Big-Lipped Alligator Moment: Done intentionally in "Pelvic". Xigbones and a band of skeletons abruptly break out into a funky disco tune for a single song and go back to normal the instant it's over.
"Exclusion Zone", the last song of Week 4. After the conspiracy theorist sips his coffee, the song speeds up significantly and the arrow density also increases a lot. Given most of the other songs in the mod are slower and have few if any note dense sections, it's a very heavily whiplash.
The 3.0 update revamped Week 2 and introduces "Crack", the third song of the week. "Confidential" and "Aegis" were slow and had fewer notes to offset the agents' gimmicks, but "Crack" is faster-paced and has more note dense sections. Compared to the other agents' gimmicks, which weren't too different from the normal gameplay, Agent Cuddles messes with your interface, whether it be covering your screen with static to obscure the arrows, moving the entire HUD to a different point on the screen, arrows and all, or lowering your volume so you can't hear the song at all. When he decides to mess with the interface is also completely random. The song after, "Enforcement", is a cakewalk in comparison.