Cassie's attempts to Dom her husband, Tom... don't go exactly as planned, made even funnier by her hammyI Have You Now, My Pretty proclamation a moment before.
Tom:*Easily breaking the ties she'd used* Sorry, my nose has been itching something fierce.
(even later) Honey, you have the knot-tying skills of a movie villain!
Although the end of chapter 4 is very serious Ally recounting how the doctor that treated her fractured wrist got a lot of mileage out of her Naughty Nurse Outfit is a much needed bit of levity.
When Lisa starts up a new erotica series to get over the break-up with Ally, she eagerly awaits the comments that would follow her new story. Instead she gets post after post of clueless fans wondering when she'll write more Lisbeth stories. The look on Lisa's face: priceless.
Well, that and the usual trolls. But the "Where's Lisbeth?" posts overwhelm the trolling by 10-to-1.
"GODDAMMIT, LISA!!! WHAT THE FUCK DOES THIS MEAN?!"
Lisa ending her last story on a cliffhanger.
The entire scene with Anne and Alan.
Anne: You know, on one side, assigning "Yakety Sax" as Ally's personalized ringtone makes it easy to know it's her. On the flipside, so not having that as my sex soundtrack!
Alan: It could be kinda fun. You know, "Yakety Sex?"
Anne: Alan! Let me put this in, as you call it, a non-negotiable form. Mercy! ...
Anne: I fucking swear, this is karma biting me on the ass for messing with Cassie.
Alan: No, that would be me! (playfully bites Anne on the ass)
Anne: WAH! ...
Alan: I could kiss you...but given the experience from last night, we know how that ends.
The completely atrocious singer that Harper hires for the party at the Crimson on New Years Eve.
Ally having two left feet:
Ally: I'm a horrible dancer!
Lisa: That's okay! It's a horrible song!
At the end of the fifth arc, showing future scenes from Lisa and Ally's relationship, we see a scene of Ally looking like a sulky teenager while Lisa orders her (for as yet unknown reasons) to talk to her mother.
Lisa: You will talk to your mother like a grown up or I will! And trust me, you don't want that!
In the prequel series Mercy, flashbacks to Ally and Alan's start of their dom/sub relationship shows that they flipped a coin to see who would be the dom(me). It goes to Alan... who suddenly gets overwhelmed by the situation and panics.
They also bicker over what the Safe Word will be, and Alan goes with "Mercy" for his. When Ally argues how cliched that one is, Alan turns on his Inner Dom and says "You will say Mercy or I will show you none," it so excites Ally that she turns on her Inner Domme and ordershim to keep going.
It also works as a bit of Foreshadowing how their relationship ends and how Lisa ends up with Ally: Ally will always be a Dominatrix at heart.
Alan finds out the college has cleared out some attic space, which he realizes he can convert into a playroom for Ally and him. The first playtime ends up with him setting up the bed covered in plastic, with Ally pouring oil all over it. She then tries to hop into the bed fully naked... only to slide right off it in a rather embarrassing manner.
Other
The April Fools one-shots. Especially the 2016 one, where Sejic goes full troll and draws Furstone, a Furry cat-themed version of the comic.
"Nothing but the classiest of humor!" All the while depicting Felisa hacking up a furball in the most embarrassing way.
The 2019 April Fools, a direct sequel's from 2018, has Hamster reviving Sejic via the use of a Pet Cemetery in order to draw furry fetish comics to Hamster's specific commands. Unfortunately, Sejic suffers flashbacks to his death, ruining Hamster's dream of millions and forcing him to reclaim Sejic's thumbs. And then it gets seriously weird...