.
The 2006 series has the Catatonians and Ronaldo Rump replace him as the main antagonists, but he eventually makes an appearance where it is revealed that he was banished to Earth after the Plutarkians were defeated and that he and Karbunkle had become lackeys to the Pit Boss all this time. He ends up suffering another humiliating defeat at the Biker Mice's hands, but he does get one more chance to get even with the mice near the end of the series.
- Added Alliterative Appeal: Lends a lovely layer to Limburger's loquacious lambasting of his lackeys.
- Alliterative Name: His first, middle, and last names all begin with the letter L.
- Antagonist in Mourning: Subverted in the funniest way possible: after The Pulverizer reports the death of the Biker Mice in "Steal of The Century", he quietly asks for a moment and walks into a closetā¦ and then jumps up and down while shouting "Yes, yes, yes, yesā¦".
- Bad Boss: Limburger has no respect for his underlings—consequently, they only serve him because he's rich.
- Big Bad: He is the main antagonist of the original 1993 series, always trying to stop the Biker Mice from preventing his schemes to harvest Earth's resources.
- Big Bad Wannabe: He's demoted to this in the 2006 revival, as while he is still a threat to the Biker Mice, he appears in only a few episodes and his plans to overthrow the Pit Boss and upstage Ronaldo Rump and the Catatonians fail miserably. Tellingly, the Biker Mice aren't even the ones who deal out his final defeat. Instead Ronaldo and the Catatonians use him and Brie as pawns to bring them both Stoker and two regenerators, then dispose of them both afterwards by turning them into fish.
- Big "NO!": Happens everytime his building is destroyed in some shape or form - which means this happens a lot.
- Card-Carrying Villain: Openly describes himself as being evil and despicable. He put it best in "Hard Rock" when he was called out on not holding his part of an agreement after giving his word.Limburger: If my word was worth anything, I wouldn't be much of a villain, now would I?
- Collector of the Strange: He owns a personal museum containing the last living kind of the species he wiped out on the alien planets Plutark conquered.
- Corrupt Corporate Executive: He is the CEO of Limburger Corporation, although by the looks of things, he's a low-level manager in the grand scheme of the Plutarkian society (perhaps because Earth's really not that important). He still has quotas to fill and expectations to meet, however. Wants to buy and then strip-mine the entire planet (except Detroit). He doesn't seem to be limited to just buying up real estate however—he sometimes will just waltz in and take the generic resources he wants.
- The MegaCorp gives him the backing to do so. Offworld resources are barely if ever used.
- Deadpan Snarker: Frequently makes cutting remarks about his minions' incompetence as well as his disdain for them.
- Demoted to Extra: The Catatonians and Ronaldo Rump replace him as the main villain in the 2006 revival. As a result, he only appears in a handful of episodes and fails miserably in his attempts to upstage the Catatonians.
- Determinator: Limburger is very determined to get rid of the Biker Mice and he has no intention of giving up.
- Dude, Where's My Respect?: Despite his ego and supposed intellect, multiple episodes demonstrate he's not very respected among other Plutarkians. Lord Camembert makes it clear on different occasions that he feels Napoleon Brie is more deserving of respect, and was outraged to see Limburger hosting a meeting for Plutarkian bosses when Brie was supposed to do it (Limburger temporarily poisoned him).
- Embarrassing Nickname: "Drippy Drawers", due to a problem he had as a kid. His big sister Latoya still calls him that, an habit she passed to her son Marshal.
- Enemy Mine: He had to ally himself with the Biker Mice in "Cheese Cadets", after The Pulverizer rebelled against him.
- Even Bad Men Love Their Parents:
- His speech in "Virtual Unreality" included thanks to his parents for how they raised him.
- In "High-Rolling Rodents" he fondly remembered his mom's words of advice twice.
- Even Evil Has Standards:
- Limburger is put off by Brie's crassness and Grease Pit's, well, greasiness.
- He's embarrassed by the infamous traditional greeting of his people, for good reasons.
- Subverted with the Loogie Brothers. They are two Plutarkians so smelly that even other Plutarkians (infamous for their smell of fish) can't take them... So he grabbed a gas mask and made them stink even worse to try and get the people of Chicago to run away.
- His nephew Marshall is such an obnoxious and troublesome Bratty Half-Pint that even he is scared of him.
- Evil Laugh: Limburger has an especially ominous laugh.
- Evil Power Vacuum: When Limburger is accidentally teleported away from Earth in "A scent, a memory, a far distant Cheese" , Greasepit tries to act like a Dragon Ascendant to the Monster of the Week, a duo of size changing mini Kaiju, Gorgonzola and Munsterella, who subsequently laugh him off and take over Liumburger Enterprises.
- Evil Versus Evil: He isn't just busy fighting the Biker Mice, but also his old rival and fellow Plutarkian Brie; each of them wish to conquer the other's city. His few appearances in the 2006 series also make it clear that he has beef with the Pit Boss, Ronaldo Rump and the Catatonians. He attempts to upstage them, coming very close to succeeding in overthrowing the Pit Boss but ultimately failing, and failing miserably against the latter two to the point that they're the ones who take him and Brie out for good in the Grand Finale, instead of the Biker Mice.
- Fat Bastard: He is rather obese and is not a nice person at all.
- Faux Affably Evil: He acts polite and sophisticated, but he still had a hand in bringing Mars to near devastation and shows absolutely no guilt whatsoever for all the lives he's ruined and the planets he's destroyed by stealing resources for Plutark.
- Fish People: Like all Plutarkians, he is a fish alien.
- Forced Transformation: In the 2006 revival, his final fate is to be turned into a non-anthropomorphic fish while still capable of speech.
- Goofy Print Underwear: He's sometimes shown to wear red boxers with hunks of swiss cheese printed on them.
- Graceful Loser: Subverted: he pretended to accept his defeat at the hands of Brie in "Back to Mars", but he was actually acting to drive a wedge between Brie and Number One by using the latter's Berserk Button. In fact, he is usually everything but it: at best he can be too depressed to react.
- Kick the Dog: There are several occasions where he does something underhanded and cruel just to prove he can.
- He pours a jug of hot chocolate onto the heads of some carolers for no apparent reason in the Christmas Episode "Chill Zone".
- In "Pwetty Wady", when Karbunkle informs him that the building he plans to blow up is an orphanage, he gloats that this means there won't be any parents complaining about who gets hurt in the process.
- Klingons Love Shakespeare: Even on Mars he was a big fan of the gangster period, which explains his choice of disguise.
- Large and in Charge: He is very big and calls the shots.
- Also, he actually obtains the title of High Chairman twice. Sure, he lose it both times by the end of the episode, but still...
- Latex Perfection: Like all Plutarkians stationed on Earth, he wears a realistic mask to appear human. In fact, he wears it so often that one is likely to forget that he's really a fish alien disguised as a human.
- Leitmotif: He has his own theme music, which often plays during scenes at Limburger Plaza.
- Magnificent Bastard: He tends to fluctuate between this and Smug Snake: while it is not seen too often, he is good at manipulating others and finding a way to assure his survival even in the worst situation. That's not even considering how good he has become in anticipating the Biker Mice's moves. For some reasons, he seems to be at his best when on Mars.
- Non-Action Big Bad: He never refrains from grabbing a weapon to try and blast the mice, but he doesn't appears to be stronger than a regular human nor he know how to fight, so he has to rely on his minions and his cunning
- Riches to Rags: In "Stone Broke", Camembert cut his funding, forcing him to sell the tower and live in a trailer.
- Sesquipedalian Loquaciousness: Quite a lot of his dialogue consists of delivering wordy insults to the Biker Mice, his minions, or anyone else that has severely irked him.
- Suddenly Shouting: Sometimes raises his voice without warning, especially when he's chewing out his goons for failing him.
- Surrounded by Idiots: A major source of humour is his frustration at having to put up with the ineptitude of his underlings. It even gets Lampshaded by the Biker Mice in the very first episode...
- The Starscream: Limburger does not like taking orders from other people, so generally he will plot to overthrow his superiors such as Lord Camembert and Dominic T. Stilton, or The Pit Boss and Ronaldo Rump in the 2006 series.
