Demoted to Extra: In the manga, Lady Oyakata was the main antagonist of the Witch's Knoll Arc. Here, she had long-since been dead and Rubi deluded herself into thinking that she was still alive and giving her orders, thus making Rubi the antagonist.
Easily Forgiven: After Rubi nearly kills Tsukune and the girls retaliate at the end of the last episode, Rubi wakes up to find Tsukune was healed and doesn't hold a grudge in the slightest.
Foreshadowing: Whenever Rubi proclaims her Declaration of Protection over Witch Hill, she punctuates it with the phrase "because it's my wish... because her Ladyship demands it." This is a hint that both reasons are the same reason, the orders from Lady Oyakata being her own thoughts and desires being fed through a fractured mind.
Humans Are the Real Monsters: Rubi's hatred of humans comes from the nearby humans trying to destroy Witch Hill with construction.
Moral Myopia: Rubi claims that the only ones that kill are humans, yet she herself uses Man Eating Plants on any human unfortunate enough to wander into Witch Hill and wants Yukari's help in destroying them.
Parting-Words Regret: The last thing Lady Oyakata ever said to Rubi before her death was that she realized that her campaign against the humans made her just as bad as them.
Power Parasite: In her One-Winged Angel form, Rubi digs one of her vines into Moka with the intent of stealing her latent vampire abilities.
Psychoactive Powers: When Rubi returns to the house after Tsukune and his harem offer her a place at Yokai Academy, she is attacked by vines, looking like a case of You Have Failed Me from Lady Oyakata. With the reveal that Lady Oyakata was actually Dead All Along, this means that the vines attacked Rubi were cast by her, attacking her to keep up her delusions.
