- Doors very like the ones in this cartoon show up in Forbidden Zone. Squeezit is punched when he opens one of them, and two devils come out of another to take him to Danny Elfman's Devil.
- The cartoon itself appears in the "It's A Good Life" segment in Twilight Zone: The Movie.
Music
- The music video for "Ghost of Stephen Foster" by Squirrel Nut Zippers is based on this and several other Betty Boop shorts and includes references such as the spiral staircase scene.
Video Games
- Cuphead: The Conveyor Belt o' Doom in Pip and Dot's boss battle that pulls you toward a spiked wall is a reference to when Bimbo is faced with a similar trap.
- Mickey Mania, of all games, has a spiral staircase segment in The Mad Doctor level that's strikingly reminiscient of the one featured in this short, complete with 3D rotation and rickety floorboards.
- Ollie & Scoops: In "Old Crumplecranks" (which already references many other Fleischer cartoons), the shot where Poopsie and Rudy slide down the "Deadcat Ditch" is framed in the same way Bimbo slides down the corridor into the axe-filled room.
Western Animation
- The scenes where the door gobbles Bimbo, he is terrified to see his shadow's head chopped off and where his heart leaps out from his throat and he swallows it were copied in the climax of the Adventures of Sonic the Hedgehog episode "Robotnikland". Robotnik and Grounder are seen only experiencing the first one while Scratch experiences all three.
- A brief clip from the short is played in the The Grim Adventures of Billy & Mandy special "Wrath of the Spider Queen" as the titular character is digging through Grim's memories.
- The Cuphead Show!: One of the attractions that the Devil transforms while chasing Cuphead and Mugman in the episode CarnEvil features a dangerous slide with spikes, a room full of saws that the duo barely evades, and sharp closing doors closing behind them.