- Unsportsmanlike Gloating: After winning the title of governor of Earth in "Diet of Worms", he pretty cheerfuly calls the Others Plutarkian bosses losers and reminds them their future is going to suck compared to his.
- "The Villain Sucks" Song: "Look out Below" on the album by Jeff Scott Soto appears to be about Limburger.
- Villain Team-Up: With the Pit Boss in "Pitfall": he gave the Pit Boss men and access to his teleporter in exchange to help in capturing Provoloni, who was hiding in Four-By's territory.
- Villain with Good Publicity: He is seen by most of Chicago as a stand-up citizen and a humanitarian in spite of really using his business as a cover for stealing resources from Earth for Plutark.
- Who's Laughing Now?: His relationship with Karbunkle: back on Mars they both worked for Stilton, but as his personal scientist Karbunkle was in a better position, and boy did he take advantage of it: he constantly made fun of Limburger, played pranks on him and insulted him in front of their boss. Now that Limburger is in charge he never misses an occasion for picking on Karbunkle and getting angry at him for failing. That said, he still treats him better than Greasepit.
- You Fool!: Yells that the Biker Mice are fools in "Back to Mars" when it looks like the four of them will perish while the teleporter explodes.Limburger: You fools! You've doomed us all!
Karbunkle is a god-only-knows-what from nobody-knows-where, and he's almost completely nuts to boot. Word of God has it that Karbunkle's origins may be rooted in World War II, perhaps as a German scientist gone mad. As noted, Karbunkle experimented on the Biker Mice when they were captured, and he's responsible for their Cursed with Awesome cybernetic implants.
He returns in the revival, again serving Limburger, though he much later ends up assisting Ronaldo Rump and the Catatonians.
- Ambiguously Human: He doesn't look any different from a normal human being other than his large head, the black tube connected to it and the fact he only has four fingers while the other humans have five, but he's shown on Mars with Limburger and another Plutarkian named Dominic T. Stilton in the flashbacks of "Once Upon a Time on Mars". This is lampshaded in the 2006 episode "Once Upon a Time on Earth" when Dr. Catorkian asks Karbunkle if he is human and he replies with "Of course I am....I think."
- Combat Stilettos: A very Rare Male Example. His boots have pointed heels.
- Dirty Coward: He usually flees at the first sight of danger, unless he can get his hands on a powerful enough weapon.
- Demoted to Extra: With the Catatonians introduced as the new antagonists of the 2006 revival, Karbunkle and his boss are reduced to becoming minor villains who only appear in a handful of episodes.
- Evil Genius: He is the brains of Limburger's minions.
- Evil Redhead: He has red hair and is definitely evil.
- Four Fingered Hand: Strangely, seeing as every human is drawn with five. It does lend weight at the theory he is a Human Alien.
- For Science!: He loves his experiments.
- Kick the Dog: He was actually glad when Limburger sold Greasepit as a slave to the Sand Riders.
- Forced Transformation: He ends up being turned into a turtle by the end of the 2006 revival.
- Goggles Do Nothing: At least, not that we know of. And yet, he's never seen without them.
- At least he wears them down over his eyes instead of eternally up on his forehead, like most characters.
- Greed: He was perfectly happy to leave Limburger to the Biker Mice until Greasepit reminded him he was the one paying him.
- I Need to Go Iron My Dog: He once fleed from battle shouting: "If you excuse me, I have some lobotomies to perform".
- Laser-Guided Karma: Considering how much of a troll he was to Limburger back on Mars, it's difficult to fell sorry for him when the Plutarkian acts as a Bad Boss to him.
- Lean and Mean: He is very skinny and also completely depraved.
- Mad Scientist: Dr. Karbunkle is distilled batshit insane scientist.
- My Brain Is Big: He's a very intelligent villain with a high forehead.
- Mysterious Past: Nothing is known about where he comes from and why he serves the Plutarkians.
- Necromancer: He invented a liquid called mobility juice in "Motocross Trap" that was capable to bring inanimate objects to life. If used on a corpse, it makes it become a zombie.
- Noodle Incident: "Biker Knights of The Round Table " reveals he was married( multiple times, since he specifically said "first wife") and she was some kind of gorgon.
- Omnidisciplinary Scientist: Chemistry, engineering, robotic, biologyā¦ there isn't a branch of science he hasn't conqueredā¦ and defiled.
- Overly Long Name: The Clip Show episode "Mad Scientist Wanted" reveals that his full name is Benjamin Boris Zachary Karbunkle.
- Rewarded as a Traitor Deserves:In an attempt to save himself when Limburger and Brie's plans fail and they are captured by the Catatonians, he betrays Limburgerā and proceeds to help Rump and the Catatonians with fixing the Regenerator's. Dr. Catorkian, who had been getting increasingly fed up with Karbunkle, ends up using the regenerator to transform him into a turtle once he's no longer needed.
- Villainous Crossdresser: The aforementioned Combat Stilettos.
- Why Did It Have to Be Snakes?: He is afraid of, or at least disgusted by, frogs.
Limburger's rather dim-witted henchman who drips grease everywhere (hence the name). Limburger found him while searching for some big muscle to help him take over Mars... needless to say, Greasepit's resume was a little beefed up.
- Achievements in Ignorance: He is the one who caused the wounds that almost killed the Biker Mice and allowed Karbunkle to capture and experiment on themā¦ despite hitting them by dumb luck with a weapon he had no idea how to use.
- Ambiguously Human: He may appear to be your standard human thug at first glance, but "Once Upon a Time on Mars" reveals that the first time Limburger hired him was on Mars, which suggests that he may be a Human Alien. Which would certainly explain the whole "constantly sweating oil" thing.
- Badass Biker: Averted: he tries, but he doesn't have a grain of the skills of the Biker Mice.
- Bald of Evil: He has no hair.
- Butt-Monkey: He is always beaten up by the Biker Mice ( and,on occasion, Charley ), insulted by his boss and Karbunkle and doesn't even get paid a dime for his efforts.
- Character Catchphrase: "Mister Limburger ain't gonna like this!"
- Cool Bike: Nooooope: he drives some kind of tricycle with an engine. Heck, he uses a lamp as headlight.
- Demoted to Extra: As with Limburger and Karbunkle, the Catatonians becoming the main villains of the 2006 series left him becoming a minor character who only appears a handful of times.
- Dragon Ascendant: He is not exactly Limburger second-in-command (Karbunkle probably fits the role a little better) but was promoted in charge of operations by Lord Camembert in "I, Greasepit" after Limburger's last failure.
- Dumb Muscle: Not very bright and serves as the brawn of Limburger's team.
- To give you and example, he admitted to have never finished third grade, as well as taking ten years to leave kindergarten.
- Even Bad Men Love Their Mamas: He occasionally speaks fondly of his mother. Funnily enough, the episode "Pitfall" implies she is a witch.
- Evil Virtues: He has many noble traits that lock him into the role of antagonist.
- Loyalty: No matter how badly Limburger treats him, he never betrays the latter.
- Diligence: He's a hard worker who values integrity.
- Temperance: He works for peanuts but is content because that's more than enough for meeting his needs of food, clothing, shelter, etc.
- Honesty: He can't tell a lie to his boss to save his life.
- Hidden Depths: He may be dumb as a stump, but he's a surprisingly good leader. "I, Greasepit" had him running Limburger's company, while Limburger himself was relegated to being his lackey. While he had no clue how to run a company, he was just smart enough to listen to those who do, and managed to impress the entire Plutarkian high-command, something Limburger himself can only dream of. It's only because Limburger sabotaged him by luring in the Biker Mice that the status quo was restored.
- Irony: He loves to do the traditional Plutarkian greeting, but is refused because he's not Plutarkian. Limburger hates the traditional Plutarkian greeting, but he's forced to do it because he is a Plutarkian.
- Mook Lieutenant: He's apparently the field commander of the biker gangs that Limburger often sends after the Biker Mice— but he really, really shouldn't be.
- Multiple Head Case: In "Too Many Limburgers Spoil the Cheese", his meddling with Karbunkle's duplicator ray causes him to gain a second head that he bickers with for the rest of the episode.
