Literature
- The Laundry Files: Main protagonist Bob Howard's best friends, and roommates at the start of the series, are a gay couple nicknamed Pinky (Camp Gay) and the Brain (Straight Gay).
Video Games
- In Escape from Monkey Island, examining a dead rat leads Guybrush to say "What are we going to do tonight? Try to take over the Caribbean!
- In Infinite Craft, "Pinky and the Brain" is a possible crafting recipe.
Web Animation
- Camp Camp had in one episode a mouse with a pinky finger for a head and another with an enlarged brain labeled "taking cartoons literally".
- Idol Corp: Roca Rourin's backstory, as revealed on her debut stream, consists of being summoned by a pair of Pinky and Brain expies from a rock pet game.
Web Videos
- Bum Reviews: In the review of Kung Fu Panda, Chester A. Bum describes the character of Master Shifu as "A mousie who looks like Pinky and the Brain's lovechild."
- The Nostalgia Critic:
- When reviewing The Purge, the Critic had to deal with a rumor that Pinky and the Brain were splitting. It even led to an animated segment where the mice had an argument, featuring the voices of Rob Paulsen and Maurice La Marche themselves!
- The Critic has reviewed the Pinky, Elmyra & the Brain spin-off.
- Post Modern Jukebox covered the show's theme
... while featuring Rob Paulsen and Maurice La Marche as the mice in "human outfits", working as bartenders.
Western Animation
- A late Season 2 episode of Grossology is titled "Pinkeye and the Brain".
- MAD: One skit featuring Mickey Mouse as a Pest Control member has Pinky and the Brain among the mice and rats that Mickey catches.
- My Little Pony: Friendship Is Magic: In "The Ending of the End - Part 2" after Twilight's return to form, Pinkie asks, "What are we gonna do, Twilight?" Twilight replies, "The same thing we do every time, Pinkie: Try to save the world!"
- Robot Chicken:
- A sketch from "Kramer Vs. Showgirls" has Michael Moore interview Pinky, who reveals that the Brain's big noggin was the result of Paget's Disease. Pinky also reveals that he has a giant human ear on his back, a reference to the "Vacanti mouse"note .
- A sketch from "Eviscerated Post-Coital by a Six-Foot Mantis" features Pinky and The Brain going on a psychedelic trip. Maurice LaMarche reprised his role as The Brain for this sketch.
