Azumanga Daioh's Improbably Female Cast would inevitably have at least some Les Yay.
- Sakaki is beloved to the point of Even the Girls Want Her, in one instance having an enormous mixed-gender crowd following her trying to get her to join their clubs, and she's frustrated by the groups of female classmates continually squeeing over how cool she is (not because they're girls, but because she wants to be cute instead of cool).
- Minamo and Yukari are Heterosexual Life-Partners at the very least, one rarely seen without the other unless they're at work, closely involved in each others' personal and professional lives with a relationship that stretches back over a decade. Despite Minamo's constant frustration with Yukari's antics and the trouble she causes, she's always willing to help Yukari and shows no sign of ending the relationship, and for all Yukari's bluster it's clear she cares for Minamo in turn.
- Yomi and Tomo are almost a younger version of Minamo and Yukari, complete with similar personalities and the same Odd Couple dynamics. When told that the older pair have been together since high school, Tomo proudly boasts that she and Yomi have been together since grade school.
- In general, Tomo seems obsessed with boob sizes to the point where it shows less about her jealousy and more about just how long she spends staring at the other girls' breasts.
- Osaka develops a weird obsession with Chiyo, the latter repeatedly showing up in her dreams and having the most Imagine Spots about her, while Chiyo does her best to include Osaka and tries to understand her better. She also develops the same overprotective instincts towards Chiyo that Sakaki does, which results in a funny scene where Osaka rushes to save Chiyo from danger, complete with dramatically shouting her name and leaping in slow motion, only for Sakaki to casually help her while Osaka struggles to get past Yomi.
- Osaka's breast fixation sometimes surpasses even Tomo's. At one point in the third Beach Episode she just stares at Sakaki's breasts for a good ten seconds before declaring her jealousy, and a joke set up at the beginning of the episode ends with Osaka gazing down Kagura's shirt and proclaiming "Beans with rice... super-sized".
- There's a scene where Chiyo playfully places a sleeping Necoconeco doll on a napping Osaka's head. Sakaki then does everything within her power to keep the image, preventing the doll from falling and Osaka from waking up. Sakaki must find Osaka pretty dang cute to be that determined.
