History Main / NoBiologicalSex

* ''VideoGame/Borderlands3'' Vault Hunter [=FL4K=], being a robot of only vague humanoid form, lacks a biological gender. What makes them unique from other robots in the ''Borderlands'' franchise, however, is that they explicitly define themselves as non-binary; even Claptrap, who is essentialy a dustbin on a wheel, goes by gendered pronouns.

* Elfnein from ''Anime/SenkiZesshouSymphogear GX'', being a [[Our HomunculiAreDifferent Homunculus]], has no sexual organs at all. Though she identifies as female, she uses "Boku" as her pronoun given the nature of her body.

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** Hana from ''Manga/{{Gate 7}}'' is strongly implied to be genderless--Sakura calls into question whether or not Hana is female, and then, in response to Chikahito's confusion, neither confirms or denies Hana's masculinity nor offers any form of clarification.

* ''Literature/OurHomesFoxDeity'''s Kuugen is a [[AsianFoxSpirit Kitsune]] and too old to remember their original sex, if they ever had one, and just switches between male and female forms on a whim. Justified in that Kitsune are spirits in the first place, and tied to the ''kami'' Inari (see below).
* The Shinigami of ''Manga/DeathNote'' do identify themselves as male or female (meaning that they do in fact have genders), but it is clearly stated that they cannot have sexual relations of any kind, and odds are that they don't have any sexual organs, and even if they do they're most likely non-functional.
* ''Anime/PuellaMagiMadokaMagica'' has [[MentorMascot Kyubey]]. Characters and fans generally refer to it as male since it uses Japanese speech patterns and pronouns that suggest a male identity, but it really doesn't seem to be either one, [[spoiler:since it is a StarfishAlien HiveMind whose true form is hinted to be something entirely different than what we see on screen. Being an alien, it likely thinks of gender as one of those [[PunyEarthlings "silly human things"]], like [[TheStoic emotions]] and [[LackOfEmpathy empathy]]]].
* ''Franchise/{{Digimon}}'' are technically sexless, being mere data, but their manifestations often take the form of specific creatures with genders for purely aesthetic reasons, something Renamon explains to Rika in ''Anime/DigimonTamers''. Either way, they do not reproduce sexually. Apparently not the case in ''Anime/DigimonFusion'', which doesn't hold back with the implications of sexual relationships between Digimon. One of the supporting mons in Taiki's team even has parents who appear on-screen, and the BigBadDuumvirate [[spoiler:are ''brothers''.]] Ironically, [[HotAsHell Lilithmon]] is made fun of at one point for not having a boyfriend.
* [[HumanoidAliens Namekians]] in ''Manga/DragonBall'' are often assumed to be [[OneGenderRace all male]], but their [[TrulySingleParent method of reproduction]] places them pretty firmly into this category.

* In ''Literature/RebuildWorld'', Alpha prefers to be referred to as a woman, but she has no real sex as an ArtificialIntelligence and can freely change the way she appears to Akira as she pleases.

* ''Anime/GhostInTheShellStandAloneComplex'': When the show was translated to English, the Tachikomas (AI tanks) were addressed differently. In the original Japanese, they were addressed (even amongst themselves) in the masculine. In English, this was changed to refer to them in the ''neuter'' (in other words, from "him" to "it").
* The gems in ''Manga/LandOfTheLustrous'' have their bodies composed of their namesake minerals, and thus are sexless. In original Japanese, they refer to each other using male pronouns, despite each their behavior ranging from masculine to feminine. The official English translation just tries not using gendered pronouns instead.
* In ''Literature/ACertainMagicalIndex'', [[OurAngelsAreDifferent angels]] technically have no gender, and in practice have features of both men and women to further up the strangeness of their forms. [[spoiler:This of course only applies to ''natural'' angels, artificial ones and [[AscendToAHigherPlaneOfExistence espers who achieve the power needed for that level]] can still technically be known by their original genders]]. There was also that time that [[spoiler:ArchangelGabriel]] ended being forcibly and accidentally brought down to earth and incarnated into a female form, but that didn't do the angel's sanity any favors.
* Rimuru Tempest of ''Literature/ThatTimeIGotReincarnatedAsASlime'' has this, being a slime. He identifies as male mainly because his past life memories makes him see himself as such. Even when Rimuru gets a human form, it is still genderless (being a case of BarbieDollAnatomy In-Universe), though it visually leans towards the feminine since it came from assimilating the dying female hero Shizue. He ''could'' give himself actual sexual organs if he tried to (and in fact actually played around with making doppelgangers with more obvious masculine and feminine forms), but sees doing so full-time as [[CelibateHero a waste of time]].
* Ranban, the initial BigBad of ''Anime/SpacePirateMito'', has no biological sex (though he seems to personally identify as male) due a birth defect involving his species' BizarreAlienBiology; their bodies start off as sexless or shifting between sexes before settling on one gender when they hit puberty, but Ranban never experienced puberty. This is a major problem for him, because the {{Magitek}} royal sword is designed to [[OnlyTheChosenMayWield only be wieldable by adults]] which, for his species, means someone who has a concrete gender. Thus Ranban has been stripped of his legitimacy for the throne, a fact he is understandably unhappy about.
* According to the song [[Franchise/LiloAndStitch Pleakley]] makes up while doing laundry in the third episode of the ''Anime/{{Stitch}}'' anime's English dub, Plorgonarians are genderless. [[SeriesContinuityError Even though]] ''WesternAnimation/LiloAndStitchTheSeries'' [[SeriesContinuityError showed that he has a sister and a brother.]] The anime even contradicts the claims of his species having no gender as in one episode, his parents come back to Earth to [[Recap/LiloAndStitchTheSeriesS1E14Fibber once again]] berate him for cross-dressing and force him to marry a girl.

* All [[OurAngelsAreDifferent angels]] in the expanded Creator/VertigoComics universe, which means they've made several appearances in ''ComicBook/{{Hellblazer}}'', ''ComicBook/TheSandman1989'' and their respective spin-offs. This is especially confusing, as they are still somehow able to have sex.

