Tear Jerker / Monty Python's Life of Brian

• The bits between the botched (or successful, depending on your outlook) Suicide Squad raid and the finale. Brian is being crucified, and not even those he loves are going to help him. His lover takes his "martyrdom" with pride, and his mother berates him and leaves him for dead.
  Brian: Mum... Mum!
• Though the rest of the scene is Played for Laughs, Brian's/the crowd's interactions with the hermit end on a genuinely sad note, with the hermit being labelled a heretic and dragged off by the crowd (and presumably killed), all amidst protests from Brian.
• Loretta crying after Reg lays into her at the Gladiator game, especially if something similar has happened to you or a loved one.