It's stated that Kamui Tokonomiya has clashed with Parace L'Sia several times in the past, yet in gameplay she gets curbstompedjust as easily as the others. However, LOVE MAX!!!!! finally addresses this discrepancy, with a special boss version of Kamui that uses the power of her Time Arcana to the fullest. Her playable version is when Kamui is holding back, so she doesn't seriously hurt her friends.
Whatever happened to the nuns that were assisting Mildred in the original Arcana Heart? After Mildred is defeated, she gets sucked into the Arcana world, and her floating castle vanishes. Either the nuns got sucked in along with her, or it's possible they all died.
Pistrix's official designation is 'Series Pistrix No.29'. Which means there were at least 28 previous attempts at creating a homunculus by Parace L'Sia, and it's likely they were all designated failures and disposed of.
Pistrix's profile states that "No. 29 to call the others during battle to give her the advantage" thanks to Parace L'Sia's alchemy and you even get to see multiple Pistrix in her Critical Heart. It's very unlikely that the previous numbers were just disposed of short of any genetic failures.