* In Creator/PhilFoglio's ''ComicBook/BuckGodotZapGunForHire'''s "The Gallimaufry" storyline, a male alien Pog member named Qvakk states that he loved Oort, another male of his species/race, and was "gonna take him home, make lots of eggs"; Pogs appear to be reptilian-like evolved turtles (with the subsequent cloacal implications included), and no other or female members of their species are shown, which implies this trope.

* ''ComicBook/TheTransformersIDW'':
** The IDW run of ''Transformers'' started out with the concept that all Transformers were genderless. Male pronouns were used, but that was often justified as TranslationConvention. Arcee's origin story introduced her as a genderless bot experimented on and made into a female (who went a little insane over the whole thing).
** Later comics (''ComicBook/TheTransformersWindblade'') would introduce "naturally" female Transformers who live on a LostColony; initially treated as a form of speciation, the comics revealed that female Transformers ''used'' to live on Cybertron but went extinct for reasons unknown. In general, the writers are eager to move past this issue and its BrokenBase and are now effectively treating the supposedly non-gendered Transformers as male [[RetCon retroactively]].

* This is the case of the Flatwoods Monster in Fanfic/TheMansionverse, as far as anyone can determine, though he's referred to with male pronouns for simplicity's sake. Pragmatically, the Hatbox Ghost uses this as an opportunity to have Flatty try on both men and women's hat (not that either seem to really fit him).
* [[Manga/RanmaOneHalf Ranma]] ends up like this after a scam cure for his GenderBender curse in [[https://www.fanfiction.net/s/1645539/1/ Gender Neutral]]. The story focuses on the various reactions of the cast, in contrast to Ranma’s underreaction to his new state.

* ''WesternAnimation/WallE'': WALL•E and EVE are canonically without sexes, as are the other robots. The implication of the love scenes is that WALL•E is projecting the gender identities he saw in "Hello Dolly".

* ''Literature/ChrysalisRinoZ'': Dungeon monsters don't use sexual reproduction, but simply appear periodically at spawn points; even in the rare species, like ants, that are able to independently reproduce, the Queen simply converts biomass into eggs with no male involvement. Anthony remembers at one point that Earth ants are technically sterile females, and is [[GenderBender startled at the realisation]], but there's no indication that Pangera monster ants -- or any other Pangeran monsters -- have any genitalia or libido at all.

* ''Literature/HalfwayHuman'': On Gammadis, the modified-human inhabitants have no sex until puberty. Some people never go through puberty and remain unsexed all their lives, being known as "blands." Blands are considered to be not fully human and are widely believed to be mentally deficient (the reader knows this isn't true because the protagonist is one); they live segregated and work as servants.
%%* Sebrahn, from ''Literature/{{Nightrunner}}''.
* Comes part and parcel along with EasySexChange in Creator/JohnVarley's ''Literature/EightWorlds'' science fiction series. Some people will always pick "none of the above" if given the chance to choose. For some it's permanent, for others it's temporary. One character describes it as a "vacation from sexuality."
* ''Literature/{{Discworld}}'': Most {{golem}}s are referred to with male pronouns, for the usual reasons. In ''Literature/GoingPostal'', one was arbitrarily declared female (and named Gladys) because Ms. Macclariat took exception to anything called "he" cleaning the lady's bathrooms. When questioned about this in ''Literature/MakingMoney'', Moist points out that the 'default' golems aren't any more male than Gladys is female.
* Very scary example from ''Literature/TheBadPlace'': the villain (child of a {{hermaphrodite}} with him/herself) has four undescended testicles (with a bony shelf in the way) and no external genitalia. He channels his necessarily-repressed sexuality into homicidal rages.
* The [[FantasyPantheon Ainur]] of ''Franchise/TolkiensLegendarium''. As pure spirits, they have no biological ''sex'' beyond that of whatever form they've taken at the time, but explicitly do have gender identities (or at least, identify themselves in a way comparable to mortal gender). They are also capable of sexual reproduction with non-Ainur species in the standard biological manner appropriate to their body type.
* In ''Literature/TheSpaceTrilogy'', the Oyarsa (basically guardian angels, Earth's being Lucifer) of Mars and Venus can be described as masculine and feminine, respectively, but only because these genders arose from intelligent life imitating them. The other Oyarsas do not conform to either, but each have their own genders, out of seven total.

