Shinji slitting his wrists back in 2020 because he thought Asuka hated him.
In chapter 2 Asuka has a nightmare where she relives all her defeats and humiliations during the Angel War up to the moment where she felt the MP-Evas tearing her alive and eating her. Then she wakes up and starts crying.
Gendo dying in Shinji's arms while his son says he forgives him.