Note: Names in italics indicates that this character only makes a cameo appearance in The Chorus Trilogy.
- Blood Gulch Crew: Freelancer Agent Carolina, Medical Super-Private First Class Frank "Doc" DuFresne/"O'Malley", Lopez Dos.0/"Dos Point Oh"
- Red Team: Colonel Sarge, Captain Dexter Grif, Captain Richard Simmons, Private Franklin Delano Donut, Lopez The Heavy/Lopez La Pesado
- Blue Team: Captain Lavernius Tucker, Captain Michael J. Caboose, Freelancer Agent Washington/"Wash"/David, Private Kaikaina "Sister" Grif, Freelancer Agent Texas/"Tex"/Allison, Freckles
- Project Freelancer: Freelancer Agent New York/"York", Freelancer Agent Wyoming/Reginald/"Reggie", Freelancer Agent Maine/"The Meta", Freelancer Agent Connecticut/C.T./"Connie", Freelancer Agent South Dakota, Freelancer Agent North Dakota, The Director of Project Freelancer/Dr. Leonard Church, Counselor Aiden Price, F.I.L.S.S.
- Freelancer Artificial Intelligences: Omega/"O'Malley", Gamma/"Gary", Delta, Theta, Eta & Iota
- Charon Industries: The Chairman/CEO Malcolm Hargrove, Isaac Gates/"Felix", Samuel Ortez/"Locus", Flame Soldier/"Sharkface", Zachary Miller
- Chorus: General Vanessa Kimball, General Donald Doyle, Lieutenant John-Elizabeth Andersmith, Lieutenant Katie Jensen, Lieutenant Antoine Bitters, Lieutenant Charles Palomo, Dr. Emily Grey, Matthews, Jason Cunningham, Rogers, The Rebel Medic, The Fed Gunner, Privates B.B. Ghanoush and B.H. McCallister
- Others: The Crew of the Tartarus, "Santa"