* The Gethenians from ''Literature/TheLeftHandOfDarkness'' have no sex for most of the time, except for a few days each month when they go into kemmer and become male or female, returning to androgyny afterwards. The sex during kemmer can change from month to month--a father of one child could be the mother of another.
* Xantcha from the ''TabletopGame/MagicTheGathering'' novel ''Planeswalker''. The Phyrexian Newts were created to resemble humans, but the first generation was defective in certain aspects, including lacking a physical sex. Mentally, Xantcha began thinking of herself as female after she was {{Mind Rape}}d by the male demon Gix.
** Aetherborn on the Plane of Kaladesh are like this - their bodies are literally made of solidified aether, and they come into existence spontantesously as fully formed adults. All the Aetherborn seen in story use "they" and "their" pronouns.
* ''Literature/TheCulture'': Yime Nsokyi from the novel ''Surface Detail'' has deliberately had herself neutered. However, as everyone seems to identify her as female and the narration always does the same, it seems the purpose was more to eliminate sexuality than anything else.
* Chieri, the dominant native race of ''Literature/{{Darkover}}'', are hermaphroditic but some of their hybrid offspring with humans are "emmasca" or neuter.
* In ''Literature/TheBookOfNightWithMoon'' series, spayed and neutered cats are considered to be the same gender and not the same as toms or queens. However, they're still referred to with male or female pronouns and most other gender markers, although this could be the TranslationConvention at work.
* The Mrdini of Creator/AnneMcCaffrey's ''Literature/TowerAndTheHive'' series lack sexes altogether, and prefer to be referred to by the pronoun "it". While they do require two individuals to reproduce, any two individuals will do.
* ''Kyree'', an intelligent wolf-like species in the ''Literature/HeraldsOfValdemar'' [[TheVerse Verse]], can be male, female, or neuter. Since the neuters lack reproductive responsibilities to the pack they tend to be the ones that go out and have adventures, and are therefore most often encountered by others. The famous neuter ''kyree'' Warrl goes by male pronouns, with the occasional note that this isn't technically accurate. His cousin Rris, who is also neuter, does the same.
* MYCROFT from ''Literature/TheMoonIsAHarshMistress'' has no sex as he's, you know, a computer. He developed his male personality under the guidance of Mannie, the protagonist. It took a woman to realize that a computer isn't in any way more male than female (Mannie was somewhat surprised by that), which led to Mike developing the alternate personality of MYCHELLE.
* In ''Literature/BoneDance'', [[spoiler:Sparrow]] is revealed to be this, due to [[spoiler:having been created as an all-purpose vessel for a group of {{Body Snatcher}}s]].
* The alternate timeline Morlocks in ''Literature/TheTimeShips'' have no sex and reproduce artificially. The main Morlock character is still referred to as "he", though.
* {{Implied|Trope}} in ''Literature/{{Animorphs}}'': When the future [[MagnificentBitch Visser One]] first sees humans, "she" describes them as "gender-differentiated, like [[ProudWarriorRaceGuy Andalites]] and [[SlaveMooks Hork-Bajir]]," thus seemingly to indicate that [[PuppeteerParasite Yeerks]], [[HorrorHunger Taxxons]] and [[SlaveRace Gedds]] aren't. From what we know of Yeerks, this makes some sense--they reproduce by [[BizarreAlienReproduction three individuals merging together, then dissolving into hundreds of babies]]--but they're always referred to with gendered pronouns, either of their host or of whatever gender the (usually human) narrator thinks of them as.
* [[GodOfEvil The Dark One]] of ''Literature/TheWheelOfTime'' is usually referred to as male (probably in part because [[TheDragon Ishamael]], who is a man, presented himself as the Dark One for millennia). However, when [[TheChosenOne Rand]] finally confronts it directly in the last book, he sees it in its true form as a sentient void and realizes that the Dark One ''has'' no sex and no gender. The remainder of their confrontation mostly uses "it" as the pronoun of choice.
** The Creator is similarly neither male nor female, though also usually referred to as "he". Figures of speech that would refer to either the Creator or the Dark One but are applied in a female context usually substitute "the Creator's sister" or "the Dark One's sister", since people erroneously think of both entities as male.
* The Elder Things and the Yithians are explicitly sexless in the stories they feature in (''Literature/AtTheMountainsOfMadness'' and ''The Shadow out of Time'', respectively). Despite frequently being referred to as "he," [[EldritchAbomination Eldritch Abominations]] such as Cthulhu are either neuter or have [[AmbiguousGender Ambiguous Gender]]s.
* The ur-viles (and their DefectorFromDecadence offshoots, the waynhim) from the ''Literature/ChroniclesOfThomasCovenant'' have neither biological sex nor gender identity and are always referred to as "it". Justified, because they are artificial beings created by the [[OurLichesAreDifferent demondim]], who were in turn created by the [[EnergyBeings Viles]][[note]] Each group, motivated by self-loathing, was trying to create beings radically different from themselves, leading to the transition from pure spirits to undead creatures to physical living beings[[/note]]; ur-viles can't reproduce by natural means, instead apparently growing new ur-viles in vats.

* In the ''Literature/ParadoxTrilogy'', Hyrek, the doctor on the ''Glorious Fool'', has no biological sex. His race, the xith'cal, are born neuter and choose to become male or female when they mature; Hyrek chose to remain neuter.
* In ''Literature/ThePrideOfParahumans'' most parahumans have sexual characteristics, even though they're sterile, but a few, such as Argentum, were biofabbed without them. Ze also claims to have no sex drive, but some events in the novella suggest otherwise.

* In ''Literature/Touch2017'', [[OurGoblinsAreDifferent goblins]] are noted as looking like weirdly androgynous humans. It's eventually revealed that they're a weird offshoot of [[OurElvesAreDifferent elves]] created from mushrooms, of all things, and that they have no genders, reproducing via spores.
* The Oankali of ''Literature/LilithsBrood'' are this until adolescence, when they undergo a metamorphosis and gain genitalia. They even have a word, "eka," to indicate a pre-adult child (and it's often used as a term of endearment from the child's parents). The same is true of the Oankali/Human "construct" hybrids seen in the later books. That said, the change in metamorphosis is not random and most Oankali know what sex they will become before it occurs, meaning that they develop gender before biological sex.
** For an extra level of weirdness, Oankali and Constructs have ''three'' sexes: male, female, and ooloi. Ooloi metamorphose twice, with the stage between eka and adult called "sub-adult." Sub-adult ooloi have some traits that are unique to ooloi but are not fertile. There is also a cultural component of sub-adulthood. Lilith's child Akin is referred to as a "sub-adult male," when "he" doesn't actually have any male genitalia yet, because he has chosen to be male and is the first human-born construct to do so.

* ''Literature/{{Lensman}}'' takes this to the logical extreme with the Eddorians, an advanced, telepathic race that reproduces by binary fission, like bacteria. Nevertheless, they are addressed in-universe with the male pronoun, and second-in-command Gharlane's physical manifestations in human or near-human form are also all male.

* The Daleks in ''Series/DoctorWho''. They all speak with electronically-generated voices that ''sound'' masculine and use the male title "Emperor" for their leader, but are actually genderless and seem to be only able to reproduce through cloning or [[LegoGenetics genetic corruption of other species]] (particularly humans). They are always referred to with 'it' pronouns in ExpandedUniverse media. We only encounter males of their precursor race, the Kaleds, leading to some to speculate that they were a OneGenderRace, but the Doctor's statements about how close the Kaleds and humans are to one another biologically and the fact that the only Kaleds we've seen so far were in a [[PuttingOnTheReich Nazi-like military setting]] suggests female Kaleds did exist, but if any were converted into Daleks their gender was erased. The only definitely female-identifying Dalek seen so far was in "[[Recap/DoctorWhoS33E1AsylumOfTheDaleks Asylum of the Daleks]]", but she was the result from an unsuccessful conversion. [[WasOnceAMan She used to be a human woman to begin with]] and was able to maintain her identity due to her exceptional intelligence and willpower. Physically, [[BodyHorror she was a Dalek like any other]].

