- Word of Gay: Fukami Makoto, the writer for the movie, mentions in the movie's commentary that he wrote Nadia's background and that she is a lesbian with a partner living in San Francisco.
- Misc. Trivia:
- Interestingly, this is the first Resident Evil animated movie set in the main video game continuity to feature a real-world city as one of the locations for an outbreak (New York City).
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