- Noodle Incident: Apparently, before becoming Limburger's lackey, he worked for Skeletor and The Smurfs.
- Number Two for Brains: He usually makes a mess out of his boss' carefully laid plans, yet he is still employed by him.
- Super-Strength: Dim-witted he may be, but he has shown to be able to bend and tear metal with his bare hands.
- The Pig-Pen: As noted, his body literally oozes oil.
- Throw the Dog a Bone: In the 2006 revival, he ends up somehow becoming the new CEO of Rump Industries. It's a far better ending then what happend to Limburger, Karbunkle and Brie.
- Villainous Crush: He falls for Charley when she sees her covered in oil in "Big Trouble". Fortunately for everybody, it was a one episode deal.
- What Kind of Lame Power Is Heart, Anyway?: Very rarely does his greasiness ever help him out.
- Walking Shirtless Scene: 99% of the time. The one time he does wear a shirt is in the episode "Hit the Road, Jack".
A hideous and masochistic being who serves as Karbunkle's guinea pig.
- Chuck Cunningham Syndrome: Save for a brief cameo as a hologram projection in "Once Upon a Time on Earth", he is nowhere to be seen in the 2006 revival.
- Extreme Omnivore: He once ate Karbunkle's counterfeit machine. He also devoured the blanket he was covered with in "Once Upon a Time on Mars".
- The Igor: He's a misshapen hunchback who helps out Dr. Karbunkle, mainly by volunteering to be used to test weapons and dangerous chemicals.
- Minion with an F in Evil: He cares more about getting himself hurt for pleasure than being of any use to Limburger and the other villains.
- Mixand Match Critter: Karbunkle sewed it together with parts from an octopus, a dog, a chicken andā¦ whatever the rest is supposed to be.
- Non-Indicative Name: He is not a mutant at all, but rather some kind of Frankestein's monster Karbunkle created to troll Limburger on Mars (before the scientist started working for him).
- Too Kinky to Torture: He thoroughly relishes every kind of pain that's inflicted on him.
- Villainy-Free Villain: He is on the side of the bad guys ā¦ on paper. In reality, he usually just cleans, drives and acts as a guinea pig for Karbunkle's inventions. It's pretty evident he is with Limburger just because it's the side where he is most likely to get hurt.
- Who Even Needs a Brain?: On occasion, his brain is removed or destroyed, and yet he's still able to function without a brain.
Another Plutarkian and a rival of Lawrence Limburger who is stationed in Detroit.
- Actor Allusion:
- When his regular mask was ruined in "Pwetty Wady" he tried others on: one looked like his voice actor, Luke Perry, while another looked like Ian Ziering, Vinnie's voice (ironic, seeing as Ziering would voice him in the 2006 sequel, making it an unplanned case of Foreshadowing).
- When Vinnie asked if he knew him from somewhere, he joked they might have attended the same high school. Luke Perry (Brie's voice actor) and Ian Ziering (who voices Vinnie) starred together in ''Beverly Hills, 90210".
- Ax-Crazy: He's completely crazy and recklessly violent to the extent that at one point, he even plays with a grenade after removing the pin.
- Batman Gambit:
- In his debut episode " The Motor City Maniac " he sent his goons to Chicago to attack Limburger's men to lure him in a fight to decide who keeps both Chicago and Detroit, knowing his army can easily beat Limburger's thanks to better weapons, training and commander.
- By "Back to Mars " he was well aware it was only a matter of time before the Biker Mice would nab Limburger and transport him to Mars for trial. Therefore. he beamed the Martian resistance manipulated videos showing the mice helping Limburger destroy Chicago to make them look like traitors. This way, both Limburger and the Biker Mice would have remained on Mars (or been executed), leaving him with no opposition to conquer Chicago.
- Again in "Hit the Road, Jack" when he pretends to demote Number One to make Limburger thinks his ex-dragon was ready to help him to get revenge on Brie, while they actually wanted to draw him to Detroit to be destroyed by Brie's new weapon. However he ended up with him getting Out-Gambitted.
- Big Bad Wannabe: Subverted in the 1993 series, where he's actually quite competent at his schemes and is arguably more of a threat then Limburger, with his plans usually only failing because of outside factors. It's played straight in the 2006 sequel series though: His one and only appearance in the series has him try to get Limburger arrested while Brie gets a reward from Lord Camembert in exchange for Limburger and the regenerator, except Camembert turns out to be a Catatonian Shapeshifter who ends up causing both Limburger and Brie to be captured and finally taken out for good. Even Limburger calls Brie out on his huge failure.
- Elmuh Fudd Syndwome: Aside from his short stature, another way he differs from most Plutarkians is that he always pronounces his R's and L's as W's.
- Evil Versus Evil: Despite them being both Plutarkians, Brie tries constantly to conquer Chicago from Limburger.
- Fish People: As with all Plutarkians, he is a fish alien.
- Forced Transformation: At the end of "Once Upon a Time on Earth", the regenerator is used to turn him into a non-anthropomorphic fish that can still talk.
- Genre Savvy: He knew Limburger would try to take him by surprise by attacking before the agreed time for the duel, so he was easily able to counter his " surprise attack " and win.
- Latex Perfection: Like Limburger and the other Plutarkians based on Earth, he wears a mask to appear human.
- Mad Eye: His right eye is yellow with a green swirl.
- The Napoleon: Appropriately enough, Napoleon Brie is short and also has big ambitions, considering he intends to take over Limburger's operations in Chicago.
- Narcissist: After taking over Limburger's tower in "Back to Mars" he immediately stuffed it full of statues and paintings depicting himself. He even put little masks of his face on the fishes in the tanks.
- Punny Name: His surname is Brie, a kind of cheese that is popular in France, while his first name refers to French emperor NapolƩon Bonaparte.
- Sanity Has Advantages: Apparently not as much as one would think: he is an absolute madman, but he is also on the good side of Always Someone Better to Limburger. "Diet of Worms" reveals he is one of the four best Plutarkian bosses on Earth (maybe even the best, since he was supposed to host their meeting).
Napeleon Brie's right-hand man.
- Badass Normal: He appears to be just a normal human unlike the majority of villains in the series, but he's still very capable of holding his own against the Biker Mice.
- Berserk Button:
- Losing his position as Brie'sā¦ well, number one. Limburger took advantage of this in "Back to Mars" by pretending to switch side and become Brie's Dragon, leading to Number One to shot his own boss.
- Again in "Hit the Road, Jack", when losing his position to Angela was enough to decide to help Limburger in overthrowing Brie. Actually a subversion, since it was part of Brie's Batman Gambit to lure Limburger and destroy him with his new weapon.
- Chuck Cunningham Syndrome: Despite his boss returning in the 2006 revival, Number One is completely absent in it.
- The Dragon: Serves as Napoleon Brie's right-hand man and is almost always at his side and leading Brie's goons whenever he shows up.
- Easily Forgiven: He shot his own boss during "Back To Mars" after being played by Limburger, yet in the following episodes Brie still has him on his payroll.
- Foil: Number One is pretty much everything that Greasepit isn't: Competent, smart, and treated with respect by his boss {And he's also actually paid for his work, unlike Greasepit}.
- Only Known by Their Nickname: He is only called by his title as Brie's right hand man.
- Psycho for Hire: Is under Brie's employment and has a bit too much fun doing his job, though he's more professional then most examples of this trope, and only turned against Brie when Limburger tricked him into thinking he was getting demoted.
A Plutarkian lawyer whose stench is so rotten that even other Plutarkians can't stand it.
- Alliterative Name: His given name and his surname both begin with the letter P.
- Amoral Attorney: He's a lawyer and every bit as dishonest and unscrupulous as your average Plutarkian.
- Evil Smells Bad: He's rather prideful of his stench, which grosses out even other smelly Plutarkians.
- Fish People: Like all Plutarkians, he's a fish alien.
- Latex Perfection: Much like Limburger and Brie, he wears a realistic mask to appear human.
- Lean and Mean: He's one of the few skinny Plutarkians in the show.