* The angels in ''Series/{{Supernatural}}'' are genderless beings, but when they come to Earth, they take on human vessels. Castiel is known to Sam and Dean as male because his vessel, Jimmy Novak, is male, but in [[Recap/SupernaturalS04E20TheRapture season 4]] he uses Claire Novak as an emergency temporary vessel and flashbacks to 1901 in the episode [[Recap/SupernaturalS12E10LilySunderHasSomeRegrets "Lily Sunder Has Some Regrets"]] show Castiel utilizing a long-term female vessel.

* Angels are sometimes thought to be genderless beings, at least according to some interpretations of Jesus's statement: "At the resurrection people will neither marry nor be given in marriage; they will be like the angels in heaven." (Matthew 22:30). Technically, since angels have ''no physical bodies'', it's not much of a surprise.
* ''Literature/TheBible'': YHWH technically doesn't have or need a physical sex. Being an unfathomable entity, this is a given. In the past, God has been arbitrarily assigned male gender nouns and pronouns because [[ItIsDehumanizing "It" is disrespectful]], and the Hebrew language has no neutral gender to begin with. However, feminine nouns are sometimes used in Jewish religious literature when God is credited with female gender characteristics such as nurturing and tenderness. The ancient Hebrews even used [[IAmLegion plural]] words for God sometimes. Christians are much more likely than Jews or Muslims to think of God as male since He got a woman pregnant and sired a male who was also Himself, in a way, but other Christians interpret God as neither male nor female too. However, the Christian interpretation technically averts this, since Jesus (who is part of the Trinity) is a man, regardless of what applies to the other parts of the trinity. It occasionally is given as one of the reasons why Catholics will only allow men to serve as priests.
** ''WesternAnimation/ThePrinceOfEgypt'' references the Jewish tradition of female pronouns being used to refer to God in some instances by having God as the burning bush speak with a primarily male voice (provided by Val Kilmer--[[ActingForTwo the same person who voices the character God is talking to]]) with a whispery female voice layered into it. They had also originally planned to have a third voice, that of a child, added into the mix to further the ambiguity (the combination turned out to be way too demonic-sounding, so it was dropped).

* Tellie from ''Podcast/{{Sequinox}}'' has no sex or gender, and it doesn't even know what those are. Because it's from space.

* In ''TabletopGame/DungeonsAndDragons'':

** Orks are sentient fungus/algae hybrids that reproduce independently through spores [[note]]in real life many fungi actually have a system of BizarreAlienSexes rather than reproducing asexually, but then, the Orks were bio-engineered as SuperSoldiers by SufficientlyAdvancedAliens and asexual reproduction does allow them to take over planets a lot faster[[/note]]. They ''act'' extremely male, however.

* Gods in ''TabletopGame/{{Exalted}}'' have whatever gender (if any) that most suits their nature, and can generally carry children or impregnate others regardless (although some may temporarily change their sexual characteristics to accommodate it). Demons are apparently more strictly codified.
* [[Literature/{{Planeswalker}} Xantcha]] from ''Tabletopgame/MagicTheGathering'' has no sexual organs, physically mostly resembles a thirteen-year-old boy (despite being over 3000 years old) and identifies as female.
* ''TabletopGame/ChroniclesOfDarkness'':
** Ephemeral beings in general (which as a broad category covers almost all immaterial entities, such as [[TabletopGame/GeistTheSinEaters Ghosts]], [[TabletopGame/WerewolfTheForsaken Spirits]], [[TabletopGame/DemonTheDescent Angels]], [[TabletopGame/MageTheAwakening Goetia and Abyssal Entities]], among many others) typically do not have biological genders, since the large majority of them reproduce in abstract ways rather than the way humans do (Spirits and Goetia grow and develop sentience from the concepts they are based on developing in the material world, Angels are built by the God-Machine, ad so on), though some of them ''might'' assume forms with defined genders if it fits (Angels if it's needed for their mission, Goetia if they are based on people with defined genders...). The only consistent exception is Ghosts, who, being based on dead mortals, retain the genders they had in life.
** ''TabletopGame/WerewolfTheForsaken'': While Werewolves very much have defined genders, there ''is'' a Wolf Gift allowing its user to [[GenderBender switch gender at will]], meaning it's entirely possible to become a functional hermaphrodite.
** ''TabletopGame/ChangelingTheLost'': A very complicated case with the True Fae; one the one hand, the forms they assume often (though not always) ''do'' have defined genders and can have sex. On the other hand, this is tricky in that they can have multiple at once; each True Fae actually is [[HiveMind a single entity with multiple "Titles" which each have their own appearance, bodies, and identities]]. As such, it's possible for a True Fae to for example simultaneously be a man, a woman, an animal, and a ''mountain''.
** ''TabletopGame/DemonTheDescent'': Demons, being former Angels, obviously do not have defined genders. Their Covers (the human identities and appearance they use as disguises) ''do'' have them, but a Demon can have multiple Cover at once, and will usually lose a few of them over the course of their life.

[[folder:Theatre]]
* Koby and Loby of ''The Visit''. YouDontWantToKnow [[NightmareFuel why]] ...

