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** If ComicBook/PoisonIvy betraying her longtime friend Harley and joining the Society wasn't bad enough, but when she uses her pheromones to take control of Harley and nearly ''poisons'' her with said pheromones is when she really crosses it.

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** If ComicBook/PoisonIvy betraying her longtime friend Harley and joining the Society wasn't bad enough, but when the moment she uses her pheromones to take control of Harley and nearly ''poisons'' her with said pheromones is when she really crosses it.
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* If ComicBook/ThePenguin didn’t cross it by blowing up a bridge and leaving his own men to die (and laughing about it), he definitely crossed it when he [[CopKiller shot a cop]] in front of Batman just to taunt him. Listening to his audio tapes reveals that Penguin probably crossed it long before the game started by torturing and murdering his own men in horrific ways and stuffing them up in his museum.
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[[caption-width-right:350:[[WouldHurtAChild Now you have done it, Superman.]]]]

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[[caption-width-right:350:[[WouldHurtAChild Now you have you've done it, Superman.]]]]
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* Garfield "Firefly" Lynns crosses it when he [[HostageSituation holds a dozen people hostage on the Gotham Pioneer Bridge]] with the intention of luring both Batman and most of the GCPD there, so he can blow it up to kill everyone once they're all gathered. Even when Batman informs the masked arsonist that the bounty on his head has been called off, Firefly acknowledges it... but [[ForTheEvulz continues his attempted mass-murder regardless, purely out of sadistic joy]].

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* Garfield "Firefly" Lynns crosses it when he [[HostageSituation holds a dozen people hostage on the Gotham Pioneer Bridge]] with the intention of luring both Batman and most of the GCPD there, so he can blow it up to and kill everyone once they're all gathered. Even when Batman informs the masked arsonist that the bounty on his head has been called off, Firefly acknowledges it... but [[ForTheEvulz continues his attempted mass-murder regardless, purely out of sadistic joy]].

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* We already knew how much of a deranged sadist Professor Pyg was, but his actions in the Gotham Story ''Dollotron'' firmly establish just how monstrous he can be. As part of a tour for his Circus Of Strange, Valentin [[WouldHurtAChild abducts a young boy and agonizingly turns him into a Dollotron through a series of brutal surgeries]], proudly [[KickTheDog showing off his handiwork to the boy's father]]. The traumatized man offers Pyg a significant sum of money to just have his son back, at which point Pyg shakes his head and [[PreMortemOneLiner utters the following ominous line]], before siccing a large group of other Dollotrons -- several of whom are noted to have previously been children and their parents -- on the man with the intention of [[TheAssimilator inflicting the same horrors upon him]].

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* We already knew how much of a deranged sadist Professor Pyg was, but his actions in the Gotham Story ''Dollotron'' firmly establish just how monstrous he can be. As part of a tour for his Circus Of Strange, Valentin [[WouldHurtAChild abducts a young boy boy]] and [[FateWorseThanDeath agonizingly turns him into a mindless Dollotron through a series of brutal surgeries]], proudly [[KickTheDog showing off his handiwork to the boy's father]]. The traumatized man offers Pyg a significant sum of money to just have his son back, at which point Pyg shakes his head and [[PreMortemOneLiner utters the following ominous line]], before siccing a large group of other Dollotrons -- several of whom are noted to have previously been children and their parents -- on the man with the intention of [[TheAssimilator inflicting the same horrors upon him]].

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* We already knew how much of a deranged sadist Professor Pyg was, but his actions in the Gotham Story ''Dollotron'' firmly establish just how monstrous he can be. As part of a tour for his Circus Of Strange, Valentin [[WouldHurtAChild abducts a young boy and agonizingly turns him into a Dollotron through a series of brutal surgeries]], proudly [[AndShowItToYou showing off his handiwork to the boy's father]]. The traumatized man offers Pyg a significant sum of money to have his son back, at which point Pyg shakes his head and [[PreMortemOneLiner utters the following ominous line]], before siccing a large group of other Dollotrons -- several of whom are noted to have previously been children and their parents -- on the man with the intention of [[TheAssimilator inflicting the same horrors upon him]].

to:

* We already knew how much of a deranged sadist Professor Pyg was, but his actions in the Gotham Story ''Dollotron'' firmly establish just how monstrous he can be. As part of a tour for his Circus Of Strange, Valentin [[WouldHurtAChild abducts a young boy and agonizingly turns him into a Dollotron through a series of brutal surgeries]], proudly [[AndShowItToYou [[KickTheDog showing off his handiwork to the boy's father]]. The traumatized man offers Pyg a significant sum of money to just have his son back, at which point Pyg shakes his head and [[PreMortemOneLiner utters the following ominous line]], before siccing a large group of other Dollotrons -- several of whom are noted to have previously been children and their parents -- on the man with the intention of [[TheAssimilator inflicting the same horrors upon him]].

