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"Leonard is upstairs right now with my arch-enemy. The Dr. Doom to my Mr. Fantastic, the Dr. Octopus to my Spider-Man, the Dr. Sivana to my Captain Marvel... You know, it's amazing how many supervillains have advanced degrees. Graduate schools should do a better job of screening those people out."
Dr. Sheldon Cooper, The Big Bang Theory

"There's no scientific consensus that life is important!"
Professor Farnsworth, Futurama

Dr. John Watson: Holmes, I seem to see dimly what you are hinting at. We are only just in time to prevent some subtle and horrible crime.
Sherlock Holmes: Subtle enough and horrible enough. When a doctor does go wrong, he is the first of criminals. He has nerve and he has knowledge. Palmer and Pritchard were among the heads of their profession.
The Adventures of Sherlock Holmes, "The Adventure of the Speckled Band"

"He is a man of good birth and excellent education, endowed by nature with a phenomenal mathematical faculty. At the age of twenty-one he wrote a treatise upon the binomial theorem which has had a European vogue. On the strength of it, he won the mathematical chair at one of our smaller universities, and had, to all appearances, a most brilliant career before him. But the man had hereditary tendencies of the most diabolical kind. A criminal strain ran in his blood, which, instead of being modified, was increased and rendered infinitely more dangerous by his extraordinary mental powers. Dark rumors gathered round him in the University town, and eventually he was compelled to resign his chair and come down to London."
Sherlock Holmes on Prof. James Moriarty, "The Final Problem"

Doctor: Just one small question, ah... why do you want to blow up the world?
Zaroff: 'Why?' You, a scientist, ask me why? The achievement, my dear Doctor! The destruction of the world! The scientists' dream of supreme power!

"I don't believe that man's ever been to medical school."
Buzz, Toy Story

"We are concerned with the academic first, saving lives second."
Dr. Heinemann, Monster

Buzz: Evil Dr. Porkchop!
Hamm: That's MISTER Dr. Porkchop to you!

"Some call me mad, but I much prefer 'Dr. Cranium'."
Dr. Cranium,note  Quest for Glory IV: Shadows of Darkness

"Something terrible is about to enter our world and this building is obviously the door. The architect's name was Ivo Shandor... He was also a doctor. Performed a lot of unnecessary surgery. And then in 1920 he founded a secret society. After the First World War, Shandor decided that society was too sick to survive. And he wasn't alone: he had close to a thousand followers when he died."

"Edward Bailey was a genius physicist who built some of the greatest stuff during the Cold War: neutron bombs, particle beam weapons, nerve gas weapons — excellent nerve gas. He was a rock star of conventional mass killing. They called him the 'Da Vinci of Death'."
Marvin, Red 2

"She was the key figure in project Valhalla. When the funding was discontinued, she simply refused to quit. She knew exactly what she had in her hands."
Alfred Woden on Nicole Horne, Max Payne

"Can you believe it? Wimpy old Professor Warren — and look at me! Able to take on not one, but two Spider-Men at the same time! I'd like to see the nitwits at NSU deny me tenure now!"
The Jackal, The Clone Saga

Dr. François Duvalier was the former President for Life of Haiti, a title which he gave himself because that's the kind of thing you do when you're a crazy dictator. His father was named Duval Duvalier and was a judge while his mother, Ulyssia Abraham, was insane. The only thing missing was exposure to radiation to help him on his way to perfecting his villainy.

"I swear I'm not just saying this over bitterness over my own botched attempt to get an academic PhD, honest: I find it really pretentious when people who have non-medical doctorates go around calling themselves 'doctor.' Maybe Doc Ock wanted to call himself 'Professor Octopus' but then he got an angry letter from the provost reminding him that he was only an adjunct lecturer."

Once the massive methodological and ethical flaws in this paper came to light, the Lancet rejected it about as hard as Jehovah rejected Lucifer. Like Lucifer, the paper has spawned a vast number of evil and stupid minions bent on destroying the world.
Rational Wiki on the Wakefield paper

"Are there really a lot of people named 'Dr. Hell?'"

"Scotland Yard has top man working on the case: James Xavier, whose head appears to be emerging from a bear trap made of hair. Xavier, whose voice sounds eerily familiar, believes that the culprit behind these... crimes? is one Nathaniel Essex, but the cops refuse to believe him. See, Essex is in his sixties, but the culprit is clearly a younger man. Also, the cops are wary of charging a respected 'man of science' with these crimes, which is understandable. I mean, who ever heard of an evil scientist?"
Chris Sims on X-Men: The Animated Series ("Descent", or "No One Cares About Mister Sinister")

"I didn't spend six years at evil medical school to be called 'mister', thank you very much!"
Dr. Evil, Austin Powers

"I expect you are both hungry. Pray be patient. I will be brief. So, if you recall, there I was, in Milwaukee. In due course, I completed my studies and I left America and went by the easy stages round the world. I called myself 'doctor' because doctors receive confidences and they can ask questions without arousing suspicion."
Doctor Julius No, Dr. No

"Seems like every day there's another one, doesn't it? Today a Havoconda or Machismollusk, tomorrow a Doctor Destruction or Doctor Damage or Doc Dojo. (And do not get me started on supervillain abuse of the word 'doctor'; only a bare handful have any medical or scientific training at all, and Doctor Damage has a doctorate in Medieval English — bah!)"
SÜPER, by Corey Redekop

"These three jokers can't be real doctors, right? Last time I checked, a PhD in evil wasn't a real major."
Deadpool giving the player his opinion on Doctor Doom, Doctor Octopus, and Moonstone before the player begins the "Evil Geniuses" mission in the "Deadpool vs. Villains" event of Marvel Puzzle Quest

Don't say that he's hypocritical,
Say rather that he's apolitical.
"Once the rockets are up, who cares where they come down?
That's not my department, " says Wernher von Braun.
Tom Lehrer, "Wernher von Braun", That Was the Year That Was

"Zat was not medicine!"
The Medic, Team Fortress 2

Mr. Freeze: Soon an eternal winter will fall on Gotham and the world will know the name Dr. Freeze.
Batman: Doctor? You're Mr. Freeze.
Mr. Freeze: I'm a doctor. I went to medical school. I want to be called Dr. Freeze.
Batman: I can't allow that! If I let you go by Doctor everybody will want to. Dr. Harley Quinn, Dr. Poison Ivy, Dr. Man-Bat, Dr. Scarecrow.
Mr. Freeze: Wow, you have a lot of doctor villains.
Batman: Yeah, for such a crime-ridden city our education is best in the country. We're 80% doctors.

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