Fafa
- Butt-Monkey: Whenever Gorilla decides to strong-arm him.
- Funny Animal
- Cloudcuckoolander: More than Mario, surprisingly.
- Heterosexual Life-Partners: With Mario.
- Red Oni, Blue Oni: Fafa is the Blue Oni, despite being brown.
- Stiff Upper Lip: Never loses his cheery, earnest demeanor, even in the face of the end of the world.
- Straight Man: Even when he's being a Cloudcuckoolander, he tends to be less excitable and off-the-wall than Mario.
- Talking Animal
Mario
- Amazing Technicolor Population: He is tomato red.
- Berserk Button: Does not like ABBA or vertical videos (although the former is explained as a hate of palindromes)
- Brought to You by the Letter "S": His "M" T-shirt
- Celeb Crush: Is "saving himself" for Katy Perry
- Cloudcuckoolander's Minder: Has to be Fafa's more times than you would think.
- Heterosexual Life-Partners: With Fafa.
- No Social Skills: Needed to be told that it was inappropriate to take off your pants and shout at the screen in a movie theater.
- Only Sane Man: When the situation becomes REALLY insane. Especially in The Pee-wee Herman Blog.
- Red Oni, Blue Oni: Naturally, the more excitable and red Mario is Red Oni to Fafa's Blue Oni.
- Take That!: Is often prone to openly telling people and things that they suck
- Too Dumb to Live: Sometimes. Instead of just escaping the Saw parody, he puts on the Lollipop Hat.
- What the Hell Is That Accent?: Speaks in what sounds like an Eastern European accent
Gorilla
- Butt-Monkey: In the Photoshop Tutorial.
- Jerk with a Heart of Gold: Generally a well-meaning guy, but Gorilla has been shown strong-arming people (usually Fafa)
- Recurring Character: The most prominent character after Mario and Fafa.
- The Unintelligible: Only says "Meh". Even when singing.
Johnny T
- Brooklyn Rage: Hails from the Bay Ridge neighborhood, specifically.
- Catchphrase: "Boom, done!"
- "Whaddaya freakin' kidding me?!"
- "In conclusion..." when he's wrapping up a discussion.
- Hand Wave: His answer when Mario asks how a toad can be related to a groundhog?Johnny: My dad was Italian.
- Hidden Depths: Knows some astronomy about the Sun and Moon.
- Smarter Than You Look: A swaggering Guido stereotype. And smart enough to get into Berkeley College.
Thor
A heavily exaggerated version of Thor, complete with booming voice and arrogant personality- Berserk Button: Thou shalt NOT insult Natalie Portman!
- Hidden Depths: Is a decent singer, particularly with his rendition of Marvin Gaye's "Let's Get It On".
- Jerkass
- No Indoor Voice
- Recurring Character
Zombie
A zombie (akin in design to those from Plants vs. Zombies) who makes appearances- Butt-Monkey: In "Zombie University" where he's the practice dummy used by Mario and Fafa to demonstrate zombie slaying techniques.
- Everything's Deader with Zombies
- Hidden Depths: Like Thor, he is a decent singer when it comes to "What a Wonderful World".
- Omniglot: At the very least, he knows how to say "Brains" in virtually every language on Earth-even sign language.
- The Unintelligible: When acting as a zombie, otherwise he is capable of speech.
Jack Woods
A nature specialist who is quite knowledgeable about rabbits...and may be insane.- Ax-Crazy: Mario believes it, and there is something off about the guy.
- The Nicknamer: Refers to everyone as "Johnny"
Blake Worthington and Bungo
A pair of news anchors. Blake is a chimpanzee and Bungo is an Orangutan.- Aerith and Bob
- Erudite Orangutan: Averted. While not stupid (especially compared to Mario), Bungo is much more of a casual everyape in comparison to his more formal co-anchor.
- Intelligent Primate: They are two news reporters who happen to be apes.
- Take That!: Their first appearance they exemplify everything bad about American News, they seemed to have dropped this in their second appearance.
Jerkface
A troll that hates Glove and Boots, used to represent trolls and other rude or otherwise malicious people out there on the internet.- Catchphrase: "I think X Stinks"
- Establishing Character Moment: His introduction in "The History of Trolls".Jerkface: Caps lock. "KATE UPTON IS A MANDUDE!" Bold! Italic! SEND!
- Evil Laugh: Very prone to this, along with phlegmy snorting.
- Troll: Horns and all.
Uncle Joe
- Cool Uncle: The fanbase certainly thinks so.
- Groin Attack: Nearly neutered his friend when he was 9 with a bow and arrow. His friends mother was more angry about the hole he put in his pants.
- Irony: One of his stories is titled "Joe vs Squirrels" and is about him dealing with a bunch of them. See the image above for why this is irony.
Hanzi
A fast-talking con man.- Cloudcuckoolander
- Con Man
- Motor Mouth
- New Job as the Plot Demands: Has been seen as a salesman, a YouTuber, and a game show host.
- What the Hell Is That Accent?: Seems to have a vaguely French or Italian accent.