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Added entries for Firefly and Professor Pyg.


* Even though he's an InsufferableGenius, ComicBook/TheRiddler's always been held up as one of Batman's lighter and less-sadistic villains. While he had put various security guards in elaborate death traps, at least there was always the chance of Batman being able to save them. That's why it's so genuinely shocking to see him put Cash and the other remaining guards in a death trap that will inevitably kill them, allowing him to watch them slowly walk and walk until they're too exhausted to continue and they meet their end. All while he taunts them for being too ignorant to escape on their own. If you look around the control room, you'll find a "scoreboard" that shows a few of them had already met their end before Batman came to the rescue.

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* Even though he's an InsufferableGenius, ComicBook/TheRiddler's always been held up as one of Batman's lighter and less-sadistic villains. While he had put various security guards in elaborate death traps, at least there was always the chance of Batman being able to save them. That's why it's so genuinely shocking to see him put Cash and the other remaining guards in a death trap that will inevitably kill them, allowing him to watch them slowly walk and walk until they're too exhausted to continue and they meet their end. All get blown up by motion-sensitive bombs; all while he taunts them for being too ignorant to escape on their own. If you look around the control room, you'll find a "scoreboard" that shows a few of them had already met their end before Batman came to the rescue.



* ComicBook/TheScarecrow already proves he isn't playing around when he threatens to douse the city in fear toxin, but the moment he [[KickTheDog forces Gordon to lure Batman to him, then throws Barbara off the construction building when Gordon seemingly kills Batman]] is the moment he truly crosses the line.
* An in-universe example; the act that made Jason Todd decide that the Joker was utterly irredeemable and had to die was when he blew up a school filled with kindergarteners and crudely stitched their body parts together, with the children's mothers fighting over their "patchwork corpses."
* ComicBook/HarleyQuinn was never the nicest girl in town, but her actions in ''A Matter of Family'' were horrific, even if she only was an accomplice. Just to recap: she helped ComicBook/TheJoker con the desperate rich father of a [[LittlestCancerPatient little girl with terminal cancer]] into building an amusement park for her just so the Joker and Harley could use it as their secret hideout once the father and daughter died; helped turn said little girl into a test subject for the TITAN formula with the promise that it would cure her (which also doubles as a MoralEventHorizon in retrospective for [[VideoGame/BatmanArkhamAsylum Dr. Young from the first game]]), but was what caused her painful death; [[DrivenToSuicide drove the grieving father into suicide]] by rubbing salt into the would AND gave him a drug that would supposedly kill him quickly and painlessly ''but was actually Joker serum'' that gave him a painful death between laughing fits.

