- Tiffany in The Secret Life of the Backyard Kids. In an interesting twist, she's also a Dark Action Girl hell-bent on world domination.
- Lisa Simpson of all people is cast as an Alpha Bitch in the Phineas and Ferb crossover fic The Flynns Move To Springfield. Vain? Check. Spoiled? Check. Complete Jerkass? Check. Treats others like dirt? Check. Believes that she should get everything she wants? Check.
- Rachel The Vampire Slayer: Melissa is much more of a mean girl in this than in the Animorphs books, as she's filling Cordelia's role.
- Rick and Morty New Drama Adventures: In Chapter 7, “Summertime”, it is shown that Heather is the alpha bitch of the school. She's pretty, captain of the cheer squad, and looks down at all the other girls, including her own friends!
- Alexis Rhodes and Seto Kaiba are this in Ultimate Re-Imaginings, the former also being a Rich Bitch.
- In the DCAMU & Sonic crossover fic Flashpoint 2: Advent Solaris, there is a character brought in that serves as this towards Princess (later Duchess) Elise of Soleanna, her ladies-in-waiting and even Espio. Later in the fic, we are introduced to Komand'r & Talia al Ghul, who both try to basically compete with another for the title of Alpha Bitch amongst the villains.
- Resurrected Memories: Paulina is this to a T.
- When Ember was alive, she had dealt with an even worse one named Alexandria Green, who was not only pretty and wealthy, but severally bullied Ember too.
- Athens from "Athens And Sparta Adventures" (a Fan Webcomic about the personifications of the two two titular city-states, which is inspired but not constrained by Axis Powers Hetalia) is a Rare Male Example (though the setting isn't in High School). Though no one can deny that he's charismatic, intellectual and a centre of arts and lights, he's also arrogant, manipulative, Narcissist, treats everyone (even his closest friends and allies) like dirt and wants everything done in his own way. This behaviour, as you would expect, doesn't make him likeable.Athens (to his allies): And don't try to revolt again! Your tribute money is going straight to the army that'll put you back down! Didn't you get the memo? "If anyone in the cities strikes silver coins and does not use the currency, weights and measures of the Athenians, but foreign currency, weights and measures… exact penal retribution". A court issue? No no no no, if we want to keep criminals in check, we should use the same court. Mine. You know that food you're eating? You owe it to the grain-mother, the Goddess Demeter… Did I mention she was Athenian too? You basically owe us for that food.
- In Glee Reprise, all three leaders of Vocal Adrenaline are pretty smug and hold considerable sway over the student body.
- My Immortal
- This fanfic is, in a nutshell, what you get when a story has an Alpha Bitch as its protagonist yet no attempt is made to portray what she does as bad.
- The story itself has a Gryffindor prep named Britney who is treated as an Alpha Bitch yet doesn't actually do anything bitchy. That's reserved for our hero, Ebony.
- Cho Chang in A Very Potter Musical has a lot of Alpha Bitchy traits who picks on less popular girls. It's taken up to eleven in the sequel.Cho: Come on girls, let's go show Moaning Myrtle our ball gowns and make fun of her because she can't go.
Film/Heathers
- They're All Girlfriends AU has Heather Chandler start out as this, as an extension of her film counterpart. However, she later turns into more of a lovable alpha bitch instead, especially after getting together with Veronica.
- Ruby Pair has Jessica, who is obsessed with popularity and is condescending to anyone who she feels is unpopular. Zita swears she's not that bad when you get to know her, but Tenn isn't buying it.
Law & Order: Special Victims Unit
- Lucy Horowitz is this in the fanfic Girls' School. She suffers a Villainous Breakdown at the end.
- In Too Much Too Much we have Serafina Sforza, a snobby and stuck-up young girl who looks down on Giulia for her tomboyish personality and her unkempt looks and doesn't invite her to her party because of it.
My Little Pony: Friendship Is Magic
- Twilight Sparkle in Friendship is Witchcraft.
- If Rainbow Dash isn't made into a Jerk Jock or Dumb Jock using Ron the Death Eater, she's made into this in stories like Pattycakes (where she was a Flight Camp bully to Fluttershy and abused Scootaloo in the present), Ultra Fast Pony, where she even has a stereotypical Alpha Bitch style voice, as well as Friendship is Witchcraft where she is so self absorbed she speaks Pokémon. (That is, says he name over and over)
- Advice and Trust: Shinji's classmate Chihiro Tanaka. She is not so bad deep down, but she is popular, bratty, and obsessed with Shinji. She insistently tries to hang out with him, in spite of his reiterated negatives, and she does not hesitate on trying to put Asuka and Rei down only because Shinji is extremely close to them ("trying" is the keyword here. Unfortunately for her, bullying Rei is a ''bad'' idea. Bullying Asuka is downright suicidal).