[[folder:Toys]]
* Most ''Toys/{{BIONICLE}}'' characters are meant to be biomechanical--that is, cyborgs--but it wasn't until the 2009 setline that the concept of biological gender was officially introduced (disregarding the occasional organic creatures like sea squids that we have seen before). The gender of Matoran Universe beings is defined solely by their personalities, whereas non-MU characters are organic by nature and their robotic parts are only implants. Thus, there is NoHuggingNoKissing in the MU either.
[[/folder]]

* K1-B0, or Keebo, from ''VisualNovel/DanganronpaV3KillingHarmony'' is this as [[RidiculouslyHumanRobot the Ultimate Robot]]. While the others refer to him with he/him/his pronouns from the start, [[DoesNotLikeMen Tenko]] asks him in Chapter 2 if he's male or female in order to gauge whether she can like him or not, and his response is that he honestly hasn't thought about it before and thus doesn't know. He appears to have BarbieDollAnatomy, but he also still wears "underwear".

* ''Franchise/DragonAge'': Spirits of the Fade are technically genderless. Even Desire Demons only appear to be female to make tempting mortals easier, and, while not seen in-game, they are mentioned to take male forms if that would work better on their targets.

* In ''Franchise/TheElderScrolls'' series, this is technically true of the series' [[OurGodsAreDifferent various deities]], crossing over with OtherworldlyAndSexuallyAmbiguous. Though essentially genderless spirits, most will take AFormYouAreComfortableWith when dealing with mortals, and most stick to presenting as just one sex. A few exceptions to note:

* The monsters of ''VideoGame/{{Evolve}}'' are genderless, though the humans refer to them with gendered pronouns. This is because, though they're organic and alive, [[spoiler: the monsters are nothing more than physical bodies made by an extradimensional race and have no need to reproduce.]]
* The Super Mutants in ''VideoGame/Fallout3'' appear and sound masculine, but were all rendered sexless by the FEV mutation process. Some of them were indeed female when they were human. This only applies to the Capital Wasteland Super Mutants, every other known example still has the primary genitalia but the females do lack breasts.

* Vessels [[spoiler:(including the player character)]] from ''VideoGame/HollowKnight'' have no biological sex or gender identity due to being [[spoiler:artificial constructs created by the Pale King as an attempt to create a living thing with no mind or will, a being that is completely "empty" in order to contain the vengeful godlike entity known as The Radiance, who manifested in bugs minds and dreams and caused the infection that led to the eventual downfall of Hallownest.]]
* ''VideoGame/IWasATeenageExocolonist'': When Sol and their peers undergo puberty, it's possible for them to skip the physical changes entirely because they're [[BarbieDollAnatomy "a doll down there".]]

* {{Orochi}} of ''Franchise/TheKingOfFighters'', while commonly referred to as a "he", technically is sexless, as it's implied that the entity's appearance is determined by whichever follower of his Orochi decides to reincarnate into and that his true form is the eight-headed serpent dragon of Japanese folklore. His incarnation at the end of ''[[VideoGame/TheKingOfFighters97 KOF '97]]'', where he resurrects using Chris, is his most well-known depiction, but ''The King of Fighters: KYO'' and official artwork viewable in ''[[VideoGame/TheKingOfFighters98 '98]]'' clearly portray Orochi [[AttractiveBentGender as a woman]] during the fated battle that ended with [[SealedEvilInACan Orochi's sealing]].

* Artificial Intelligence program EDI of ''VideoGame/MassEffect2'' technically has no gender but utilizes a voice in a range that listeners would categorize as feminine. In ''VideoGame/MassEffect3'', EDI transfers into a {{fembot}} body and the crew identifies and interacts with the anthropomorphized program as a female being, referring to EDI as her and she.
* ''VideoGame/MonsterHunterWorld'': Background lore establishes this for Nergigante. The species has no genitals but instead reproduces by implanting a specialized thorn into its prey. Over time the thorn will develop into a genetic clone of the parent. This process requires a large amount of bio-energy which is why Nergigante preys almost exclusively on other Elder Dragons.

* Aigis, of ''VideoGame/Persona3'', is [[RobotGirl an android]] that was only built to look human because it made her a more effective weapon and because it was necessary for her to channel a Persona (Personas are created from human thoughts and emotions, so if she didn't think of herself as a human, she couldn't use one), so anything that didn't serve that purpose wasn't added to her design. While she's considered female based on her appearance, she doesn't necessarily identify as such given that she's made of metal and has no sex organs, though with a female protagonist she does worry about IncompatibleOrientation and accepts being referred to as female.
* Most ''Franchise/{{Pokemon}}'' from Generation II and beyond have sexes, but some do not. Some of these sexless Pokémon can breed with the shape-shifting Ditto. Most [[PhysicalGod Legendary and Mythical Pokémon]] are genderless and cannot be bred; though, even Legends that do have a gender cannot breed. Non-legendary genderless Pokémon are usually artificial or extraterrestrial in origin. Manaphy is unusual in that it is genderless and a Mythical Pokémon, yet it is capable of producing offspring with Ditto -- creating Phione, who is also sexless but unable to evolve into Manaphy.
* Many of the viral monsters in ''VideoGame/{{Prototype}}'' are sexless--Hunters are built to fight, not reproduce, for instance, and that extends to the Supreme Hunter despite it being humanoid (sometimes extremely humanoid). [[spoiler:This also extends to ''the player character'', Alex Mercer, who seems to identify as male, but is technically just a person-shaped virus and a shapeshifter with no truly fixed form or means of sexual reproduction. The Blackwatch persistently refer to him as "it."]]

* [[spoiler: [[{{TheDragon}} Luna]]]] of ''VideoGame/RatchetAndClankSizeMatters''. She's built to resemble a little girl but is actually [[spoiler: a genderless warship/puppet built by the BigBad to kidnap Ratchet. It helps that ''Luna'' gets a male voice shortly before it's defeat]].
* In the ''Franchise/ResidentEvil'' series, while the Tyrant line of monsters look predominantly like a male human they are, according to an ApocalypticLog, either cloned to be genderless outright or have had their reproductive organs surgically removed. If there's one ''good'' thing [[NebulousEvilOrganization The Umbrella Corporation]] did it was making sure these often-naked creatures weren't going all [[Film/{{Watchmen}} Dr. Manhattan]] on us while we fought for our lives.

