The Injustice series is based on The DCU and thus has a very large cast.
- The Insurgency/Justice Leaguenote Batman, The Flash, Green Lantern, Harley Quinn, Green Arrow (Insurgency), Deathstroke, Lex Luthor, Ares, Black Canary, Green Arrow (Another Universe), Blue Beetle, Firestorm, Catwoman, Supergirl
- One Earth Regimenote Superman, Wonder Woman, Sinestro, Solomon Grundy, Robin / Nightwing, Cyborg, Shazam, Hawkgirl, Doomsday, Raven, Black Adam, Killer Frost
- The Societynote Gorilla Grodd, Scarecrow, Poison Ivy, Cheetah, Captain Cold, Deadshot, Bane
- Othernote Aquaman, Atrocitus, Brainiac, Battle Simulator Brainiac, Doctor Fate, The Joker, Swamp Thing
- DLCnote Lobo, Batgirl, Scorpion, General Zod, Martian Manhunter, Zatanna, Arkham Knight, Batgirl (Cassandra Cain), The Flash (Wally West), Green Lantern Jessica Cruz, Killer Croc, Static, The Atom, Black Manta, Darkseid, Enchantress, Hellboy, Raiden, Red Hood, Starfire, Sub-Zero, Teenage Mutant Ninja Turtles, Bizarro, Black Lightning, The Flash (Jay Garrick), Green Lantern (John Stewart), Grid, Mr. Freeze, Power Girl, Reverse Flash, Vixen
- Main Universenote Superman, Batman, Wonder Woman, The Flash, Nightwing, Cyborg, Green Arrow, Green Lantern, Shazam, Aquaman, Hawkgirl, Raven, The Joker, Harley Quinn, Lex Luthor, Catwoman, Solomon Grundy, Deathstroke, Bane, Black Adam, Ares, Killer Frost, Sinestro, Doomsday
- Non-Playable Charactersnote Lois Lane, Jimmy Olsen, Arkham Asylum, Atom Smasher, Giganta, Stryker's Island Inmates, Trigon the Terrible, Starro the Conquerer, Mirror Master, The Atom, Galaxor, Mr. Terrific, Dick Grayson, Commissioner James Gordon, Kalibak, John Constantine, The Spectre, Mr. Mxyzptlk, Plastic Man, Teen Titans, Hawkman, Green Lantern Corps, Guy Gardner, Ganthet, Kilowog, Shao Kahn, Alfred Pennyworth, Amanda Waller, AMAZO, Animal Man, Aqualad, Athanasia al Ghul, The Atom I, "Batman", Beast Boy, Bibbo Bibbowski, Blackfire, Connor Queen, Dex-Starr, Jonathan and Martha Kent, Justice Incarnate, King Solovar, Krang, The Lords of Order, Lucius Fox, Lucy, Nubia, Ra's al Ghul, Red Robin, The Rogues, The Shredder, Steve Trevor, Suicide Squad, Talia al Ghul, Victor Zsasz