A Plutarkian stationed in Las Vegas.
- Cool Plane: He has a giant one (a close jet looked minuscule by comparision) which contains a casino.
- Eyepatch of Power: He wears an eyepatch, though the split second where his human mask comes off shows that both of his eyes are okay.
- Fish People: He's a Plutarkian, so of course he's a fish alien.
- Latex Perfection: Like all Plutarkians stationed on Earth, he wears a realistic mask to appear human. Unlike Gerald Gruyere and Gutama Gouda, we actually get to see him unmasked, albeit very briefly.
- Remember the New Guy?: The Biker Mice recognize him and Gerald Gruyere even though "Diet of Worms" is the only episode they make a full appearance in.
- Verbal Tic: Most of his lines contain allusion to gambling.
A Plutarkian stationed in New Orleans.
- Alliterative Name: Both his first name and his last name start with the letter G.
- Fat Bastard: Like most Plutarkians, he's quite chubby with an unpleasant attitude to match.
- Fish People: Being a Plutarkian, he's a fish alien.
- Ragin' Cajun: He's a villain with a Cajun accent.
- Latex Perfection: Like all Plutarkians stationed on Earth, he wears a realistic mask to appear human.
- Remember the New Guy?: The Biker Mice recognize him and One-Eyed Jack Monterey even though "Diet of Worms" is the only episode they make a full appearance in.
A Plutarkian stationed in Los Angeles.
- Alliterative Name: His given name and his surname both begin with the letter G.
- Deflector Shield: His crystal can do this, as well as shooting laser beams.
- Fish People: He's a Plutarkian, so it's a given that he's a fish alien.
- Hypocrite: Despite presenting himself as a peace loving hippie, he is usually well armed and he works for an evil galactic empire. It gets lampshaded when he gives Vinnie some plastic explosive and signs the note with a "Peace".
- Latex Perfection: As with all Plutarkians stationed on Earth, he wears a realistic mask to appear human.
- Lean and Mean: Another skinny Plutarkian.
- New-Age Retro Hippie: Has the dress and mannerisms of a stereotypical hippie, even mentioning such things as "karma" and "vibes".
- Pun: After losing the chance to become Earth's governor he complains he fells like " My Karma has run over my Dogma".
- Remember the New Guy?: Averted, which is notable because the other two new Plutarkian bosses in "Diet Of Worms" did get that treatment. The Biker Mice have no idea who he is when they see him arrive at Limburger's gathering.
A Plutarkian stationed in Cleveland who once used "Asphalt" Jack McCyber as a pawn in her ploy to usurp Limburger and Brie.
- Cast as a Mask: Her disguise as Angel Revson and her true self have separate voice actresses.
- Fat Bastard: She's quite plump in her real form.
- Fish People: She's a Plutarkian and therefore a fish alien.
- Holographic Disguise: Rather than wearing a realistic mask like the other Plutarkians stationed on Earth, Romana disguises herself with a hologram projection of a beautiful woman.
- Interspecies Romance: She faked being in love with Jack using her Holographic Disguise.
- Magnificent Bastard: The episode where she appeared was already a complex game played by several people... and she surpassed them all: she took advantage of Jack to obtain the super bike he was building, used Brie to have them build and then used Limburger to obtain them.
- Non-Mammal Mammaries: She's a fish alien with breasts.
- The Smurfette Principle: She's the only Plutarkian stationed on Earth who is female.
Lawrence Limburger's former boss and a Plutarkian stationed on Mars.
- Bad Boss: He clearly enjoyed making Limburger miserable. It's not without reasons, however: Limburger was constantly taking initiative behind his back, as well as scheming to take his place.
- Benevolent Boss: He was actually very nice to Karbunkle, always praising his work and never once did he insult or threaten him.
- The Cameo: In the 2006 revival, he makes a brief appearance in a flashback in the first part of the three-part episode "Once Upon a Time on Earth".
- Fish People: He's a Plutarkian and therefore a fish alien.
- Mysterious Middle Initial: It's not stated what the T stands for.
- Neat Freak: He carries around handkerchiefs to wipe messes all the time and at one point even chides Limburger for using the communications monitor without sterilizing the controls.
The leader of the Plutarkian race and the person that Limburger answers to.
- Bad Boss: He frequently yells at Limburger and forces him to do the traditional Plutarkian greeting even though Limburger doesn't like it.
- Demoted to Extra: While he wasn't exactly a main character in the 1993 series, he was a reoccurring minor antagonist who appeared in a decent amount of episodes and had plenty of screentime and interactions with Limburger. In the 2006 series he only shows up in 3 episodes, two of which are just voiceless cameos in flashbacks, and the one time he actually does get a speaking role, it turns out to not actually be him but instead a Catatonian Shapeshifter disguised as him.
- Fat Bastard: Like most Plutarkians, he is overweight and not a nice person at all.
- Fish People: Like all other Plutarkians, he is a fish alien.
- Greater-Scope Villain: Being the leader of the Plutarkian race and Lawrence Limburger's superior, he's technically the most important threat to the Biker Mice, but he only appears in a few episodes.
- Obnoxious In-Laws: He had a mother-in-law until she was indirectly killed by Limburger in "Stone Broke". Camembert rewarded Limburger by giving him his funding back.
- People's Republic of Tyranny: Technically, he is a public servant and he has to face elections every four year. In reality, he chooses his opponent himself (always some nobody who has no chance of winning) and after his victory he kills them. Everybody is on it, reallyā¦ he openly referred to the electors as "puppets" and got applauses, and a reporter covering the event called the election a "mockery of democracy".
- President Evil: He is in charge of a galactic empire whose only apparent economic strategy is "Let's invade other planets and steal their stuff", he is an ass to subordinates, kills every political adversary he defeats and keeps on stealing from Plutark's treasury.
- Swiss Bank Account: He deposits the money he regulary steals from the Plutarkian budget in a Swiss Cheese account, the Plutarkian equivalent of a secret hiding place for illicit funds.
A criminal who lives deep within the pits of Chi-town. He runs his own gang and kidnaps people to make his slaves.
- Arch-Enemy: Is this to Four-By, the man that started a rebellion in his domain. He also considers the Biker Mice to be this to himself, but they just see him as another villain to foil.
- Even Evil Has Loved Ones: His only good trait is that he sincerly cares about his daughter in the 2006 series, to the point that he's unwilling to kill the Biker Mice when she runs right in his crossfire to save Modo.
- Karma Houdini: His final appearance in the original series did have him and his men being captured and locked up by Four-By, but the sequel series revealed he eventually escaped. The 2006 series only gives him one appearance and he's never shown being captured or suffering any long term punishment.
- Large and in Charge: He's pretty tall when compared to his lackeys.
- Shock and Awe: His whip is electrified.
- Strong Family Resemblance: His daughter basically looks like a female version of him.
- Villain Team-Up: He strikes an alliance with Limburger in "Pitfall", helping him capture Provoloni in Exchange to help against Four-By's rebels. The sequel series also has him teaming up with Ronaldo Rump and using one of Rump's stores as a front for capturing and enslaving unsuspecting civilians, even having Catatonian robots under his command in addition to his human Mook's.
- "The Villain Sucks" Song: The album by Jeff Scott Soto has a song dedicated to condemning him and his actions called "Rockin' in the Pit".
- What Happened to the Mouse?: In the 2006 series he only appears in one episode and is never seen again after attacking Limburger and Karbunkle for their betrayal. What makes his disappearance even more confusing is that not only are his captured slaves shown free at the end of his only episode, but Limburger and Karbunkle appear again in the Grand Finale, yet the Pit Boss nowhere to be seen and isn't even mentioned.
A robotic thug who is also the first supervillain hired by Limburger to defeat the Biker Mice.
- The Ahnold: Appropriately enough, he's a caricature of Arnold Schwarzenegger, if only in hair color, build, and having an Austrian accent.
- Early-Installment Weirdness: His first appearance in the series' premiere episode "Rock and Ride" suggests he was built by Karbunkle. Later episodes made it clear that he is an independent mercenary.
- Starter Villain: He's the first supervillain hired by Limburger to fight the Biker Mice.
