Every sixty years, many tribes gather together to compete in a competition known as 'the Great Juju Challenge', where the winning tribe would gain the favor of the Moon Juju (Tina Illman) until the next competition. In the current time, Tak (Jason Marsden) and Lok (Patrick Warburton) represent the Pupanunu tribe as they compete against three other teams; Jib and Jab (JAQ) of the Jibbajabba Tribe, Stone Crusher (Edie McClurg) and Thunder Fist (Mitzi McCall) of the Grammazons, and Bartog (Andre Sogliuzzo) and Crug (Dee Bradley Baker) of the dreaded Black Mist tribe. As Tak and Lok progress through the challenge, however, a sinister conspiracy that concerns the Moon Juju’s past and the ultimate fate of the Pupanunu people seems to be afoot...
Tropes include:
- Actor Allusion: Tak says "I'll knock you into next week" while driving. Jason Marsden did voice Max from the movie, not Pete.
- Announcer Chatter: The Announcer Juju does this during levels, especially when he talks about upgrades.
- Ascended Extra: The Moon Juju is a central role in the game rather than a plot device or cameo.
- A Winner Is You: Actually, you don't even get that much. After winning the Challenge, Tak and Lok return to the Cantina to learn that everyone went home early "To beat the traffic" and there's not going to be a victory party because the Pupununu Tribe went over their budget. Jibolba blows a noise maker (once), Tak and Lok do a high-five, and the credits roll.
- Big Bad: Travis the Dark Juju.
- Breaking the Fourth Wall: As in the first game, Jibolba talks to the player.
- The Bus Came Back: The Two-Headed Juju and the Mummy King from the first game reappear after two years.
- Car Fu: The boss fights are demolition derbies between the teams.
- Contrasting Sequel Antagonist: While Travis lusts for the Moon Juju like Tlaloc, he is a corrupt juju who has been placed under house arrest and is known for cheating.
- Co-Op Multiplayer: Player 1 plays as Tak and Player 2 plays as Lok.
- *Cough* Snark *Cough*: *cough*Loser!*cough
- Covered in Gunge: A bit of Toilet Humor at the beginning of the game. As usual, Jibolba summons the player to his world. Unlike the last two games, they are not in his hut this time;Jibolba: You're in the Pupununu People's pig pen, and you're covered in Pupununu People's pig poo-poo!
- Demoted to Extra: The Woodies from the second game only make an appearance at the very first level.
- Elite Mook: Tak and Lok encounter giant Rockers starting with Temple of Light.
- False Teeth Tomfoolery: The Grammazons' true ages are made apparent when Lok tries to flirt with them and learns that they wear false teeth. The Dark Juju Travis later takes Thunder Fist's dentures and plants them to frame the Grammazons for cheating.
- Forced Tutorial: The tutorial level takes place at Feathercrag, where Tak and Lok test their abilities on Woodies.
- Foreshadowing: Crug refers to the Moon Juju as "mon cherie" in a French accent.
- Heel–Face Turn: Tlaloc has become this sometime in-between 2 and The Great Juju Challenge.
- Hollywood Drowning: When falling into water, Lok flails his arms to swim, but it is not enough, causing him to die.
- Hub World: The Juju realm serves as the location to go to other levels.
- Kill It with Water: Lok loses a life if he falls into the water.
- Lighter and Softer: Notably more so than the first two games thanks to Lok sharing the spotlight and the stakes being reduced.
- Love at First Sight: Lok is attracted to the Grammazons when he first saw them. (Though Tak believes that Lok was attracted to the Black Mist folks.)Lok: Tak, I have been smitten by love.
Tak: With the Black Mist?
Lok: No! - Older Than They Look: The Grammazons are senile and talk like elderly women, despite their youthful beauty.
- Old Save Bonus: Players get bonuses when they import a save file from Nicktoons Unite!, Barnyard and SpongeBob SquarePants: Lights, Camera, Pants!.
- The Phoenix: Tak and Lok have to follow a phoenix in the tutorial. It turns out they need a feather to join the Juju Challenge.
- Rock Monster: The Mooks encountered in the game except the tutorial are Rokkers.
- Score Multiplier: The rest of the time not used in levels multiply the final score of each level.
- Shout-Out:
- In a dark cave, Lok says "I've got a bad feeling about this."
- One of the levels is named Kiro Biro.
- Shown Their Work: The mammoths in the game are shown living in warmer climates like some species of mammoths did in real life, rather than the snowy environments mammoths are usually associated with in media. As a result, they are hairless as modern elephants rather than woolly.
- Sidequest: There is an optional quest to go back to the Gates of Nocturne and go to Ambush Grove, where Crug reveals to Tak and Lok the location of the Ancient Hammer of Handy Juju.
- Starfish Language: Team Jibba Jabba speaks by beatboxing.
- Super Drowning Skills: Played With. Lok is unable to enter water or he will die, not so much because he can't swim, but because fish despise him and will drag him under to drown him if he gets in.
- Walking Spoiler: Travis the Dark Juju.
- Weaksauce Weakness: Water to Lok.
- What Happened to the Mouse?: The story ends surprisingly abruptly once the Dark Juju is beaten, just cutting to Tak and Lok returning from the games triumphantly. We don't know what happens to Travis or the rest of the Black Mist.
- Wham Line: When Bartok gloats that the Black Mist plan on using Moon Juju's blessing to destroy the Pupununu People's village, Tak is horrified when Moon Juju can't bring herself to disagree. Then Jibolba explains..."She has no choice. The Moon Juju must serve and protect whoever wins The Great Juju Challenge. If they are evil, she must do evil."
- You Have Failed Me: Travis and Bartog have disallowed Crug for being the weak link of the Black Mist.