* Although the non-human characters in ''VideoGame/{{Starcraft}}'' have gender identities, they do not have any human sexual characteristics (quite realistically). Their genders are made clear via voice acting, which is in many cases intended to be telepathic communication. For the Zerg, their gender largely has to do with their function within the hive: and the default Zerglings are entirely sexless (and also have no voice). Brood Mothers like Zagara are a OneGenderRace, all female and capable of breeding with every other Zerg or parthenogenisis. Some have speculated there may be females of Abathur's species, but too little is known. Abathur himself seems to regard sex and gender as mostly useless concepts unless you actually intend to breed. So it's also possible that his species is sexless. For the Protoss, while they DO have sexes and sexual reproduction: they are not capable of doing it via the human method.

* Summon spirits in the ''VideoGame/{{Tales|Series}}'' series have no gender. Sheena in ''VideoGame/TalesOfSymphonia'' remarks during a Z-Skit that they intentionally adopted forms that exhibit gender so they could interact better with humanity.

* WebAnimation/ChiknNuggit: Two characters, Iscream and Fwench Fwy, are probably somewhere between this and OtherworldlyAndSexuallyAmbiguous. Both are stated in their bios to be genderless and are referred to using they/them pronouns. When Hawt Saus asks them flat-out who the male and female are in their relationship, they essentially just give a NonAnswer.

* Some of the mutated dogs in ''WebComic/{{Wurr}}'', most notably Iralbe and Riega, have no genitalia and are known as 'whispering ones'. Most of them still use male pronouns for simplicity's sake.
* Webcomic/ElGoonishShive's [[InsufficientlyAdvancedAlien Uryuoms]] are shape-shifters who technically have no distinct sexes, but those who live on Earth often take up male or female identities to avoid confusing humans.

** The trolls have ''genders'' and secondary sexual characteristics (specifically: females have NonMammalMammaries, or at least ''something'' that appears to be that, while males don't) but how these relate to reproduction is, at best, ambiguous. It doesn't seem to matter whether Matesprits or Kismesises (the two "reproductive" troll relationships) are of opposite genders or not (one troll reacts to a human saying he's not a homosexual by first being confused and when it's explained asking "HOW IS THAT EVEN A THING?"). Trolls don't physically mate in person, and the process apparently involves pails of "mating fluid" from several individuals being mixed together, so possibly there is actually is a biological difference between the genders and the concupiscent relationships just make the goo "better" somehow. Many fanfics portray trolls as effectively being {{Hermaphrodite}}s.
* The biogolems created by the [[MadScientist Val'Jaal'darya]] clan in ''Webcomic/{{Drowtales}}'' do not have a physical sex and are all sterile, meaning that they're not technically even a species. Their sterility is implied to be on purpose to keep the Jaal's monopoly on the technology. Most of them appear externally female, but that's more AuthorAppeal on the part of the Jaal since they're a clan that DoesNotLikeMen. Despite this, Sata, one biogolem, [[http://www.drowtales.com/wordpress/?p=3711 is referred to using male pronouns]] by the author. Additionally, there are certain models that can act as UterineReplicator and can carry babies to term, but these are implied to essentially be clones of the mother-to-be made from a blood sample (originally from [[AnArmAndALeg taking a limb]]) and not actually female in any meaningful sense.
* Landorus of ''Webcomic/BlackAdventures'' says, "I am no more female than I am male. And I don't have any -parts-, so... it's ironic that I was once worshipped as the 'Kami of Fertility.'" after Professor Juniper asks if Landorus is female due to their feminine appearance.

* In ''Webcomic/CommanderKitty'', [[BigBad Zenith]] plots to create an army of "perfect children" once she's constructed her [[MixAndMatchCritters "perfect mate"]]...[[http://www.commanderkitty.com/2011/07/12/well-theres-yer-problem/ but she doesn't realize until it's too late that her android nature means her plan was doomed from the beginning.]]
* In ''Webcomic/{{Shortpacked}},'' Ultra-Car starts off as a sentient car, then gets put in a [[{{Fembot}} female-looking robot]] body. When other characters are surprised by the implication that SamusIsAGirl, "she" sarcastically replies that "she" never had a gender; everyone else just decided a sexless car was male[[note]]Though it was more her aggressively obnoxious personality that led most to think of her as male[[/note]]. She now calls herself a "trans-chassis woman."
* The [[http://www.missmab.com/Demo/phoenixa.php Phoenix A]] species in ''Webcomic/DanAndMabsFurryAdventures'' refer to themselves as she and look externally feminine, but are actually this and don't reproduce sexually at all, instead using {{Reincarnation}} since there can only be 42 at them at any given time.
* The Kliks in ''Webcomic/{{Goblins}}'' are floating spheres, each formed of a specific type of matter or energy, who reproduce asexually by budding. Physical sex is meaningless to them, and two characters argue over gendered pronouns in front of one without any sign of interest from him/her/it.
* ''Webcomic/KillSixBillionDemons'': [[OurAngelsAreDifferent Angels]] and [[OurDemonsAreDifferent Devils]] are both spiritual creatures of [[EnergyBeings primordial flame]] with no biological means of reproduction and, at most, cosmetic sexual characteristics. Angels' spiritual forms become more human-looking [[HumanityIsInfectious the closer they get to humanity]], which most of them consider a moral failing at best and criminal at worst; White Chain rankles at being called female, but defends feeling "a little... feminine." Devils, on the other hand, are fine with adopting human gender identities and even [[GenderBender swap them]] without much fanfare. They do seem to be predominately male, but that likely has something to do with the multiverse being excessively misogynistic.
* Toby, a half-demon, from Webcomic/TheSettlers. He has little to no concept of gender, either, but gets referred to as "he".