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* ComicBook/TheScarecrow already proves he isn't playing around when he threatens to douse the city in fear toxin, toxin on several occasions, but the moment he [[KickTheDog forces Gordon to lure Batman to him, then throws Barbara off the construction building when Gordon seemingly kills Batman]] is the moment he truly crosses the line.
* An in-universe example; the act that made Jason Todd decide that the Joker ComicBook/TheJoker was utterly irredeemable and had to die was when he blew up a school filled with kindergarteners and crudely stitched their body parts together, with the children's mothers fighting over their "patchwork corpses."
corpses". For one last even ''worse'' touch, Joker sings "Humpty Dumpty" to said mothers over the intercom to spitefully taunt them... and it's revealed that he was counting on Jason coming after him, setting a trap at Arkham Asylum to capture him, before horrifically torturing him over several months and ultimately leading to Jason becoming the Arkham Knight.
* ComicBook/HarleyQuinn was never the nicest girl in town, but her actions in ''A Matter of Family'' were horrific, even if she only was an accomplice. Just to recap: she helped ComicBook/TheJoker con the desperate rich father of a [[LittlestCancerPatient little girl with terminal cancer]] into building an amusement park for her just so the Joker and Harley could use it as their secret hideout once the father and daughter died; helped turn said little girl into a test subject for the TITAN formula with the promise that it would cure her (which also doubles as a MoralEventHorizon in retrospective for [[VideoGame/BatmanArkhamAsylum Dr. Young from the first game]]), but was what caused her painful death; [[DrivenToSuicide drove the grieving father into suicide]] by rubbing salt into the would AND gave him a drug that would supposedly kill him quickly and painlessly ''but was actually Joker serum'' Venom'' that [[DieLaughing gave him a horribly painful death between laughing fits.
fits]].
* Garfield "Firefly" Lynns crosses it when he [[HostageSituation holds a dozen people hostage on the Gotham Pioneer Bridge]] with the intention of luring both Batman and most of the GCPD there, so he can blow it up to kill everyone once they're all gathered. Even when Batman informs the masked arsonist that the bounty on his head has been called off, Firefly acknowledges it... but [[ForTheEvulz continues his attempted mass-murder regardless, purely out of sadistic joy]].
* We already knew how much of a deranged sadist Professor Pyg was, but his actions in the Gotham Story ''Dollotron'' firmly establish just how monstrous he can be. As part of a tour for his Circus Of Strange, Valentin [[WouldHurtAChild abducts a young boy and agonizingly turns him into a Dollotron through a series of brutal surgeries]], proudly [[AndShowItToYou showing off his handiwork to the boy's father]]. The traumatized man offers Pyg a significant sum of money to have his son back, at which point Pyg shakes his head and [[PreMortemOneLiner utters the following ominous line]], before siccing a large group of other Dollotrons -- several of whom are noted to have previously been children and their parents -- on the man with the intention of [[TheAssimilator inflicting the same horrors upon him]].
-->'''Pyg:''' Not your son now. ''My'' Dol-lo-tron.
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** [[Characters/BatmanTheJoker The Joker]] crosses it big-time with the act that gave birth to the ''Injustice''-verse — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman think she was Doomsday. To make things ''worse'', this triggers a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to drive Superman to murder him, something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.

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** [[Characters/BatmanTheJoker The Joker]] crosses it big-time with the act that gave birth to the ''Injustice''-verse — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman think she was Doomsday. To make things ''worse'', this triggers a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to [[StrikeMeDownWithAllOfYourHatred drive Superman to murder him, him]], something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.
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*** Regime Superman killing ComicBook/GreenArrow is the moment he truly crosses the MoralEventHorizon, as it is the first time he kills another hero.
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* An in-universe example; the act that made Jason Todd decide that the Joker was utterly irredeemable and had to die was when he blew up a school filled with kindergarteners and crudely stitched their body parts together, with the children's mothers fighting over their "patchwork corpses."
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[[caption-width-right:350:[[WouldHurtAChild Now you have done it Superman]]]]

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[[caption-width-right:350:[[WouldHurtAChild Now you have done it Superman]]]]it, Superman.]]]]



* ComicBook/RasAlGhul is definitely shown to be insane, when he threatens to kill [[ComicBook/TaliaAlGhul Talia]], his own daughter, to make Batman kill him and take his place.

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* ComicBook/RasAlGhul is definitely shown to be insane, insane when he threatens to kill [[ComicBook/TaliaAlGhul Talia]], his own daughter, to make Batman kill him and take his place.



** [[Characters/InjusticeSuperman Regime Superman]] crosses this line when he burns a hole right through the skull of Regime Shazam for daring to turn around on his word of a new world order. Granted, Supes had done a lot more prior as the high councilor of the [[Characters/InjusticeOneEarthRegime Regime]], but when he does that, on top of giving orders to destroy Gotham City and Metropolis (the very cities he had gone KnightTemplar in order to protect from another tragedy like the one that took Metropolis and launched his StartOfDarkness) to punish the people that are siding with the Insurgency, it provokes Regime Flash to pull a HeelFaceTurn. It is this action that establishes Regime Superman as an irredeemable tyrant that has truly crossed the line.
** [[Characters/BatmanTheJoker The Joker]] crosses it big-time with the act that gave birth to the Injustice Universe — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman hallucinate that she was Doomsday. To make things ''worse'', this would activate a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to drive Superman to murder him, something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.

to:

** [[Characters/InjusticeSuperman Regime Superman]] crosses this line when he burns a hole right through the skull of Regime Shazam for daring to turn around on his word of a new world order. Granted, Supes had done a lot more prior as the high councilor High Councilor of the [[Characters/InjusticeOneEarthRegime Regime]], but when he does that, on top of giving orders to destroy Gotham City and Metropolis (the very cities he had gone KnightTemplar in order to protect from another tragedy like the one that took Metropolis and launched his StartOfDarkness) to punish the people that are siding with the Insurgency, it provokes Regime Flash to pull a HeelFaceTurn. It is this action that establishes Regime Superman as an irredeemable tyrant that has truly crossed the line.
** [[Characters/BatmanTheJoker The Joker]] crosses it big-time with the act that gave birth to the Injustice Universe ''Injustice''-verse — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman hallucinate that think she was Doomsday. To make things ''worse'', this would activate triggers a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to drive Superman to murder him, something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.
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** Sadly for Batman/Bruce, [[AdaptionalVillainy Thomas Wayne]] crossed it by deliberately subjecting Esther Cobblepot to a FateWorseThanDeath just to get her family's estate.