- Terminator Impersonator: He's a reference to the Terminator franchise due to being a bionic assassin. His last line in his debut is even "I'll be back".
A villain with magnetic powers.
- Amazing Technicolor Population: He appears to have purple skin except for his hands, which are green.
- Magnetism Manipulation: He is able to control anything made of metal.
- Weaksauce Weakness: Being near too much metal at once can cause the metal to stick to him and immobilize him.
A rat who likes to travel by digging tunnels.
- Berserk Button: Takes offense to being called a mouse.
- "The Villain Sucks" Song: The album by Jeff Scott Soto features a song about him entitled "Tunnel Rat".
- Verbal Tic: He pronunces "Right" as "Rat".
- You Dirty Rat!: He's a humanoid rat and a bad guy.
Individually known as Hacka and Honka, they are a pair of Plutarkian brothers who are smelly even by their species' standards.
- The Ahnold: They're both brawny and they both have Austrian accents.
- Evil Smells Bad: Even before Limburger schemed to clear out Chicago by making the brothers smell worse, their stench was considered too obnoxiously foul even to other Plutarkians.
- Extreme Omnivore: In the episode "Garbage Wars", they go from eating junk food to eating garbage.
- Fartillery: In their first appearance, Limburger attempts to use their gasshole traits to drive out the population of Chicago by sticking them in a truck so they could be transported around the city, spewing their belched stink everywhere and making the whole city smell so awful that no human could remain in it.
- Gasshole: Consuming junk food gives them upset stomachs, causing them to constantly emit loud, noxious-smelling belches. The more junk food they eat, the more they belch, and the nastier those belches get. At one point, the "second hand smoke" from a Loogie Brother belch forces the Biker Mice to don their space-faring helmet screens, and knocks Charlie unconscious.
- No Celebrities Were Harmed: They are based on Hans and Franz from Saturday Night Live, who themselves are parodies of Arnold Schwarzenegger.
- Odd Name Out: They appear to be the only Plutarkians who aren't named after a cheese.
- Theme Twin Naming: It isn't certain whether or not they're the same age, but their first names both begin with H and end with "ka".
- Trademark Favorite Food: Like the Biker Mice, they are very fond of hot dogs.
Evil Eye Weevil, a former stuntman who claims to be Elvis Presley's alien twin brother, is one of the recurring villains. He's very egocentric, snobbish and abusive. He possesses a special weapon called the hostility ray; when a person gets struck by its red beam, he or she becomes hostile toward other people — hence the name. When several people get hit by it, things can get quite nasty. Once, the hostility ray even made the Biker Mice, normally close friends, yell at each other and almost start a fist fight. Luckily, the ray's effects wear off with time.
Evil Eye has two minions who are known as the Pukes of Hazzard, a pair of purple skinned muscular dwarves. They admire their master and do nothing but bask in his magnificence despite they often get abused by him. They do any menial task Evil Eye sets before them just for the privilege, including preparing his stunt bike to fixing his limbs when he breaks them — which happens so often that it's become a Running Gag.
- Bad Boss: Treats the Pukes of Hazzard like garbage.
- Elvis Has Left the Planet: Played with. He claims to be Elvis Presley's alien brother.
- Eye Beams: The hostility ray is fired from his eye.
- Foil: Just like Vinnie he is egocentric, arrogant, obsessed with his look and the best biker of his group. Unlike Vinnie, he doesn't care for everyone else, consider his two companions minions, and regularly insults and abuse them.
- Hate Plague: His "Hostility Ray", which causes whoever gets hit to become angry and aggressive to whoever and whatever they have around. It's stated to last only a few minutes, but the exact time seems to depends by the character of the target: Modo was the first one to shrug it off, while Vinnie was still under its effect when he was captured.
- It's All About Me: He's even more egocentric than Vinnie.
- Jerkass: He's unpleasant and repugnant to the point that even Limburger dislikes him.
- Lean and Mean: He is very skinny and quite obnoxious.
- Meaningful Name: His name is "Evil Eye" and his eye fires a hostility ray that makes people hostile to one another.
- Names to Run Away from Really Fast: Evil Eye Weevil is a name that pretty much screams "not a pleasant person at all."
- No Celebrities Were Harmed: His goofy hairstyle and voice parody Elvis Presley, while his name and occupation as a stuntman are a reference to Evel Knievel.
- Skewed Priorities: Doesn't matter how often his bones are broken, his only concern is if his hair are alright.
- Terrible Trio: Forms this with his two minions.
A cowboy-type villain who can melt anything metal by touching it.
- Alliterative Name: Both parts of his name begin with the letter C.
- Cool Car: He has a stereotypical Texan car that shoots a corroding gas.
- Eyepatch of Power: He wears an eyepatch for some reason.
- Fangs Are Evil: His teeth are sharp.
- Logical Weakness: He can't control his ability to melt metal, so it is not a good idea to let him control a machine.
- Make Them Rot: His power is to make metal melt just by touching it.
The leader of a gang known as the Road Ravens, who were once hired by Limburger to steal gas from trucks.
- Fat Bastard: He's quite a hefty fellow, and nasty to boot.
- Large and in Charge: He's way larger than his minions.
- No-Sell: He doesn't even notice when Throttle sucker-punches him square in the jaw.
- Stout Strength: In spite of his obesity, he is quite strong, able to easily lift Throttle off the ground with one hand.
A pair of giant monsters who love causing destruction.
- Attack of the 50-Foot Whatever: They're usually quite large(bigger than Modo, ever). they can increase their size to the point to play with skyscrapers.
- Giant Equals Invincible: In their giant form, they No-Sell every attack.
- Pink Girl, Blue Boy: Kind of. Gorgonzola is aqua while Munsterella is magenta.
- Punny Name: Their names are a play on the cheeses Gorgonzola and Muenster.
- The Starscream: They were called by Limburger to destroy the Biker Mice. Once Limburger disappeared (due to a faulty transporter) they decided to conquer the planet themselves.
- Unholy Matrimony: It isn't exactly clear whether or not they're married, but they certainly act like a loving couple.
- Weaksauce Weakness: Making them laugh causes them to shrink.
A short woman Limburger once hired to keep it snowing on Christmas so he could continue harvesting ice for Plutark.
- Those Wacky Nazis: An affiliation with the Nazis is implied by her German accent and the fact that she's seen goose-stepping in a couple of scenes.
- Weather Manipulation: She can change the weather by slapping stickers of what she wants the weather to be onto a map.
The Pulverizer is an evil cyborg (or robot) with a militaristic theme both in his appearance and speech. He frequently uses military jargon in his speech. It is implied that he was once in the armed forces but became a criminal. When he was summoned, Karbunkle mentioned that the Pulverizer had just completed his six year stinct. The Pulverizer has an over sized right hand which is also his power fist. This very powerful weapon allows him to punch with outstanding power along with firing small bursts of energy.
The Pulverizer is a merciless, power-hungry and cruel bully and also a very competent leader. He's very cunning, not to mention fearless in front of his enemies. He is almost unstoppable when he has competent troops - this was seen in Cheese Cadets, when Limburger hired him Elite Mooks after his own goons went on strike.
- Ax-Crazy: He's aggressive, unstable, and not afraid to use physical violence.
- Blood Knight: In his own words:"This is war... I love it!"
- Drill Sergeant Nasty: He trains recruits harshly, and will not accept anything but the best.Limburger: They seem adequate.
Pulverizer:' I grind adequate soldiers under my boots! - Megaton Punch: He punched Greasepit with enough force to send him flying after he was summoned.
- Oh, Crap!: Gets this after he pushes Throttle's Berserk Button. Doesn't exactly say 'oh crap' but has a very terrified expression on his face.
- Power Fist: He has an oversized hand that gives him power.
- Red Eyes, Take Warning: His eyes are colored red.
- The Starscream: In his second appearence he uses Limburger's new Elite Mooks to take control of his empire and even get approval from Lord Camembert .
A villainess with a jackhammer as her weapon who was hired by Limburger during a Rushmore Refacement attempt.
- Berserk Button:
- She gets really mad when she isn't paid for her services.