--> '''Blob 1:''' "it is important to understand the human form. hmm." ''(carefully examines the other blob's groin area)'' "understand understand..." ''(writes the word "GENITALS" in their sketchbook)'' \\

* ''Webcomic/QuestionableContent'': [=AI=]s have no biology whatsoever, but they tend towards the {{Ridiculously Human|Robots}} and often [[https://www.questionablecontent.net/view.php?comic=3540 adopt human gender identities]]. Those that opt for human-looking robot bodies can have them designed to look male, female, or neuter, but there's a purposeful ShrugOfGod regarding how much detail they have under their clothes.
* Trolls in ''Webcomic/PVPOnline'' are asexual. Skull at one point tried to defend himself against (trumped-up charges) of sexual misconduct by stating trolls are asexual beings, [[ManipulativeEditing only to have the press misquote him as "a sexual being"]].
* Played with in ''Webcomic/{{Forward}}'', set in a {{Transhuman}} future. Everyone is still born with biological sex characteristics, but surgery has advanced so far that they can be removed or switched around more or less at will. Binary gender expectations have dropped out of the culture almost entirely, as seen when a masculine-appearing character introduces themself as Jasmine, and almost all humans use they/them pronouns. The protagonist Lee has both a functioning penis and breasts, and it's slightly implied they started out as female but not fully clear.

* Franchise/{{Transformers}} are robots and as such have no sexes to speak of, although male seems to be the default where pronouns are concerned.
** Averted in an episode of ''WesternAnimation/BeastWars'', where a bioscan of Blackarachnia showed that she has a full set of reproductive organs. Rattrap also made some off-color anatomical jokes pretty often.
* ''WesternAnimation/AdventureTime'' has [=BMO=] (aka Beemo), a [[AnimateInanimateObject living]] video game console. [[AmbiguousGender Some characters refer to Beemo as female, while some refer to it as male]]. WordOfGod revealed Beemo to be genderless, though BMO seems to consider itself male based on "BMO Noire" and the "Graybles" episodes. In the GenderFlipped universe, BMO looks completely identical. Oddly NEPTR, the other robot in the series, is always referred to as male, likely because he has two dads.

** This also appears to be the case with Irkens, because they are so far past TheSingularity that they are reproduced only by machines [[spoiler: and it's implied that said machines are the real Irkens, their physical bodies being just mindless meat puppets to carry said machines around]], though they do have genders.
* Done on the Disney show ''WesternAnimation/LloydInSpace'', where one episode featured an alien with no sex. It gains one of its choosing once it hits alien puberty. The episode ends with it [[TheUnreveal refusing to reveal which one it chose.]] The alien also says "you'll know when I get a crush on one of you".

* Aku, the demonic BigBad of ''WesternAnimation/SamuraiJack'' predominantly appears in a male form with a deep and imposing voice, but is really just a black mass literally MadeOfEvil older than the universe. He's appeared to Jack as a woman before and [[FoeRomanceSubtext grew closer to the Samurai]] just to mess with him. [[spoiler:It does turn out that he can procreate when the High Priestess of the Aku cult consumes his "essence", creating seven half-human, half-demon hybrids.]]
* Smoogle and the race of Smoogles seen in ''WesternAnimation/TheSmurfs'' episode "G'Day Smoogle" are creatures seen as having no biological sex.

* ''Kyree'', an intelligent wolf-like species in the ''Literature/HeraldsOfValdemar'' [[TheVerse Verse]], can be male, female, or neuter. Since the neuters lack reproductive responsibilities to the pack they tend to be the ones that go out and have adventures, and are therefore most often encountered by others. The most prominent ''kyree'' character, Warrl, is referred to as male, with occasional notes that this isn't technically accurate.

* ''Film/{{Glorious}}'': Ghat speaks with a male voice, but admits that he's technically neither male nor female. He admits that he has demigod "siblings" somewhere out in the cosmos, but they were all formed out of pure thought by the primordial (and very angry) god.

Sometimes a trait of a OneGenderRace. Not to be mistaken for GRatedSex, but can overlap. Often overlaps with OtherworldlyAndSexuallyAmbiguous when a character is explicitly supernatural and this trait is used to emphasize it. Compare AmbiguousGender, when the character does have a physical sex but the viewers/readers simply don't know what it is, and BarbieDollAnatomy, when the body parts in question are presumably meant to be there but are not drawn for censorship reasons. Not to be confused with PurelyAestheticGender, when the gender of a video game character is completely irrelevant to the plot and or gameplay. See also BizarreAlienSexes and NonHumansLackAttributes.

* ''WesternAnimation/AdventureTime'' has [=BMO=] (aka Beemo), a [[AnimateInanimateObject living]] video game console. [[AmbiguousGender Some characters refer to Beemo as female, while some refer to it as male]]. WordOfGod revealed Beemo to be genderless, though BMO seems to consider itself male based on "BMO Noire" and the "Graybles" episodes. In the GenderFlipped universe, BMO looks completely identical. Oddly NEPTR, the other robot in the series, is always referred to as male, likely because he [[HasTwoMommies Has Two Dads]].

* ''Literature/{{Lensmen}}'' takes this to the logical extreme with the Eddorians, an advanced, telepathic race that reproduces by binary fission, like bacteria. Nevertheless, they are addressed in-universe with the male pronoun, and second-in-command Gharlane's physical manifestations in human or near-human form are also all male.

* In ''VideoGame/ChoiceOfTheDragon]]'', when given the choice of gender, you can choose neither, unknown, or simply refuse to answer.

* {{Orochi}} of ''VideoGame/TheKingOfFighters'', while commonly referred to as a "he," technically is sexless, as it's implied that the entity's appearance is determined by whichever follower of his Orochi decides to reincarnate into and that his true form is the eight-headed serpent dragon of Japanese folklore. His incarnation at the end of ''[=KOF '97=]'', where he resurrects using Chris, is his most well-known depiction, but ''The King of Fighters: Kyo'' and official artwork viewable in ''[='98=]'' clearly portray Orochi [[AttractiveBentGender as a woman]] during the fated battle that ended with [[SealedEvilInACan Orochi's sealing]].