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** Sadly for Batman/Bruce, [[AdaptionalVillainy [[AdaptationalVillainy Thomas Wayne]] crossed it by deliberately subjecting Esther Cobblepot to a FateWorseThanDeath just to get her family's estate.
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** Sadly for Batman/Bruce, Thomas Wayne crossed it by deliberately subjectingEsther Cobblepot to a FateWorseThanDeath just to get her family's estate.

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** Sadly for Batman/Bruce, [[AdaptionalVillainy Thomas Wayne Wayne]] crossed it by deliberately subjectingEsther subjecting Esther Cobblepot to a FateWorseThanDeath just to get her family's estate.
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** [[Characters/InjusticeJoker The Joker]] crosses it big-time with the act that gave birth to the Injustice Universe — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman hallucinate that she was Doomsday. To make things ''worse'', this would activate a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to drive Superman to murder him, something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.

to:

** [[Characters/InjusticeJoker [[Characters/BatmanTheJoker The Joker]] crosses it big-time with the act that gave birth to the Injustice Universe — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman hallucinate that she was Doomsday. To make things ''worse'', this would activate a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to drive Superman to murder him, something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.
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** If Poison Ivy betraying her longtime friend Harley and joining the Society wasn't bad enough, but when she uses her pheromones to take control of Harley and nearly ''poisons'' her with said pheromones is when she really crosses it.

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** If Poison Ivy ComicBook/PoisonIvy betraying her longtime friend Harley and joining the Society wasn't bad enough, but when she uses her pheromones to take control of Harley and nearly ''poisons'' her with said pheromones is when she really crosses it.
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* Ra's al Ghul is definitely shown to be insane, when he threatens to kill Talia, his own daughter, to make Batman kill him and take his place.
* Even though he's an InsufferableGenius, the Riddler's always been held up as one of Batman's lighter and less-sadistic villains. While he had put various security guards in elaborate death traps, at least there was always the chance of Batman being able to save them. That's why it's so genuinely shocking to see him put Cash and the other remaining guards in a death trap that will inevitably kill them, allowing him to watch them slowly walk and walk until they're too exhausted to continue and they meet their end. All while he taunts them for being too ignorant to escape on their own. If you look around the control room, you'll find a "scoreboard" that shows a few of them had already met their end before Batman came to the rescue.

to:

* Ra's al Ghul ComicBook/RasAlGhul is definitely shown to be insane, when he threatens to kill Talia, [[ComicBook/TaliaAlGhul Talia]], his own daughter, to make Batman kill him and take his place.
* Even though he's an InsufferableGenius, the Riddler's ComicBook/TheRiddler's always been held up as one of Batman's lighter and less-sadistic villains. While he had put various security guards in elaborate death traps, at least there was always the chance of Batman being able to save them. That's why it's so genuinely shocking to see him put Cash and the other remaining guards in a death trap that will inevitably kill them, allowing him to watch them slowly walk and walk until they're too exhausted to continue and they meet their end. All while he taunts them for being too ignorant to escape on their own. If you look around the control room, you'll find a "scoreboard" that shows a few of them had already met their end before Batman came to the rescue.



* Scarecrow already proves he isn't playing around when he threatens to douse the city in fear toxin, but the moment he [[KickTheDog forces Gordon to lure Batman to him, then throws Barbara off the construction building when Gordon seemingly kills Batman]] is the moment he truly crosses the line.

to:

* Scarecrow ComicBook/TheScarecrow already proves he isn't playing around when he threatens to douse the city in fear toxin, but the moment he [[KickTheDog forces Gordon to lure Batman to him, then throws Barbara off the construction building when Gordon seemingly kills Batman]] is the moment he truly crosses the line.



* ''VideoGame/LegoBatman'': Lex Luthor easily crosses it by trying to murder everybody in Wayne Tower, because Wayne snubbed him at the Man of the Year Awards.