- Once More With Feeling (Crazy-88): Chihiro Tanaka acts like this, being the most beautiful girl in the school, having plenty followers and slandering Shinji after being turned down by him.
- Ships Ahoy!: Just as in canon, Olga fits this trope like a glove. She belittles Oprah for failing her Odd Squad entrance exam (not knowing it was intentional on Oprah's end), abuses her partner O'Donahue, and is an absolutely insane Yandere. There's a good reason why she's referred to as "the epitome of stuck-up, high-and-mighty Odd Squad agents", and it's why Oprah thinks that every Odd Squad agent in existence is an Alpha Bitch as well.
- Mommy Long Legs is this in Poppy Playtime: Break Time. She is also the mix of a Barbaric Bully and Girly Bruiser, not to mention almost ripping Huggy Wuggy apart due to worrying about loss of popularity. She often teases with her Innocent Beta Bastard Bunzo Bunny and tries to earn Poppy Playtime's favor as she envies Huggy Wuggy and slut-shames Kissy Missy.Mommy Long Legs: Grr! UNBELIEVABLE! ONLY I CAN BE THE GREATEST ELASTIC TOY AROUND IN THIS COMPANY! NO ONE WILL EVER UPSTAGE ME!!!!!!
- Sakura of Pretty Cure Full Color is a reformed Alpha Bitch. She gains her powers only when she fully accepts and befriends the people she used to make fun of.
- Omemi Emiru from Futari Wa Pretty Cure Blue Moon is a variation on the concept. Rather than a beautiful blonde who stands out from the crowd (that would be Seira, a member of her Girl Posse whom she strictly keeps in line), Emiru has short green hair and Scary Shiny Glasses, and prefers to watch (and gossip) from the shadows. This understated approach has allowed her to obtain blackmail on nearly every girl in school, and by the time the story starts, they know it and bend to whatever she says. Considering one of the main themes of the story, eventually, this isn't enough for her.
- Every single society girl ever seen on-screen in Four Deadly Secrets. Weiss rules them all.
- In Velma Dinkley's Beginning, we have Jessie, who's the twin sister of Red Herring. She and the other popular girls beat up Velma, because a nerd like her talked to their friend Daphne and crush Fred. She even threatens to have her brother beat up Shaggy if she doesn't stay away from them.Jessie: Look Dorkley, I don't know how you talk to Freddie without him ignoring you but that stops now. If you ever go near Freddie again, you're dead.
- Forum of Thrones:
- This is what Argella Durrandon appears to be like in her initial appearance. She is incredibly vain, haughty and catty when provoked even the slightest bit. Her first real scene even has her brutally mocking a group of diplomats from Dragonstone after her father severed the hands of their leader. However, later chapters firmly subvert this trope, by giving her the chance to show a much more positive side of hers.
- Sherryl plays this trope straight. Next to her, many other characters appear almost saintly in their behaviour. Aside from being one of the driving forces behind a majority of the schemes going on in Raylansfair, she is incredibly smug, arrogant and downright condescending in her interaction with most characters. For her goals, she is even willing to betray old friends and to callously get the citizens of Raylansfair killed.
- Princess Ayeka gets turned into one of these in some fanfics, depending on the author's preferences.
- Total Drama: Unfinished Business: Heather and Amy, as per canon.
- Unbreakable Red Silken Thread
- At the start of the story, this was basically Heather in a nutshell, though thanks to her relationship with Cody she has mellowed out a great deal over the years. However she still has her moments, and won't hesitate to go full "Bitch Mode", as Sugar learned first hand.
- Heather's mother, who is so bad that she makes her daughter seem like a saint in comparison.
- Although not shown in person, Dakota Milton seems to be this too once she took Heather's spot as queen bee at their high school based on what Heather said about her.
- The first entry of the Tales of the Undiscovered Swords gives us a male version with OC sword Himetsuru Ichimonji who acts like a whiny and self-centered self-proclaimed princess for most of the story, to the point where all of the other swords but one are tired of him. That is, until he starts showing Hidden Depths and kicking some ass at the end.
- EVERY FREAKIN' BODY in Tokyo Mew Mew No Hope Left.