- Also don't call her "babe": she is an artist.
- Multi-Armed and Dangerous: She's a villain with four arms and not one to be taken lightly.
- Multiboobage: She appears to have four breasts.
A robotic villain with fiery breath who can fly.
- Ace Pilot: Limburger actually calls him for his ability in piloting unusual crafts at first, before asking him to roast the Biker Mice.
- Breath Weapon: He breathes fire.
- Flight: He can fly.
- No-Sell: He is impervious to the Biker Mice's weapons, so they defeat him by baiting him into getting hit by a train.
- Sickly Green Glow: His eyes and mouth glow green.
A felinoid alien with the power to create mental projections.
- Aliens Steal Cable: Charley comes to the conclusion that he's familiar with the movies King Kong and The Alamo, and therefore able to create mental projections based on them, because he's seen the movies through cable.
- Amnesiacs are Innocent: After losing his memory, he immediatly stop attacking the mice. He only goes after Limburger and the others because Throttle convinces him Fred the mutant is his friend and they kidnapped him.
- Awesome Aussie: He speaks in an Australian accent.
- Easy Amnesia: After Vinnie docks him, he loses his memory ( but he still remember about his power, apparently ). The mice then convince him that Limburger and the others are his enemies.
- Cats Are Mean: He's an evil cat-man.
- My Brain Is Big: His brain is large and exposed, appropriately enough for a villain with mental powers.
- Reality Warper: He can make what he thinks about real (including changing his form).
- Punny Name: His name is a play on CAT Scan.
The heavyset leader of the Sand Raiders, a group of slave drivers on Mars.
- All There in the Script: He is only named in the end credits.
- Fat Bastard: Obese and an all around rotten guy.
- Shout-Out: His name is a play on Jabba the Hutt from Star Wars. Much like Jabba, he's also an obese alien and in "Back to Mars" he even attempted to enslave Charley and make her wear a revealing outfit as Jabba did with Princess Leia in Return of the Jedi.
A bird-woman bounty hunter who was once hired by Lectromag, Tunnel Rat, and others to do away with Limburger.
- Berserk Button: She never forgives people who make her lose her bounty.
- Bounty Hunter: Is her job. Specifically, she was hired to kill Limburger by the others supervillains he already hired, since he didn't pay them.
- Dark Action Girl: An amoral bounty hunter who is very good with weapons.
- Does This Remind You of Anything?: Her "passion" about cannons is...interesting, to say the least. Heck, she even licked her beak thinking about it, and specifically hoped they were "big cannons".
- Feathered Fiend: She's a villainous bird-woman.
- Non-Mammal Mammaries: She's an avian being with breasts.
- Screw the Money, I Have Rules!: She refused Limburger's bribe to let him live. She quickly changed her mind when the offer became "big cannons".
- Toothy Bird: She's a bird woman with teeth, which are mainly visible when she scowls.
- The Vamp: She uses her feminine wiles to manipulate Modo into helping her.
- Wounded Gazelle Gambit: She manipulates Modo into setting her free by pretending to be hurt by Throttle and Vinnie.
A group of alien hunters who try to hunt the Biker Mice at one point.
- Egomaniac Hunter: They hunt solely for the fun of it.
- Evil Sounds Deep: Their voices are very deep and sinister.
- Red Eyes, Take Warning: They're villains and most of them have red eyes.
A movie star who was once souped up by Dr. Karbunkle to destroy the Biker Mice when Limburger lured them into a trap with a job application while disguised as a movie director named Jack Romano.
- Ambiguously Evil: Was he truly trying to destroy the Biker Mice with the strength boost and weapons Karbunkle and Limburger gave him or did he think it was just all part of the movie?
- Bald of Evil: His hair is actually a wig covering his bald head.
- It's All About Me: Charley used to date him and regrets it because of how egotistical he was.
- No Celebrities Were Harmed: He is a parody of Jean-Claude Van Damme.
- Red Eyes, Take Warning: His eyes become red after what Karbunkle does to him.
- Small Name, Big Ego: He has a very inflated opinion on his lousy acting skills.
A villain driving a truck who was hired by Limburger to try and do away with the Biker Mice when he tried to drain Lake Michigan.
- Alliterative Name: Both parts of his name begin with the letter M.
- Ax-Crazy: He appears to enjoy using his truck to run people over a little too much.
- Fish People: He's a fish-like alien.
- Hoist by His Own Petard: The Biker Mice trick him into burying himself in mud using his own vehicle.
- Yellow Eyes of Sneakiness: He has yellow eyes.
A robotic double of Vinnie that Karbunkle built during Vinnie's attempt at tricking Karbunkle into constructing a replacement for Modo's robotic arm.
- Evil Knockoff: He's a robot who resembles Vinnie and is powered by his brainwaves.
A dragon from the in-universe comic strip Biker Knight, who was once brought to life using an invention of Karbunkle's to try and kill the Biker Mice.
- Our Dragons Are Different: A large green dragon who attacks by breathing fire from his nostrils.
- The Voiceless: He does not speak.
- Super-Toughness: According to Vinnie he is as tough as plutarkian steel, forcing the heroes to subdue him rather than beat him up.
- You Have Failed Me: Karbunkle erases his data from his machine after the Biker Mice defeat him.
Greasepit's favorite cartoon character. He was brought to life after Snortblast failed.
- Angrish: Like the character he is based on he occasionaly slips in this.
- The Bad Guy Wins: His own cartoon ends with him demolishing a city and celebrating it.
- Bottomless Magazines: As expected by a Yosemite Sam spoof, he never has to reload his gun.
- Captain Ersatz: He's a blatant parody of Yosemite Sam.
- Card-Carrying Villain: He straight up says he hates the good guys. In fact, it's the only reason he and Deathmaster fight the Biker Mice despite being angry with Limburger for calling them in the real world.
- Cartoon Bomb: He uses one.That can talk. And does its own countdown.
- Dual Wielding: He's armed with four guns, but only uses two of them at time.
- Family-Friendly Firearms: Averted:He's one of the few to not use a laser gun in the show.
- Genre Savvy: When he and Deathmaster trap the Biker Mice in a cage with a bomb and fly away, he actually considered if they should have stayed to be sure the mice get killed by that.
- Hoistby His Own Petard: The cage Deathmaster created falls on them, trapping them with Saddle Sore's dynamiteā¦ his lit dynamiteā¦
- More Dakka: His favorite method of attacking is by firing an assload of bullets.
- Straight Man and Wise Guy: The Wise Guy to Deathmaster's Straight Man
- Villainous Friendship: He tries to strike one with Deathmaster: he even defended him when the mice made fun of him, and later tried to make him relax with a joke. Unfortunatly the sorcerer didn't share the same feeling.
- You Have Failed Me: He and Deathmaster get their data erased after they are beaten by the Biker Mice.
Another cartoon villain brought to life to aid Saddle Sore.
- The Bad Guy Wins: Not against the Mice, but he apparently defeated the He-Man look-alike of his own cartoon, assuming that the attack was fatal and that the hero didn't recover from the attack off-screen.
- Captain Ersatz: He's most likely a representation of Skeletor from Masters of the Universe. He is even seen fighting a He-Man lookalike before Limburger and Karbunkle decide to make him real.
- Card-Carrying Villain: Just look at his name. Plus, he is furios with Limburger for bringing him away from his home, but he accepts to fight the mice just because they are the good guys.
- Evil Sorcerer: He can shoot lighting, fly, create something out of thin air and create barriers.
- Hoist by His Own Petard: The cage he created fall on him and Saddle Shore, just as Saddle was about to thrown his dynamite.
- Large Ham: He's pretty bombastic, especially when making death threats.
- Names to Run Away from Really Fast: Seriously, just read it.
- Never Say "Die": Averted: he can't apparently talk without mentioning Death every other sentence.
- Not Quite Flight: He can create a disk of energy and ride it in the air
- Oh, Crap!: "This speaks of bad things!", just as his cage fall on them.
- Obviously Evil: He has red eyes, he has a skull symbol on his chest, he wears a black cape that's red on the inside, and his face is a bit skeletal. You'd have to be a complete idiot to even think that he's a good guy.
