The Far Side: A 1991 panel has the caption "How Mr. Ed was made to talk". The cartoon features a horse in front of the camera with a caged lion just offstage, implying that he spoke with the threat of being eaten.
Phoebe and Her Unicorn: One storyline has Phoebe showing Marigold a show with a talking horse, saying that they made the horse "talk" by feeding it peanut butter (as was alleged for Mister Ed). She then tries feeding Marigold peanut butter to see if it makes her talk better, only for it to have the opposite effect.
All in the Family: In "Edith's Conversion", Edith eats a horse meat steak and says she can't help but think about Mister Ed.
Here's Lucy: In "Lucy Competes with Carol Burnett", Lucy compares beauty contests to horse shows, to which Carol retorts "Well, comb out my tail and call me Mister Ed."
Full House: In "Danny in Charge", Danny calls Michelle "Miss Ed" in response to her pretending to be a horse.
In Living Color!: During the word scramble portion of an Old Train skit, the two contestants have no idea of what the incredibly easy puzzle of "Mr. De" is.
Married... with Children: In "Raingirl" as the family watches Kelly bomb as a anchorwoman on the news due to her inability to read, Bud quips that they should spread peanut butter on her gums so someone else can mimic her talking like they did with Mister Ed.
In "Parker Lewis Can't Win", Ms. Musso tells Parker not to try anything sneaky with the video he agreed to make for her, including "lip-syncing my homecoming speech to Mister Ed," to which he replies "Of course, of course."
In "When Jerry Met Shelly", Shelly says that she couldn't sit through Mister Ed if her life depended on it.
In the Dinosaurs episode, "Hurling Day", the show was spoofed as "Mister Ugh", with the title character being a talking caveman owned by a dinosaur named Woodrow. In a promo for the show, Mister Ugh is advised not to open a very important package addressed to his owner.
Sesame Street: In the Elmo's World episode about Horses, when Elmo decides to talk to a horse, the horse introduces him to his herd, which includes a new colt named Wilbur, named after the show's human lead.
In his list of "Top 11 Saddest Nostalgic Moments", Critic imitates a depressed Mister Ed when going over the scene in The Never Ending Story when Artax drowns in the Swamp of Sadness.
In his review of the five-part DuckTales pilot, Critic notes that Scrooge is voiced by Alan Young and wonders if Scrooge made his fortune by betting on Mister Ed at the races and turning him into glue.
In "Rise of the Commercials", when Critic reviews various commercials for Teenage Mutant Ninja Turtles action figures, he says that the animatronics for the Turtles went from Jim Henson puppetry to dead-eyed corpses with fishing line-pulled lips like Mister Ed.
BoJack Horseman: BoJack's ringtone is the first few notes of the theme song.
Ed, Edd n Eddy: There is an episode titled "They Call Him Mr. Ed".
Family Guy: In "Chick Cancer", Joe is zapped unconscious during a movie shoot and Peter suggests they put peanut butter in his mouth so it'll appear that he's taking.
Muppet Babies (1984): In "The Frog Who Knew Too Much", the babies try to guess what Nanny's surprise for them is. Skeeter hopes it's a horse, to which Gonzo hopes it might even be a talking horse. When Skeeter tells Gonzo that horses can't talk, Mister Ed appears and says "You can say that again, Wilbur."
Pinky and the Brain: In "A Meticulous Analysis of History", the Brain sings about Caligula: "The Romans knew he'd lost his head / When he gate a vacant senate seat to Mister Ed".
Rocko's Modern Life'': In "Boob Tubed", when Heffer plays with a video camera at the electronics store, he says, "Wilbur! Wilbur!" in Mister Ed's voice.
In "Treehouse of Horror XII", Homer calls Lisa "Mister Ed" when she is turned into a centaur.
In "Papa's Got a Brand New Badge", Barney says that "they have Ed Bradley vs. Mister Ed on Celebrity Boxing."
In "Milhouse Doesn't Live Here Anymore", a statue of Mister Ed is on display at the exhibit Things That Shouldn't Talk, But Do at the television museum.
