- Experimental Film: Lois compares to the empty streets at night to London in 28 Days Later.
- The music video for LMFAO's "Party Rock Anthem", after a "28 Days Later" time card, has the duo waking up from a coma in an empty hospital just like Jim.
Video Games
- Left 4 Dead made extensive references to the film. To the point it was a borderline Homage, particularly in the No Mercy campaign.
- My Little Pony: Friendship Is Magic: The episode "28 Pranks Later", a spoof of zombie media in general, is named for this movie.
- The Simpsons: In "Treehouse of Horror XX", the segment Don't Have a Cow, Mankind is a parody of this film.