- Red Eyes, Take Warning: His eyes are red.
- Straight Man and Wise Guy: The Straight Man to Saddle Shore's Wise Guy.
- Weaksauce Weakness: Without his sceptre he can't perform his spells.
- We Will Meet Again: He promised the Biker Mice they will see him again in their nightmares.
- Who Dares?: He combines it with an Implied Death Threat"What worm-ridden carcass would dare spirit me from my catacombs?"
- You Have Failed Me: He and Saddle Sore get their data erased by Karbunkle after the Biker Mice defeat them.
Copies of the Biker Mice created from drawings made by Greasepit after Snortblast, Saddle Sore, and Deathmaster prove ineffective in stopping them. For added nastiness, each was infused with the data of one of the previous three digitized creatures.
- Arm Cannon: Modo's duplicate robot arm doesn't contain oneā¦ it becomes one.
- Breath Weapon: The Duplicate Throttle, due to being fused with Snortblast the Dragon, is constantly exhaling gouts of flame.
- Evil Knockoff: They're essentially evil clones of the Biker Mice.
- Magic Knight: Modo's twin, since it has all of Modo's strength (if not more) plus Deathmaster's magic skills.
- Mix-and-Match Critters: Each Biker Mice Duplicate is a fusion of an evil clone of one Biker Mouse and one of the three cartoon villains from earlier in that episode. The Duplicate Throttle is fused with Snortblast, the Duplicate Modo with Deathmaster, and the Duplicate Vinnie with Saddle Sore. This affects their looks, although in a downplayed case of how this usually plays out, and speech patterns.
- No-Sell: They are completely immune to the Biker Mice's attacks. The heroes only won by using the duplicates' weapons against them.
- Not Quite Flight: Modo's twin can create the same disk as Deathmaster.
- Our Dragons Are Different: Due to how it was created, Throttle's duplicate is basically a mouse-shaped dragon.
- Shout-Out: While fighting Vinnie, his duplicate says:
- Terrible Trio: The Biker Mice are already a group of three, so of course evil copies of them would be a villainous trio.
- Voice of the Legion: The Modo and Vinnie duplicates are voiced by two voice actors speaking in unison; the original Biker Mice actor, and the actor for, respectively, Deathmaster and Saddle Sore.
A cyborg with power over machines.
- Bald of Evil: He hasn't a single hair on his head.
- Cyborg: He's a humanoid being with bionic parts.
- Technopath: He can manipulate anything mechanical.
- The Voiceless: He doesn't speak.
A trio of half-rat, half-bike creeps that Karbunkle created from the Biker Mice's DNA and scrapings from their bikes.
- Crippling Overspecialization: They are possibly better bikers than the Biker Mice; however, since they are fused with their bikes, they can't defend well when the mice and their bikes attack separately.
- Cyborg: They are technically cyborgs, considering that they are organic rodent-men from the waist up and bikes from the waist down.
- Evil Knockoff: Though they don't look anything like them, they are technically evil clones of the Biker Mice.
- Eye Beams: They can shoot lasers from their eyes.
- Red Eyes, Take Warning: All of them have red eyes.
- Screw This, I'm Outta Here: After being defeated, they flee from battle.
- Terrible Trio: Three villains cloned from the Biker Mice.
- The Voiceless: None of them talk, just howl.
- What Happened to the Mouse?: Or rat, whatever. One of them appears in "Diet Of Worms" working for Gouda, but the other two aren't seen or mentioned.
- You Dirty Rat!: In spite of being cloned from the Biker Mice, they look more like rats than mice.
An icy supervillain hired by Limburger while at the North Pole.
- An Ice Person: True to his name, he has power over ice, though he also can generate heat.
- And I Must Scream: Modo defeats him by deflecting his ice beam back at him, freezing him solid.
- Walking Shirtless Scene: He is completely bare-chested for the entire episode.
King Arthur's evil nephew, who aided Limburger in his scheme of stealing deposits of pure tin from Camelot via time travel.
- False Friend: Pretends to hate Limburger and he offers the mice his help, but he is actually in cahoots with the Plutarkian.
- Evil Nephew: He is the evil nephew of King Arthur.
- Taken for Granite: Morgan LeFay ends up turning him into stone by mistake.
- Unholy Matrimony: Implied with Morgan LeFay.
An evil sorceress accompanying Sir Mordred.
- One-Winged Angel: Turns herself into a large dragon during her final confrontation with the Biker Mice.
- Taken for Granite: After accidentally turning Sir Mordred into stone, Throttle uses a reflective shield to trick Morgan into turning herself into stone as well.
- Unholy Matrimony: Implied; Morgan seems to feel an attraction to Mordred, and is horrified when she accidentally turns him into stone.
- Voluntary Shapeshifting: She uses her magic to take on the forms of a crow and later a dragon.
A Nomad Rat who impersonated a Martian mouse so he could infiltrate the freedom fighters and warn the Plutarkian forces of when and where the freedom fighters would strike.
- Chuck Cunningham Syndrome: Even though Harley and the Nomad Rats return in the Grand Finale of the 2006 series, Mace is strangely completely absent and is never even mentioned.
- Cold Sniper: In at least one battle he shot against the other Freedom Fighters (Vinnie, to be precise) despite being still undercover.
- Combat Pragmatist: Since he is not a good fighter, he would happily use sneak attack, pick fight with someone physically weaker and shot from long distance at the first sign of the target.
- Greed: The only reason he agreed to help the Plutarkians in their war against the Martian mice: they pay well.
- Karma Houdini: He isn't seen getting any comeuppance for infiltrating the Martian mice on behalf of the Plutarkians or for kidnapping Harley.
- Kick the Dog: Considering he had a teleporter ready, he really didn't have a reason to kidnap Harley other than being a dick.
- Latex Perfection: He uses a convincing mask to disguise himself as a Martian mouse.
- The Mole: Pretended to be a Martian mouse to infiltrate the freedom fighters for Lawrence Limburger.
- Red Eyes, Take Warning: His real eyes are red.
- Smug Snake: When things started to go really well, his acting got less believable, making Throttle suspicious.
- You Dirty Rat!: He's a villainous rat.
Villains from the 2006 Series
Cataclysm is the absolute definition of Big Bad, both in role and size, although officially he is the second-in-command to Assistant Supreme Commander Hairball. He's larger than Modo, by several boot sizes, and his stooped-over stature and portruding fangs just add to the terrifying (but not truly ugly) exterior. Despite his alignment, Cataclysm isn't all bad - he still has a little inkling of love for his runt brother Hairball.
- Beard of Evil: He has a goatee.
- Big Bad Duumvirate: With Ronaldo Rump. While he is technically the second-in-command to Commander Hairball, he's far more competant and is a much bigger threat then his idiotic brother could ever hope to be.
- Cats Are Mean: He is a member of a race of cat-like aliens and is one of the major villains of the 2006 series.
- Dragon-in-Chief: He is technically Hannibal T. Hairball's second-in-command, but is obviously far more competent than him.
- Evil Redhead: He has red hair.
- Forced Transformation: In "Once Upon a Time on Earth", he ends up turned into a white non-anthropomorphic mouse.
- Genius Bruiser: He is very strong and is at least smarter than his brother Hairball.
- Large and in Charge: He towers over the Biker Mice and tends to call the shots.
- Walking Shirtless Scene: He is often bare-chested.
Cataclysm's runt brother, and emphasis on "runt". Hairball is Assistant Supreme Commander of Catatonia's invasion forces, and how he got there is a true mystery considering that he's missing a few brain cells.
- Aliens Of Russia: He speaks English with a Russian accent.
- Alliterative Name: His first and last names begin with H.
- Big Bad Wannabe: He is technically leader of the Catatonian invasion forces, but he's far too incompetent and dim-witted to get anything done without his brother's help.
- Butt-Monkey: Often gets humiliated and comically injured.
- Cats Are Mean: He is a member of a cat-like alien race and is one of the bad guys.
- Dumbass Has a Point: In "Driver's Ed", he reveals to know how the clutch works when the Biker Mice struggle to figure out how to drive a minivan. When asked how he knew, he simply states that he once oversaw the construction of a Catatonian vehicle that featured the use of a clutch.
