- Adam and Joanna from Mad Love (1995) watch Bill Nye the Science Guy in one scene.
- In The Eagle Tree, Stig shares some facts about fire ants that he learned from Bill Nye. March replies, "I like Bill Nye. He's a science guy."
- In one episode of Bones, Temperance Brennan got "Sesame Street" Cred by appearing in a skeleton costume on "Bunson Jude, the Science Dude".
- SuperMarioLogan: In Part 5 of the "Bowser Junior's First Grade" story arc, Junior and Joseph decide to draw a picture of the Sun for a school project on the insistence that it's the biggest and hottest planet in the galaxy. When Cody tries unsuccessfully to tell them that the Sun is a star, Joesph tells him "Who died and elected you Bill Nye the Science Guy?"
Webcomic
- Bil Nye the Russian Spy is obviously a parody of the show, starring Bill Nye himself and his assistant Russian Blue cat Nermal (who are both Russian spies).
- In Action League NOW!, the character of Bill the Lab Guy was named for Bill Nye the Science Guy.
- On The Patrick Star Show, "Dr. Smart Science" has Patrick hosting his own science show. The episode opens with a shot of his head appearing on a TV screen and spinning through space as music plays, in a homage to the Bill Nye opening sequence.
- In the Pinky, Elmyra & the Brain episode, "Better Living Through Cheese", Bob Quack the Science Hack appears as a judge at Chuck Norris Grammar School's science fair.
