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Family

Mother

Determined Homesteader: She is dedicated and determined to help her family surprise and do whatever it takes to keep things together. However, since she is stuck in a Foolish Husband, Responsible Wife dynamic with her homesteader, she is more of a Deadpan Snarker than is usual for this trope.
Faking the Dead: The mother does this while guilt-tripping the daughter; apparently, she did it quite often in the past. She pulls the same stunt if she's the one who "dies" of dysentery. If the daughter is chosen to die, she does this in order to get back at the mother. The mother begrudgingly promises to never do it again.
Mama Bear: Like woah.
Mother: [to the Bandit King] You see that girl right there? I made her. Out of my blood and my flesh, my sweat and my tears. And I love her more than the wakin' world. But I will smother her in her sleep before I see her ruin herself with some thing like you, so you better just run along now cause you'd have better luck sticking your pecker in a cactus.
Only Sane Man: The only sane woman of the family, as traditional for sitcom moms.
Wet Blanket Wife: Always waiting to put the kibosh on the Father's fun schemes like trying to sprout wings and fly to Oregon.

Father

Played by Jeff Blim

Bumbling Dad
Mother: I'm babysitting, but not my kids!
Sometimes I wonder what the hell goes on in his head,
Would he even notice if any one of use wound up dead?
Who am I kidding, lord known he'd kill himself instead.
Can't say I'd tell a difference in bed.
Catchphrase: "That's what this trip's all about."
Determined Homesteader: Parodied. While Father is determined to get to Oregon, he manages to set his own farm on fire, get swindled into buying a defective wagon and ox, and nearly get himself and the family killed multiple times.
Never My Fault: Claims all of his many failings, including the fire that prompted the trip in the first place, were acts of God.
Took a Level in Badass: After Mother leaves him, he finally gets his act together, robbing the General Store Guy at gunpoint for a new wagon.

Daughter

All Girls Want Bad Boys: She is initially charmed by McDoon.
Daughter: Oh my god, I can't believe it! Mr. McDoon was the Bandit King the whole time, what a rogue!
Mother: What is the matter with you girl? Were you just encouraging that wild man's advances?
Daughter: Yes I was, until you came along and interrupted!
Bratty Teenage Daughter: Will happily mouth off about how her Mother doesn't understand her and openly wishes that her family would all disappear so she can run off with the Bandit King. This disappears quickly when it actually happens.

Son

Catchphrase: "I don't know."
Mother: How did a bird end up in your mouth?
[Beat]
Son: [shrugs] I don't know.
Crosscast Role & Dawson Casting: Unsurprisingly for StarKid, Lauren Lopez plays a little boy.
Son: Yeah that's me, I'm 7 and I'm male!
The Ditz: The son has shades of this, but he is only seven years old...
Extreme Omnivore: If he doesn't know what an object is, he will try to eat it. This includes a dead bird, a scorpion, and a gun.

Grandpa

Catchphrase: "I'm trying!"
Cloudcuckoolander: Subverted. All that crazy shit he was saying about how he fought lobsters, who then banished him to the land, stole his wife, and his million dollar bill? Completely true.
Insane Troll Logic: Farmers use pitchforks. The Devil is sometimes depicted with a pitchfork. Ergo, farmers are the Devil.
Rambling Old Man Monologue: Likes to reminisce about how he fought lobsters in the War of 1812 and banished them to the sea. In private, he admits to the son that this was a lie...they actually banished him to the land, and stole his wife to boot.

Bandits

McDoon the Bandit King

Affably Evil: Is quite politely up-front about his intentions toward the Daughter when the Mother interrupts their first meeting.
McDoon: Woah, hello, ma'am! You ain't got nothin' to worry about! I was just making sexual advances on your daughter, is all.
Character Tics: He instinctually salutes with his middle and forefingers every time he says "the Bandit King". This leads to his and Cletus Jones's probable deaths when he does so while trying to hold on to the Daughter while swept up in the Great Columbia River rapids.
Ephebophile
McDoon: I want to make your daughter my bride, alright alright!
Mother: She's a child.
McDoon: Well my child bride, please and thank you.
Even Evil Has Standards: Played for laughs. Sure, he'll kidnap Daughter to Mexico so he can marry her against her will...but he's not gonna try and sleep with her until then.
Obliviously Evil: He seems genuinely confused why Daughter isn't in love with him after all the nice things he did for her, like chloroforming her to sleep every night and tying her up with expensive ropes.
Situational Sexuality: Is in a relationship with his second Cletus Jones, but makes clear that he doesn't consider it real and will happily jump on the first woman he can kidnap as a new bride.

Cletus Jones

Incompatible Orientation: He's genuinely in love with McDoon, who sees their relationship as Situational Sexuality.
I Want My Beloved to Be Happy: Unfortunately for the Daughter. He won't help her escape (despite being in love with McDoon himself), because he's not a homewrecker.

General Store Guy

Bestiality Is Depraved: Heavily implied to have impregnated his ox, which gave birth to the ox with a human hand he gives to the family.
His Name Really Is "Barkeep": Judging by his interactions with his girlfriend, he actually goes by General Store Guy.
Honest John's Dealership: He sells a broken-down wagon missing part of its bottom and practically falling apart, with octagon wheels, for twice the price of a normal wagon, and also sells an eyeless, toothless, deformed ox that seems to be his Half-Human Hybrid son for an additional fifty dollars while claiming it is "free." After laughing about it to his girlfriend much later, he tries to sell the same wagon, which had burned down, to the Father, claiming it is okay to do so because it is half price.