- Even Bad Men Love Their Mamas: If the one time he threatens to tell his brother on her for destroying his parachute in "Vigilante Vengeance" is anything to go by.
- Fat Bastard: He's a bit chubby.
- Meaningful Name: Fittingly enough for his surname, Hannibal T. Hairball often coughs up hairballs.
- The Napoleon: He is very short, but has big ambitions.
- Villain Song: He performs a duet with Dr. Catorkian called "Dig Deep" in "Turf Wars".
A greedy billionaire who teams up with the Catatonians to fight the Biker Mice.
- Bald of Evil: He's a despicable man with a bald head concealed by a Dodgy Toupee.
- Big Bad Duumvirate: He and Cataclysm are both the main antagonists of the 2006 series.
- Corrupt Corporate Executive: He runs his own corporation and, among other dishonest doings, has sold swamp water as drinking water and attempted to destroy wildlife reserves so he can build an amusement park over the remaining land.
- Fat Bastard: There's a reason he's called Rump.
- Hartman Hips: Rare male example. His hips are considerably broader than his torso and rather femininely curved at that.
- No Celebrities Were Harmed: He's basically a Hispanic Donald Trump.
- Trumplica: If his name and appearance didn't give it away, then the above-quoted line probably did.
A Catatonian scientist who follows the orders of Ronaldo Rump and Cataclysm.
- Cats Are Mean: Like Cataclysm and Hairball, he is a member of a race of cat-like aliens and is a villain.
- Karloff Kopy: His voice, and to a lesser extent his face, is modeled after Boris Karloff.
- Large Ham: He tends to speak in a loud and melodramatic way, especially when he boasts about his inventions.
- Lean and Mean: He is extremely skinny and is one of the bad guys.
- Mad Scientist: Specializes in unorthodox experiments and creating weapons to fight the Biker Mice.
- Sudden Name Change: He gives his first name as "Cantankerous" in "Manchurian Charley", while "Bringing Up Vinnie" has him give his full name as "Phineas P. Catorkian".
- Suspiciously Similar Substitute: He's a Catatonian Dr. Karbunkle, right down to being taller and thinner than all of his superiors.
- Villain Song: Performs a duet with Hairball called "Dig Deep" in "Turf Wars".
Ronaldo Rump's British cousin who appears in two episodes.
- Beard of Evil: He has a beard.
- Corrupt Corporate Executive: Like his Hispanic cousin, Sir Richard Brand-Something runs a corporation and is very dishonest and corrupt.
- Evil Brit: Not surprising, considering who he's a parody of.
- Karma Houdini: He's one of the only two villains who doesn't get a permanent punishment for his crimes by the end of the series.
- No Celebrities Were Harmed: He is a parody of Sir Richard Branson.
Sir Richard Brand-Something's robot henchmen, who often assume the guise of human butlers.
- Battle Butler: Both of them are willing to fight the Biker Mice when Brand-Something gives the order.
- Evil Brit: Like their boss, they're bad guys who have British accents.
- Fat and Skinny: Bangers is gaunt while Mash is plump.
- Mecha-Mooks: They are robotic minions.
A pair of Catatonians who occasionally show up to aid Cataclysm, Hairball, and/or Rump.
- Cats Are Mean: They are alien cats and are underlings of the bad guys.
- The Ditz: They are both pretty dumb.
- Fat and Skinny: Puss is short and fat, while Boots is much taller and thinner.
- Punny Name: They're a play on the fairy tale Puss in Boots.
- Simpleton Voice: Boots talks with a goofy voice indicating his idiocy.
A Catatonian shape-shifter who once tried to deceive the Biker Mice by assuming the form of a Martian mouse and claiming to be Stoker's niece.
- Alliterative Name: Her first and last name both start with C.
- Cats Are Mean: She's a member of a race of cat-like aliens and is one of the bad guys.
- Head-Turning Beauty: Hairball and Cataclysm are shown to be attracted to her. The same goes for Throttle, Vinnie, and Modo in regards to her Martian mouse form.
- Southern Belle: Her Martian mouse form has her talk with a Southern accent.
- Villain: Exit, Stage Left: Her last appearance had her disguised as Lord Camembert to trick Napoleon Brie into helping them get the mice and the Regenerators. When the ruse was revealed, she simply walked off.
- Voluntary Shapeshifting: She can change herself to look like other people.
A lizard-man with flamethrowers built into his gloves who was once hired by Rump to snuff out Stoker.
- Fire-Breathing Weapon: He's armed with flamethrowers built into his gloves.
- Psycho for Hire: He's hired to do away with Stoker and he loves his job a little too much.
- Pyromaniac: He expresses a fixation on using his flamethrowers to burn Stoker and the Biker Mice to death.
- Reptiles Are Abhorrent: He's a villainous lizard.
A purple-skinned alien cyclops who can hypnotize whoever he makes eye contact with.
- Berserk Button: He really hates it when people mess up his tuxedo.
- Cyclops: He's a one-eyed alien.
- Hoist by His Own Petard: Charley ends up using a hand mirror to trick Eyemore into hypnotizing himself.
- Hypno Fool: He uses his hypnotic gaze to make Charley sabotage the Biker Mice's bikes and to make Vinnie act like a chicken and pay him the money needed for a new tuxedo.
Hairball's half-brother.
- Cats Are Mean: He's a villainous cat alien.
- Fat Bastard: He's just as pudgy as Hairball.
- French Jerk: He speaks in a French accent and is quite rude.
The leader of a gang of punks called the Nitros, who harassed a woman named Tanisha before Modo intimidated him into leaving her alone.
- Bald of Evil: He's a bald and aggressive thug.
- Paper Tiger: He acts like a dangerous criminal, but becomes frightened out of his wits once Modo makes it clear he won't be having any of his threats.
- Scary Black Man: He's a black criminal who at least has the facade of being fierce.
- Sunglasses at Night: Constantly wears sunglasses for no apparent reason.
A Catatonian surfer on Saturn's moon Titan who was secretly still loyal to the other more malicious Catatonians.
- Cats Are Mean: He's a cat alien who isn't loyal to the other peaceful Catatonian surfers and is actually subordinate to the more malicious Catatonians.
- Lean and Mean: He's stringy and completely treacherous to the other Catatonian surfers.
- The Mole: He lived with the Catatonian surfers while secretly planning against them to aid the more malevolent Catatonians.
- Rewarded as a Traitor Deserves: When he bows to Cataclysm after having delivered his fellow surfers, Cataclysm mocks him and kicks him back over with the others, saying he doesn't like traitors.
- Surfer Dude: Like all Catatonian surfers, he talks like a stereotypical Californian surfer.
- What Happened to the Mouse?: Is never seen after getting knocked out by the surfer kid. We don't know what if any punishment he received.
A bounty hunter who once tried to capture the Biker Mice for a $1 million cash reward on Ronaldo Rump's reality show Vigilante Vengeance.
- Animal Motifs: In addition to actually owning a pet dog named Spike, he also has dog-like facial features and howls a lot.
- No Celebrities Were Harmed: He is a parody of Duane "Dog" Chapman.
A beefy guy at a junkyard with multiple faces who tried to help Ronald Rump crush the Biker Mice after getting their motorcycle licenses revoked.
- Ambiguously Human: Looks human enough but has a revolving head with five faces resembling four presidents and one vice president.
- No Celebrities Were Harmed: His faces and the voices associated with them are clearly based on former Vice President Dick Cheney and former Presidents George H. W. Bush, George W. Bush, Richard Nixon, and Bill Clinton.
A Martian mouse who became a space pirate and holds a grudge against Stoker.
- Alliterative Name: Both her given name and her surname start with M.
- Evil Aunt: She turns out to be Vinnie's maternal aunt.
- FaceāHeel Turn: She used to be among the Martian freedom fighters, but turned rotten out of jealousy over Stoker being chosen to be the freedom fighters' leader.
- Fat Bastard: She's quite rotund.
- Space Pirates: She leads a crew of Martian mouse space pirates.