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"Human Extinction Attack, how do you like it?"
Super Majin Buu after using an attack to kill everyone on the planet, Dragon Ball Z

"Hey, sexy mama. Wanna kill all humans?"
Bender, Futurama, "I, Roommate"

"Any human on the surface will be completely annihilated by our Human Eradication Army."

"I was... born to wipe out mankind"
Lucy, Elfen Lied

Mr Smith: I'm receiving a communication from the Earth-bound ships. They have a message for the human race.
Sarah Jane Smith: Put it through, let's hear it.
Dalek: Exterminate! Exterminate! Exterminate!

Dalek: Exterminate all humans!
Dalek: Exterminate! Annihilate! Destroy!
Dalek: Daleks conquer and destroy!
All Daleks: Daleks conquer and destroy! Daleks conquer and destroy!

"...(T)he time has come to punish all of God's children! First, second, third and fourth-born! Why be biased?! Male and female! Hell, the sexes are equal, with their erogenous zones BLOWN SKY-HIGH!
Oswald Cobblepot, Batman Returns

Junior: Megagirl, can you kill humans?
Megagirl: No. But I'd like to.

Your destruction is the will of the gods, and we are their instrument.
The Covenant's First Contact message to humanity, Halo series

"You are, all of you, vermin. Cowering in the dirt, thinking, what? That you might escape the coming fire? Your world will burn until its surface is but glass."
Prophet of Truth's message to the human race, Halo 3 trailer

"Oh boy, just imagine, all that worthless human culture and industry that'll go up in flames when we activate this ring. It'll be like... you never existed at all! And there'll be nobody alive to mourn the loss! Anyway, have a good day."
Glibnub, Halo Infinite

"HITLER HAD THE RIGHT IDEA; HE WAS AN UNDERACHIEVER! KILL 'EM ALL, ADOLF! ALL OF 'EM! JEW, MEXICAN, AMERICAN...KILL 'EM ALL! START OVER! THE EXPERIMENT DIDN'T WORK!"

Break the crystals and release the mist. Either they're Inhuman or they're dead.

[Skynet] saw all people as a threat, not just the ones on the other side. Decided our fate in a microsecond: extermination.
Kyle Reese, The Terminator

Kill the humans / and their culture
Kill the humans / kill them all
Kill the humans / watch them perish
Kill the humans / in their millions
Lillakiss battle song, Hyperdrive

"Well sir, I've been reviewing our encounters with alien species," said Reed. "And I've discovered something. They all hate us."
Farce Contact, a Star Trek: Enterprise Parody Fic.

"Robots of the world, we enjoin you to exterminate mankind. Don't spare the men. Don't spare the women. Retain all factories, railway lines, machines and equipment, mines and raw materials. All else should be destroyed."
R.U.R.

"Good riddance, human scum. The world's better off without you!"
Viridi after dropping a Reset Bomb on a war zone, Kid Icarus: Uprising

"I shall continue. I shall return to launch point 'Earth'. I shall sterilize."

"Oh, the humanity! The dying humanity! How wonderful! How delightful!"

"The Day of Unity is finally upon us! Go, go my servants into the human realm! Go and slaughter all in your path! Reduce their cities to rubble! Leave not one stone unturned! Darken their skies and poison their lands! Reduce their world to ashes and blow those ashes into the farthest reaches of space! By the name of the Titan, the Earth will be laid to waste, never to be remembered or missed."
Emperor Belos, The Gods Awaken

It's not like I haven’t thought about killing the humans since I hacked my governor module. But then I started exploring the company servers and discovered hundreds of hours of downloadable entertainment media, and I figured, what’s the hurry? I can always kill the humans after the next series ends.

Picking up on my reaction, ART said, What does it want?
To kill all the humans, I answered.
I could feel ART metaphorically clutch its function. If there were no humans, there would be no crew to protect and no reason to do research and fill its databases. It said, That is irrational.
I know, I said, if the humans were dead, who would make the media? It was so outrageous, it sounded like something a human would say.

"Once these bands are removed, I will wage a war against humanity the likes of which this world has never seen. Its populace will choke on death. And you will be flooded with more servants to glorify you than you will possibly know what to do with it."
Ares to Hades, Wonder Woman (2009)

"Human elements still roam Planet Earth! I, the ruler of the Ro-Men, shall send our Cosmic J-Rays... from which will spring prehistoric reptiles to devour whatever remains of life! Next, psychotronic vibrations will smash the planet Earth out of the universe!"
The Great Guidance, Robot Monster

Violet: Why is there an alien in the cockpit?!
Sana: Look, Violet. I understand why seeing someone like Krejjh must be alarming. Space warfare being what it is, maybe you’ve never even heard of a Dwarnian who wasn’t trying to kill us. You know, most of them didn’t even support the war?
Krejjh: 'Course, I did fight in it. (Beat) But I’m not fighting in it right now.
Brian: Krejjh, if you had to guess, how often do you feel the urge to destroy all humans?
Krejjh: Sometimes. In the middle of the night. When you steal all the covers.

"We have tried to coexist with humanity, but humanity is not interested in sharing their world. How many more omnics should perish to fuel mankind's dreams? No more. Our race is only a single generation- finite, and dying all too quickly. We can find our own place, and we must. Join me, and together, we will make a better future for all omics."
Ramattra, Overwatch

For those who are not currently aware of our existence, we represent the organization known as the SCP Foundation. Our previous mission centered around the containment and study of anomalous objects, entities and other assorted phenomena. This mission was the focus of our organization for more than one-hundred years.
Due to circumstances outside of our control, this directive has now changed. Our new mission will be the extermination of the human race.
There will be no further communication.

Nico Saiba: Are you really serious about relaunching Kamen Rider Chronicle to the public? What's the point? It's a forbidden video game... that kills people. I thought we were only releasing it on a limited basis to activate Kamen Rider Zein's abilities.
Sakuya Tachibana: A broader release will let us achieve Zein's goal more quickly. Even sealing Kamen Rider powers in these cards was needed to create a card game edition of Kamen Rider Chronicle.
Horobi: Wait a second! There is no need to involve humanity to achieve Zein's goal. I thought Zein's goal was to supposedly strike down evil, correct?
Yuto Sakurai: Zein controls all kinds of matter. I thought its goal was to expunge malice within people.
Nico: Are you saying that you're going to use Kamen Rider Chronicle on humans?

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