Since Vine Realms is largely based on streams of video games by a group renowned for being a Fountain of Memes, there is a tremendous number of loving references to the games and other media that make the group popular. As such, nearly all of them technically double as Mythology Gags as well.
- The game's visuals are heavily inspired by the Paper Mario series.
- Characters speak by grunting in various pitches, which seems to be based on Banjo-Kazooie. Said game is an almost universal favorite among the Vinesauce community.
- The starting Avatar, and the only one unique to the player, is based on Toad but with a Vineshroom head.
- In the 🆑🆎 Club, the walls will sometimes display a scrolling banner of Sidesteppers.
- The Happy Emote Salesshroom is based on the Happy Mask Salesman.
- There is a bus, in the desert, that the driver says takes 8 hours to drive without taking your hand off the wheel.
- Vapor Zone has a restaurant called Papa Dook's Nuclear Pizza with a menu item called Pizza Cake.
- Other menu items include the Hot-Soon-Eh, Porkchop Sandwiches, and Steamed Yamsnote .
- The arcade upstairs in the pizza parlor has fictional game company logos based on Nintendo, Konami (which doubles as a shout-out to Hideo Kojima), Midway Games, and Capcom.
- There's also a ballpit in the arcade which V-Dub claims he poured "Mountain Doot" into, referencing "Vomit in the Ball Pit" by Red Vox, which Vinny is a member of.
- There's a purple ogre in the Cave of the Cursed called Mr. Shrink.
- There's a muscular man lifting weights on the O'Sea beach with a blond mullet and red trunks, named Bulk Bogan.
- There's a small green man in the Forgotten Forest named Yoba who speaks with a weird inflection.
- Yet another character named Toad, except based on a swordsman.
- Right next to "Toad" is his travel partner Krowno, who also has super spiky hair and a monkey tail.
- The Psychedelic Swamp has a resort called Spa Diva, as well as a purple frog named Deevo. There are also two ducks in the Vinetoad Kingdom fountain, a Shockingly Realistic Duck with a sweaternote and an Alarmingly Realistic Duck with a helmet and Blush Stickersnote .
- There's a large, colorful mascot rabbit in the Cave of the Cursed that has a giant centipede crawling into the zipper on its back, referencing jokes about Zipper T. Bunny hiding an Eldritch Abomination within his suit.
- There's also a familiar looking fringe-necked frog named Kramlet, as well as another named Kermeat.
- Fast Hedgehog runs around the O'Sea beach talking about chili dogs. There's also a checkerboard patterned section of Vapor Zone that has a flat-topped loop with gold rings in it, which a blue ball will roll around. Fast Hedgehog's design is also loosely based on the Sanic meme.
- A lighthouse sits at one edge of the beach, with its keeper demanding people admit their fondness for the lobster he sells.
- Sands, who can be found both wandering the beach and appearing behind the bar in 🆑🆎 Club, is a crab-themed parody of Sans from Undertale, right down to using the same voice grunts (themselves sampled from the SpongeBob SquarePants episode "Something Smells").
- Inside the Cave of the Cursed is a coffin adorned with the letter T, a handful of roses, and a football.
- The characters from Space Kids are locked up in the jail in the cave, and the boy can be unlocked as the only 3D player avatar.
- Several characters from The Gigglebone Gang are referenced, primarily the obnoxious frog Bunji.
- Save Pig appears near the desert bus as one of very few 3D characters, and one of very few completely overt inclusion of a game character. There is also the NPC Dingle, who hangs from a balloon in Vinetoad Kingdom and talks about fairies and making maps.
- There are three giant "Meat Worms" looming over the mountain in the distance, much like the one that appears at the beginning of Mario's Mystery Meat.
- "Bepis" has a mascot that looks like a metallic man drinking soda, who talks about having a game where he runs and collects cans.
- The Philosopher Shroom above Bepisman talks like the characters in Hylics, using the same kind of Purple Prose. Some lines are barely-altered paraphrases from the two games.
- The Aesthetic Shroom paraphrases the infamous Twitter post lambasting people who played Sekiro: Shadows Die Twice using mods and cheats to make the game more forgiving, which was a controversy at the time due to Sekiro's greater emphasis on story compared to other games by FromSoftware making some feel they were being "excluded" for not having the time or ability to develop their skill at the game.
- Two crabs in 🆑🆎 Club are named and modeled after Sephiroth and Cloud from Final Fantasy VII, with their dialogue referencing the remake. "Cloub" also talks about the flower girl Toad in Vinetoad Kingdom, who is named Claireth.
- One of the NPCs is called TamagoPet, with its story being that its friend disappeared one day and it appeared in the Vapor Zone.
- Crusty Buddy, a floating slice of pizza with googly eyes, is a combination shout-out to Clippy the Microsoft Office helper and BonziBuddy, the desktop assistant/malware program that got renewed interest due to his frequent inclusion in Joel's Windows Destruction streams.
- In the Vinetoad Kingdom tavern, there's a man with a sideways, realistic-looking fish head named Sushi. He appears to be a reference to the Realistic Fish Newscaster from SpongeBob SquarePants: Battle for Bikini Bottom.
- A ripped poster in the desert town references Raid: Shadow Legends, with part of its logo visible but damaged, claiming that the town is sponsored by them (given their tendency to sponsor anyone with a pulse). It even says "Get started for free today!" at the bottom.
- The "Starter-Pack" offered by one vendor has the same contents as in Dungeons & Dragons and even states that the pack is version "5e".
- One guard will sometimes sarcastically ask if someone stole your sweetroll.
- A slime just outside their village in the Psychedelic Swamp will tell you that "YOU CAN'T HANDLE THE JUICE!"
- The giant sword laying in the desert sands behind Pagona's Temple appears to be one for the one in Gerudo Desert.
- The floating islands that make up the Crossroads, as well as the Crossroads Toad's explanation for how they ended up that way, are based on Bastion.
- The champion of the slimes is named Pog and is making a particular self-satisfied expression, based on the original design of Twitch's "PogChamp" emote.
- "Krowno" mentions an "ocarina that allows time travel," which he finds ridiculous.
- Kramlet will sing the first few bars from The Rainbow Connection before saying, "Anyway, here's 'Wonderwall'." "Niam" will also describe the wall around the town in Lupin Desert as "Wonderful".
- The flavors of Bepis available from the machine:
- Garlic, with a purple W for its logo.
- Pigeon, with a red-and-white umbrella.
- Slime, with a block with eyes on its label.
- A clear bluish colored one with a pink core floating in it, labeled as Chaos.
- Among the references on the Town Crier's stand:
- A copy of Vinetendo Power magazine.
- A book titled Beat the Meatles, which both plays off The Beatles' Meet the Beatles!
and references an infamous New York Post headline about a lewd band story Paul McCartney recounted. - A tabloid with an article titled "Head like an orange" and a newspaper with another titled "Lil' monkey fella", both referencing Karl Pilkington and his appearances on The Ricky Gervais Show.
- DesertP's in-game character is teaching Naru-Toad the Kamehameha.
