Barkley, Shut Up and Jam: Gaiden purpots itself as a sequel to the movie: when the player talents were restored, the ball in which they were stolen\stored (referred to in-game as "Ultimate B-Ball") received residual powers and also created the Shadow Barkley that is the game's villain. The Monstars also appear in a cutscene.
CollegeHumor: A 2011 video features Lebron James in Michael Jordan's place, in which he announces he's switching over to the Monstars (referencing his controversial move from Cleveland to Miami). Ironically, this was before James was cast in Space Jam: A New Legacy.
The theme song is one of the many Running Gags on SiIvaGunner, and Charles Barkley is a supporting character in the Christmas Comeback Crisis arc (though this could be more of a reference to Barkley, Shut Up and Jam: Gaiden than anything else). In addition, a Space Jam Charity Slam was held in 2021 to coincide with the release of Space Jam: A New Legacy, with its accompanying material being modeled after the material found on the film's official website.
Animaniacs: In "Hooray for North Hollywood", Mr. Plotz lands in hot water when he turns down the Warners' movie script in favor of making Jam-A-Lot, a mix between Space Jam and Camelot.
The Fairly OddParents!: In "Space Ca-Dad", Timmy's dad says, "Let's pump up the space jam with some music!"
Foster's Home for Imaginary Friends: In "Good Wilt Hunting", a poster can be seen in the background for Astro Slam, starring Jordan Michaels and Golly Gopher. This gag also serves to foreshadow who Wilt's creator is.
Futurama: In "Time Keeps on Slipping", the Professor feeds his mutants space particles to turn them into basketball stars, spoofing the Nerdlucks' transformation into the Monstars.
In "T.H.E.Y.", Pinky's response to Brain's usual question is "I think so Brain, but I prefer Space Jelly."
In "Star Warners", two of the Monstars (Pound and Bang) make cameos in the cantina scene.
Robot Chicken: A sketch from "Catdog on a Stick" has Tiger Woods trying to salvage his reputation by making Golf Jam with DiC characters.
The Sylvester and Tweety Mysteries: In "Froggone It", Granny warns her pets not to get lost on the Warner Bros. lot, "or they'll put you in another basketball movie".
Gravity Falls: In "Soos and the Real Girl," when .GIFfany tells Soos about how she "deleted" her creators, she shows an image of a man working at a computer. The computer screen briefly flashes a binary code which, when translated, reads "SPACEJAMTWO."