In Color Me Perfect, Andy accuses Mitch, who is giving Dina an experimental intelligence-boosting gene therapy, of being like Henry Higgins.
In Doctor in Trouble, Indian steward Satterjee tries to improve his English accent by repeating "The rain in Spain stays mainly in the plains" and "In Hertford, Hereford, Hampshire, hurricanes hardly happen".
In the fourteenth Goblin Slayer light novel the husfreya prepares for a future trip to the capital of her husband's nation by reciting the phrase, "The rain, I explained, stays mainly on the plain. " as part of her practice in speaking her husband's native language properly and thus avoid embarrassing him. Priestess, who is both admiring and amused reflects that the husfreya will always be her husband's fair lady.
In There's More Than One Way Home, Anna feels like she's dumber than her husband Alex. She thinks, "Henry Higgins married Eliza Doolittle but after twelve years she still couldn't sing 'The Rain in Spain.'"
In The Monster Garden, Frankie goes to the Playhouse with Hazel and her parents, and comes home singing "Wouldn't It Be Loverly".
The fourth episode of Chockablock has Chockagirl record herself singing "The Rain in Spain". This is one of the few occasions where Chockablock doesn't mess up the recording by missing out words which fit the rhyme around which the episode is themed. Not only that, he and Chockagirl turn the whole thing into a duet, with Chockagirl singing Higgins's part while Chockablock sings Eliza's part.
Since Were You Raised by Wolves? is a podcast where the hosts teach people about manner — often high etiquette — Nick and Leah will reference the film whenever the opportunity arises.
Family Guy: Because Stewie's voice was based on Rex Harrison's, there were at least a couple times he referenced his most famous role.
In "Running Mates", Stewie performs "I've Grown Accustomed to Your Face" when he realizes that he misses having Lois around while she's off campaigning.
The subplot for "One If by Clam, Two If by Sea" has Stewie trying to teach a Cockney-accented toddler how to speak properly, culminating them singing a song to the quote "The life of the wife is ended by the knife".
Foster's Home for Imaginary Friends: In "My So-Called Wife", Mr. Herriman tries to teach Coco how to act proper in order to pose as his wife for a fancy event, while Mac tries to teach Bloo to learn how to be sarcastic (or at least realize he's being sarcastic). When they succeed, they both exclaim "By George, I think you've got it!".
Phineas and Ferb: The episode "My Fair Goalie" is not only named after the musical, but Candace's plot line is a Whole-Plot Reference to it. To drive it home, Ferb's cousin (who isn't named after David Beckham) is named "Eliza".
VeggieTales: In "The Star of Christmas", as our heroes are about to leave for the Christmas pageant on a high-speed rocket car, Milward (Larry) shouts "Get me to the church on time!".
pokes fun at the characters and twists things around for comedy.