- The fact that this version of Dr. Frankenstein wasn't an evil man. Even taking it upon himself to try and protect his creation from Dracula, treating him sort of like a son. Makes his ultimate fate all the sadder though...Dr. Frankenstein: I could never allow him to be used for such evil.Dracula: I could. In fact, my brides are insisting upon it.
- Similarly, his creation holds no animosity toward him. He's distraught when Dracula kills him, and even refers to him as his father when talking to Anna and Helsing.
- The Cardinal is kind of a jerk to Van Helsing, but he does show a great deal of sympathy and gratitude towards the Valerious family and a determination to save them from being condemned to Purgatory.
- The very existence of Holy Order is, in a way, quite heartwarming. Disciples of different religions — who would otherwise wage wars on each other countless times in history — actually putting aside their differences and working together towards a greater goal. A mullah shouting "what in the name of Allah is wrong with you?" towards Carl is both very funny and very heartwarming when you think about it, because it means any of those disciples may express their own beliefs and absolutely nobody (with different beliefs) bats an eyelid.
- A brief one for the villains; after losing Marishka, Dracula decides to press ahead with his plans to awaken his children. Aleera and Verona beg him not to, insisting they can't go through the heartbreak of losing their offspring again if it fails. Dracula, in something of a bad mood, angrily lets loose a demonic roar at them, causing the pair to retreat wailing in fright, at which point Dracula seems to realise he may have gone too far and gently enfolds the pair in his arms in something of an apology.Dracula: Come. Do not fear me. Everybody else fears me. Not my brides.
- Verona holding one of her babies in her hand for a moment in an otherwise horrifying scene, she is just as happy as any mother seeing her child come to her.
- Carl deciding to go against his orders from Rome to destroy Frankenstein's Monster when it is hanging off a cliff, about to fall, recognising the creature has twice saved his life and deserves a chance.Frankenstein's Monster: Help me!Carl: But you're supposed to die!Frankenstein's Monster: I want to live!Carl: Alright! Hang on, I'll swing you loose!
- Frankenstein's creature's look when Anna thanks him, after he saved her from one of the brides. For someone who has only been treated like a monster or a tool in his life, that thanks meant everything to him, even making him more determined to help the heroes against Dracula.
- Frankenstein's monster being willing to sacrifice itself to keep from being used by Dracula.
- Van Helsing refusing to kill Frankenstein's creation.
Van Helsing: My work - my job, is to vanquish Evil. I can sense Evil. This thing... Evil may have created it, left its mark on it, but Evil does not rule it, so I cannot kill it.Anna: I can. *raises pistol*Van Helsing: *puts pistol down* Not while I'm here. - Future Tear Jerker for all it's worth, but when Van Helsing kissed Anna before they went their separate ways.
- Fridge Brilliance that makes this even more heartwarming. Van Helsing has no memories of anyone or his past and Anna likely spent most of her life training to kill vampires, albeit having family members she loved and lost. It's all but stated on Van Helsing's part that he has never fallen in love before, and implied that Anna hasn't either. It's likely a First Kiss for them both.
- Van Helsing is a jerk to Anna at first when she stops him from killing werewolf Velkan, thinking she's just being a bit naive and foolish. But once she states that she's going to try and find the rumoured cure for lycanthropy to save him, and how he's "the only family I have left", he concedes and tries to comfort her over her losses as well.
- Anna displays an interest in the sea throughout the film, stating she has never been to it and hopes that it's beautiful, and it's likely she's never been able to travel out of Transylvania because of the mission. Van Helsing honours this by having her funeral be by the sea.
- After burning Anna's body, Van Helsing looks into the clouds and sees Anna's spirit reuniting with Velkan and her father, with the outlines of other people, presumably her relatives greeting her. Although she's dead, he gets first hand confirmation that she's been reunited with her beloved family, and all the Valerious clan are going to Heaven because he defeated Dracula.
- We also see a single tear on Anna's face, which tells Van Helsing that she loved him as much as he loved her, and it saddens her to be parted from him even with meeting her family again.
- The village woman Carl saves, giving him a big hug of thanks after he saves her from one of the vampire children.
- Velkan and Anna's protectiveness of each other during the werewolf hunt. Which makes his death all the more heartbreaking.
- "Written by Stephen Sommers...
...in memory of my dad."
