The Spider-Gangnote Miles Morales, Peter B. Parker / Spider-Man, Gwen Stacy / Spider-Woman, Peter Parker / Spider-Man Noir, Peni Parker & SP//dr, Peter Porker / Spider-Ham
Supporting Charactersnote Jefferson Morales, Rio Morales, Aaron Davis, Peter Parker / Spider-Man I, May Parker, Mary Jane Watson-Parker, Mary Jane Watson-Parker (E-616B), Ganke Lee, Ben Parker, Fisk Family, Peter Parker / The Lizard, George Stacy, May Porker, J. Jonah Jameson (E-67)
Spider-Societynote Miguel O'Hara / Spider-Man 2099, Peter Parker / Spider-Man (E-67)
Antagonistsnote Wilson Fisk / Kingpin, Olivia Octavius / Dr. Octopus, Prowler, Lonnie Lincoln / Tombstone, Mac Gargano / Scorpion, Norman Osborn / Green Goblin