After seeing 2048: Nowhere to Run in which he was shown to be a Gentle Giant, Replicant Sapper Morton's death at the hands of K comes off at this.
The skeleton K found buried under a tree at Sapper Morton's farm is revealed to be Rachael, who died giving birth.
K's breakdown when he discovers that his childhood memories are actually real (or so he thinks), which means he was born, not created, and therefore he's going to be hunted down if the truth comes out.
His reaction again, when he is told that his real memories never belonged to him and that he really is an ordinary Replicant after all. K hangs his head in silence; prior to that exact moment the belief that he was born instead of made was a terrifying threat to his existence, but upon discovering it was false all along, it destroyed a sense of meaning he was completely unaware he wanted — just like every other Replicant.
Freysa: You imagined it was you? Oh. You did. You did. We all wish it was us. That's why we believe!
The story for Joshi is a minor example. While she helped sustain the oppressive society, she does care for the reality and more or less treats K like a proper person, unlike Wallace's personal obsession in building his Replicant business. All of it makes her demise at Luv's hand harrowing to watch.
Joi's death. In a pure moment of Kick the Dog after kidnapping Deckard, Luv crushes Joi's emitter while K is down.
The follow-up with Joi's default avatar for ads. Despite being Ms. Fanservice, the interaction she has with K is peppered with just enough references to his version of Joi that he — and the audience — are left wondering whether his Joi loved him, or was simply programmed to fake it well. Also doubles as Trailers Always Lie, since the prominent use of nudity in the trailer is subverted by the context in the film itself.
Deckard's reaction upon seeing the Rachael clone that Wallace created as an incentive for him to cooperate. He seems shaken when he sees her face again, but then recognizes that she isn't real due to the color of her eyes. Even so, he also visibly winces when he heard Luv put a bullet in that clone's head.
Really, Rick Deckard in this movie is a pretty tragic character. Since the first movie, he lost the woman he loves to childbirth, he had to abandon his daughter at birth to protect her from people like Joshi and Wallace, who would hunt her down and retire heror worse, and he has spent the better part of the last thirty years in self-isolation in a Ghost Town. By the time K finds him in Las Vegas, he has grown even more bitter and cynical than before, because of all that he has lost.
Deckard: Sometimes, to love someone, you've got to be a stranger.
K dies of the wounds he got fighting Luv, but not before reuniting Deckard with his daughter. The fact that the 'Tears in Rain' track used during Roy Batty's final soliloquy starts playing during the scene (thus making it the only time during the film that music from the original movie is played) makes the moment all the more powerful.
Ana's situation. She is in a Gilded Cage, a glass room where she can create anything she wishes...but she can never leave it due to her condition. She states that her adoptive parents were slated to go off-world, but abandoned her when they discovered her condition. She spends her time manufacturing fake memories for Replicants, which means she works for slimeballs like Wallace, albeit as a contractor.
The brief scene with Gaff in the retirement home. Here's this, stylish, enigmatic, and unflappable blade runner grown old and infirm, dressed in drab pyjamas, and with seemingly no family to look after him when he can no longer do it himself. A sobering reminder that for all that humans live longer, they are just as vulnerable to the passage of time as replicants.
