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  • Practically every scene involving Teddy Brewster:
    Teddy: CHAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARGE! CHARGE THE BLOCKHOUSE!!!!
  • Cary Grant gives us a beauty of a Double Take when he first discovers the body in the window seat.
    Mortimer: When the curtain goes up the first thing you see is a dead body. (opens window seat, looks inside, then closes it.) The next thing...(swiftly opens it again) Hey, Mister?...
  • Teddy's encounter with Jonathan:
    Jonathan: "Go to bed, Teddy."
    Teddy: "Who are you?"
    Jonathan: "I'm Woodrow Wilson. Go to bed."
    • Teddy says Jonathan isn't Wilson, but he might be someone from his Africa trip.
    Teddy: You look like someone I might meet in the jungle.
  • This exchange:
    Mortimer: Aunt Abby, how can I believe you. There are twelve men buried in the cellar and you admit that you poisoned them.
  • Aunt Abby leads Aunt Martha out to see the "total stranger" that is in the window seat, prompting both Mortimer and Jonathan to rush to the window seat to prevent her from seeing the body of Mr. Spinalzo. The comical look of dawning comprehension on Mortimer's face as he realizes just who is responsible for the latest corpse must be seen to be believed.
    Aunt Martha: Spinalzo?
    Aunt Abby: I knew he was a foreigner.
  • Jonathan slowly coming to the realization that he and his kindly old aunts have murdered the same number of people.
    • Especially the ensuing "score tallying" scene:
    Dr. Einstein: Johnny, don't brag! You got twelve, they got twelve; the old ladies is just as good as you!
    • Einstein insists that Jonathan can't count the one in South Bend, since he died of pneumonia. "He wouldn't have died of pneumonia if I hadn't shot him!"
    • Meanwhile the aunts are looking quite flattered.
    • And of course, Jonathan’s frustration that while he's been hunted like a dog for years due to his murders, his aunts have never been caught and are still living their happy lives.
  • The aunts' latest "charity case" is handed a glass of the elderberry wine while Mortimer tries to reach the sanitarium on the phone. After hanging up, he distractedly grabs a glass of his own. The aunts warn him off, he realizes what he's doing and puts the glass down...and seeing the visitor about to drink, lets out a piercing shriek of horror.
  • "Insanity runs in my family. (Beat) It practically gallops!"
  • Mortimer's Sarcastic Confession. Cary Grant has never been Hammier.
    Mortimer: (singing) "There is a Happy Dale far, far away!"
    • Dr. Witherspoon's reaction to it is just as funny:
    Lt. Rooney: Look here, doc, couldn't you also make room for the ladies (at Happy Dale with Teddy)?
    Dr. Witherspoon: Just the ladies?
    • At one point during Mortimer's rant at the top of the stairs, Teddy comes out of his room, watches him for a while, and then goes back in making a "he's screwy" gesture.
  • Mortimer's increasingly funny Sanity Slippage. He goes from suave, assured on-top-of-the-world Only Sane Man, then discovers he's the Only Sane Man (because for that to happen, there's the rather unpleasant discovery that everyone else is insane, including all of his family, and it may be hereditary), and the rest of the movie is basically him not dealing well with that information while managing about three or four plotlines at once. By the end, he's been morphed into a high-strung, Seen It All man who's over the moon to find out he's not in hereditary danger of a mental disease, but may have been driven to one anyway.
  • Teddy's initial reaction to meeting Dr. Witherspoon:
    Teddy: Is he trying to move into the White House before I've moved out?
    Mortimer: Who, Mr. President?
    Teddy: (points accusingly at Witherspoon) Taft!
    Witherspoon: (indignantly sucks in his gut)
  • Mortimer's epic Lampshade Hanging, in which he talks about how characters in plays always act like idiots - and accidentally gives himself as an example.
    • He even gives Jonathan some ideas
  • Dr. Einstein's exit, where he quietly and nervously makes his escape while everyone else seems completely oblivious about what he's doing.
    Aunt Martha: Dr. Einstein! Are you leaving us?
    Dr. Einstein: Yes, please.
    • The lieutenant even reads of a description of him from his wanted poster while he's standing right there without realizing it.
    • Culminating in his nervous little laugh as he departs. It really must be seen to be appreciated.
  • The whole fight between Jonathan and the cops, which Mortimer watches disinterestedly from the safety of the stairs.
    • Mortimer makes a phone call in the middle of the fight. Jonathan picks up the phone to use as a weapon, then obligingly holds it higher when Mortimer asks him to.
  • Just after Mortimer has successfully committed Teddy and his aunts to the asylum without revealing they have murdered a dozen people, Elaine discovers the bodies and runs up screaming, nearly blowing the whole thing. Mortimer shuts her up kissing her repeatedly and carrying her out of the house.
    Lt. Rooney: "What's wrong with her?"
    Mortimer: (still muffling her screams with his lips) "It's way past her bedtime!"
  • The end line, as Mortimer is making out with Elaine:
    Cab Driver: But Mr. Brewster...!
    Mortimer: Oh, no! I'm not a Brewster! I'm the son of a sea cook! (throws Elaine over his shoulder and runs down the street) CHAAAAAAAAAAAAAAAAAAAARRRGE!
    Cab Diver: (calling after him while holding his arms in the 'tea pot' pose) I'M NOT A CAB DRIVER I'M A COFFEE POT!
  • Any time Cary Grant does a Double Take.
  • Jonathan has Mortimer tied up and gagged and is about to torture him to death when Dr. Einstein finds the poisoned wine and suggests a toast. Just as he and Jonathan are about to drink, Teddy blows a loud bugle signal outside, making them both jump and drop their glasses... and Mortimer goes right back to looking terrified.
  • The cab driver who apparently spends the whole movie waiting outside (with the meter running) for Mortimer and Elaine so they can go on their honeymoon. By the end, they’ve apparently racked up enough to buy the cab.
  • A policeman, about Teddy: "He thinks he's Teddy Roosevelt. So what? There's a lot of worse guys he could think he was."
  • Mortimer's reaction when he first sees Jonathan: "What's that?" Followed later by "It talks!"
  • When Mortimer calls Witherspoon to get Teddy committed, Witherspoon delicately notes that they already have several patients who think they're Teddy Roosevelt, and suggests that Mortimer try to convince him that he's Napoleon instead so as not to cause conflict.
    Witherspoon: Oh, dear. Another Roosevelt...
    • When Witherspoon arrives to pick Teddy up, he gets a shock when Teddy does his typical 'San Juan Hill charge' up the stairs. "Oh dear. And Happy Dale is full of staircases!"

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