WARNING: Spoilers are unmarked.
- During the opening, Daphne mentions in an interview that even though she, Fred, Velma, Shaggy, and Scooby ultimately got tired of solving Scooby Doo Hoaxes and (for the most part) went their separate ways, she and Fred still miss Velma, Shaggy, and Scooby. While currently working as airport security personnel, Shaggy and Scooby happen to witness this while on-the-job, and admit to feeling the same way.
- On that very topic, Shaggy and Scooby wind up losing their job due to eating all the contraband... and Shaggy implies that this isn't the only time something like this happened to them. Luckily, Fred calls them almost immediately after.
- Velma's bookstore doesn't appear to be doing well either, which is Truth in Television: A lot of small bookstores were going out of business at the time this film was produced due to online competition.
- Turns out that the zombies are not the bad guys; their souls just couldn't rest until the real monsters were finally brought to justice and the life-force that was consumed from them was freed.
- All of the initial paranormal activity, such as ghosts appearing in mirrors, writing threatening messages on the wall and even Morgan Moonscar himself coming back from the dead was actually the spirits of long dead victims trying their absolute best to get the Scooby Gang out of there. Not only can they not communicate effectively, that's exactly what Mystery Incorporated went to this swamp for, so of course they're going to stay. In the end, the dead are powerless to change the fates of the living.
- Special consideration should be paid to the original set of zombies, Morgan Moonscar and his pirate crew. Though they were truly monstrous in life, they're trying just as hard as the rest of the zombies to protect people from sharing in their fate. Considering the zombies are fully aware of their situation, it's not much of a leap of logic to think that Moonscar and his crew have had two entire centuries to realize what monsters they were in life and that others must be protected from the Werecats.
- Even aside from the pirates, just looking at the other people that have fallen victim to Lena and Simone's machinations. The people who sought out the bayou as a place to build a plantation home, only to be killed and have their achievements and their crops stolen by cat women. The soldiers who may have simply been unlucky enough to pass through who would be considered just a few more nameless, lost, unknown casualties in the Civil War without any word to their families as to what happened to them. And even if these aforementioned people weren't morally pure (given what a plantation in the South usually entailed at the time and the fact many of the soldiers were Confederates), you only have to look at the modern zombies. Normal, everyday tourists who sought only to see the sights on what could have been time off from work, a trip to new places, or even trying to do something with a loved one.
- As the zombies crumble to dust, the spirit of Jackson Pettigrew, a Confederate soldier, is shown saluting Scooby before he disappears, either into the afterlife or just into oblivion. Anything is better than his former wretched existence.
- Let's not forget, as the above example implied, that plantation almost certainly would have had slaves working on it. At least the settlers who started the plantation presumably wanted to be there initially. How many of Simone and Lena's victims were trapped on Moonscar Island by force and then had to haunt it for over a century after they were killed?
- Then there's Simone and Lena's Dark and Troubled Past, where they were the Sole Survivors of their group of settlers who had originally settled the island after Morgan Moonscar had them all massacred. It absolutely does not justify their actions in the centuries that followed, but being forced to watch everyone you knew and loved be murdered without a second thought by a band of pirates who thought nothing of taking lives purely for the sake of it, and being absolutely helpless to stop it is more than enough to drive anybody over the edge. And, of course, the realization that they'd been cursed themselves makes things more depressing to the eye.
- Though the gang manages to survive, they're clearly very shaken by the ordeal. When Simone revealed their true nature, Daphne worriedly whimpered that this was more than she bargained for, and it's not hard to imagine Daphne felt guilty about nearly getting everyone killed just for her show and desire for real monsters. The gang didn't even survive by working together as a team to pull of a scheme like they usually do - it was sheer dumb luck that bought them enough time for the werecats' deadline to arrive. They were being cornered by the werecats when time was up, and Scooby and Shaggy already nearly got drained during the climax.
