Films - Animated
- In Lady and the Tramp, a poster for Uncle Tom's Cabin can be seen on the wall of Tony's restaurant.
- Gangs of New York features a performance of the play.
- In Language Arts, Charles reads excerpts from Uncle Tom's Cabin in Language Arts class.
- In Hitty, Her First Hundred Years, Clarissa's family, who are Quakers and abolitionists, read passages from Uncle Tom's Cabin. Clarissa is so upset by it that her mother puts a stop to the reading so she won't have nightmares.
- In Gone with the Wind, Scarlett O'Hara is annoyed that Northern women regard Uncle Tom's Cabin as "revelation second only to the Bible."
- M*A*S*H: In the 10th season episode "The Tooth Shall Set You Free", one of the plots involves a racist major who systematically arranges for the black soldiers in his unit to get the toughest assignments and therefore suffer the highest casualty rate, and those who aren't killed he arranges to be shipped home, even if their wounds are minor. When Hawkeye discovers this, he says the slaves in Uncle Tom's Cabin got better odds on living.
- Classic Disney Shorts: The 1933 short Mickey's Mellerdrammer has Mickey and his friends putting on a production of Uncle Tom's Cabin, with Mickey as both Uncle Tom and Topsy, Minnie as Little Eva, Clarabelle Cow as Eliza, and Horace Horsecollar as Simon Legree.
- Looney Tunes:
- The 1937 short Uncle Tom's Bungalow is a parody of the story.
- At the beginning of A Coy Decoy, one of the books seen in the bookstore is a copy of Uncle Tom's Cabin with an F.H.A. sign in front of the cabin.
- In Book Revue, Daffy is chased by a wolf through a copy of Uncle Tom's Cabin, leading to a parody of the "Eliza-on-the-ice" scene.
- In Mississippi Hare, one of Bugs Bunny's tricks on Colonel Shuffle involves him promoting "Uncle Tom's Cabinet".
- Popeye: In Cartoons Ain't Human, Popeye's homemade cartoon features Olive avoiding a pair of bloodhounds by going across a river with ice cubes on her feet.
