It's very symbolic of Josie to turn into a tree when you recall what happened to souls in the Inferno who were guilty of suicide.
Kane's sudden Southern accent in his video message comes out of nowhere, considering he spoke with Oscar Isaac's normal voice for the rest of the film. Except, we've already seen his bear tattoo appear on another squad member and it's established that bits of people's physiologies and personalities end up mixed together inside the Shimmer. He got the accent from a fellow soldier.
In the video of Kane exposing a fellow living soldier's intestines, how did he know to do that? He seems to know what he's about to reveal to the camera, even before proceeding...