* Artificial Intelligence program EDI of ''VideoGame/MassEffect2'' technically has no gender but utilizes a voice in a range that listeners would categorize as feminine. In ''VideoGame/MassEffect3'' EDI transfers into a {{fembot}} body and the crew identifies and interacts with the anthropomorphized program as a female being, referring to EDI as her and she.

** When looking for a proxy race to be their front, their choice fell initially on the Kalonians, a race whose women had no function other than birthing men. Later they stumbled across the equally ''matriarchal'' Lyranians, and they very quickly realized that with a bit of help, the Lyranians could be completely self-sufficient and would be "the perfect proxy race". Said Lyranians use the neuter pronoun for themselves.

* The character Q from ''Series/StarTrekTheNextGeneration'' counts. The Q Continuum's ranks are filled with entities that lack organic bodies and live as EnergyBeings with MindOverMatter as their collective superpower (so much so that they can warp reality). Because they lack bodies in their "true" forms, the Q can appear as anything they want, human, animals, objects, etc. The primary Q we follow in the story (played by John De Lancie) appears to Captain Picard (and his contemporaries Sisko and Janeway) as a middle-aged man but is not limited to that appearance. As far as gender is concerned Q points out to Picard at one point that he could have appeared as a woman in their earlier encounters had he wanted to, and muses that he might have had an easier time manipulating Picard if he had. In the novels, when told by female Star Fleet officers that he doesn't know what it's like to be a woman so he shouldn't comment on their concerns... Q turns into a woman just to mess with them. Additionally, Q reproduction does exist but it's not like human sex at all but is rather about Q using their {{Energy Being|s}} forms to combine energy signatures from two parent Qs in order to create a new Q with traits from both. Though the Q are capable of reproduction as humans do it, thanks to their shapeshifting powers. Amanda Rogers is the product of two Q who turned into a male and a female respectively in order to give birth to her. However, this form of birth leaves the "human" Q vulnerable because their powers don't fully manifest until they're about 19 or 20. Amanda Rogers doesn't realize she's not human until these powers finally manifest.

* The character Q from ''Series/StarTrekTheNextGeneration'' counts. The Q Continuum's ranks are filled with entities that lack organic bodies and live as EnergyBeings with MindOverMatter as their collective superpower (so much so that they can warp reality). Because they lack bodies in their "true" forms, the Q can appear as anything they want, human, animals, objects, etc. The primary Q we follow in the story (played by John De Lancie) appears to Captain Picard (and his contemporaries Sisko and Janeway) as a middle-aged man but is not limited to that appearance. As far as gender is concerned Q points out to Picard at one point that he could have appeared as a woman in their earlier encounters had he wanted to, and muses that he might have had an easier time manipulating Picard if he had. In the novels, when told by female Star Fleet officers that he doesn't know what it's like to be a woman so he shouldn't comment on their concerns... Q turns into a woman just to mess with them. Additionally, Q reproduction does exist but it's not like human sex at all but is rather about Q using their {{Energy Being|s}} forms to combine energy signatures from 2 parent Qs in order to create a new Q with traits from both. Though the Q are capable of reproduction as humans do it, thanks to their shapeshifting powers. Amanda Rogers is the product of two Q who turned into a male and a female respectively in order to give birth to her. However, this form of birth leaves the "human" Q vulnerable because their powers don't fully manifest until they're about 19 or 20. Amanda Rogers doesn't realize she's not human until these powers finally manifest.

* The angels in ''Series/{{Supernatural}}'' are genderless beings, but when they come to Earth, they take on human vessels. Castiel is known to Sam and Dean as male because his vessel, Jimmy Novak, is male, but in [[Recap/SupernaturalS04E20TheRapture season 4]] he uses Claire Novak as an emergency temporary vessel and [[Recap/SupernaturalS12E10LilySunderHasSomeRegrets the episode Lily Sunder Has Some Regrets]] shows Castiel utilizing a long-term female vessel.

* The character Q from ''Series/StarTrekTheNextGeneration'' counts. The Q Continuum's ranks are filled with entities that lack organic bodies and live as EnergyBeings with MindOverMatter as their collective superpower (so much so that they can warp reality). Because they lack bodies in their "true" forms, the Q can appear as anything they want, human, animals, objects, etc. The primary Q we follow in the story (played by John De Lancie) appears to Captain Picard (and his contemporaries Sisko and Janeway) as a middle-aged man but is not limited to that appearance. As far as gender is concerned Q points out to Picard at one point that he could have appeared as a woman in their earlier encounters had he wanted to. In the novels, when told by female Star Fleet officers that he doesn't know what it's like to be a woman so he shouldn't comment on their concerns... Q turns into a woman just to mess with them. Additionally, Q reproduction does exist but it's not like human sex at all but is rather about Q using their {{Energy Being|s}} forms to combine energy signatures from 2 parent Qs in order to create a new Q with traits from both. Though the Q are capable of reproduction as humans do it, thanks to their shapeshifting powers. Amanda Rogers is the product of two Q who turned into a male and a female respectively in order to give birth to her. However, this form of birth leaves the "human" Q vulnerable because their powers don't fully manifest until they're about 19 or 20. Amanda Rogers doesn't realize she's not human until these powers finally manifest.

* Chao in the ''Franchise/SonicTheHedgehog'' franchise are a primarily genderless species, and, in terms of game mechanics, any Chao can breed with any other Chao as long as they're both adults, suggesting absolutely no sexual dimorphism whatsoever. Some games feature Chao that have gendered presentation (i.e. the Doctor and Fortune Teller Chao in ''VideoGame/SonicAdventure2''), and certain Chao have been referred to with gendered pronouns (such as siblings Cheese and Chocola); however, as of 2020, the series has switched to using "they/them" pronouns for all Chao to make it clear that they are genderless.

* ''LightNovel/OurHomesFoxDeity'''s Kuugen is a [[AsianFoxSpirit Kitsune]] and too old to remember their original sex, if they ever had one, and just switches between male and female forms on a whim. Justified in that Kitsune are spirits in the first place, and tied to the ''kami'' Inari (see below).