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* ''VideoGame/LegoBatman'': Lex Luthor ComicBook/LexLuthor easily crosses it by trying to murder everybody in Wayne Tower, because Wayne snubbed him at the Man of the Year Awards.



** Penguin crosses it when shooting the innocent debate moderator. Even Bruce admits this event cemented that [[ThatManIsDead his best friend is gone]]. If that wasn't enough he tries to kill Harvey by smashing his face with a stage light as opposed to the quick death he gave Mayor Hill who actually deserved it.

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** Penguin ComicBook/ThePenguin crosses it when shooting the innocent debate moderator. Even Bruce admits this event cemented that [[ThatManIsDead his best friend is gone]]. If that wasn't enough he tries to kill Harvey by smashing his face with a stage light as opposed to the quick death he gave Mayor Hill who actually deserved it.
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[[quoteright:350:[[VideoGame/InjusticeGodsAmongUs https://static.tvtropes.org/pmwiki/pub/images/mcvgsfa.jpeg]]]]
[[caption-width-right:350:[[WouldHurtAChild Now you have done it Superman]]]]
VideoGame/DCVideoGames have many instances of [[MoralEventHorizon villains crossing the line into being irredeemable]].

![[Administrivia/SpoilersOff All spoilers are unmarked]]. Administrivia/YouHaveBeenWarned!
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[[AC:''Franchise/BatmanArkhamSeries'']]
* For those who have played it, all that needs to be said is "Protocol 10." For those who haven't, Hugo Strange's plan to eliminate crime in Gotham City is a mass execution of every living person in Arkham City. For the record, Arkham City contains a number of "political prisoners" whose only crime was knowing too much about his plans. When Batman makes him look at the results, his only response is "It's glorious, isn't it?"
* Ra's al Ghul is definitely shown to be insane, when he threatens to kill Talia, his own daughter, to make Batman kill him and take his place.
* Even though he's an InsufferableGenius, the Riddler's always been held up as one of Batman's lighter and less-sadistic villains. While he had put various security guards in elaborate death traps, at least there was always the chance of Batman being able to save them. That's why it's so genuinely shocking to see him put Cash and the other remaining guards in a death trap that will inevitably kill them, allowing him to watch them slowly walk and walk until they're too exhausted to continue and they meet their end. All while he taunts them for being too ignorant to escape on their own. If you look around the control room, you'll find a "scoreboard" that shows a few of them had already met their end before Batman came to the rescue.
* Hush crossed this by murdering six innocent people, cutting off their faces while they were still alive, and making his face a perfect copy of Bruce Wayne's, all to ruin his life over a petty grudge.
* Scarecrow already proves he isn't playing around when he threatens to douse the city in fear toxin, but the moment he [[KickTheDog forces Gordon to lure Batman to him, then throws Barbara off the construction building when Gordon seemingly kills Batman]] is the moment he truly crosses the line.
* ComicBook/HarleyQuinn was never the nicest girl in town, but her actions in ''A Matter of Family'' were horrific, even if she only was an accomplice. Just to recap: she helped ComicBook/TheJoker con the desperate rich father of a [[LittlestCancerPatient little girl with terminal cancer]] into building an amusement park for her just so the Joker and Harley could use it as their secret hideout once the father and daughter died; helped turn said little girl into a test subject for the TITAN formula with the promise that it would cure her (which also doubles as a MoralEventHorizon in retrospective for [[VideoGame/BatmanArkhamAsylum Dr. Young from the first game]]), but was what caused her painful death; [[DrivenToSuicide drove the grieving father into suicide]] by rubbing salt into the would AND gave him a drug that would supposedly kill him quickly and painlessly ''but was actually Joker serum'' that gave him a painful death between laughing fits.

[[AC:''Franchise/{{Injustice}}'' franchise]]
* ''VideoGame/InjusticeGodsAmongUs''
** [[Characters/InjusticeSuperman Regime Superman]] crosses this line when he burns a hole right through the skull of Regime Shazam for daring to turn around on his word of a new world order. Granted, Supes had done a lot more prior as the high councilor of the [[Characters/InjusticeOneEarthRegime Regime]], but when he does that, on top of giving orders to destroy Gotham City and Metropolis (the very cities he had gone KnightTemplar in order to protect from another tragedy like the one that took Metropolis and launched his StartOfDarkness) to punish the people that are siding with the Insurgency, it provokes Regime Flash to pull a HeelFaceTurn. It is this action that establishes Regime Superman as an irredeemable tyrant that has truly crossed the line.
** [[Characters/InjusticeJoker The Joker]] crosses it big-time with the act that gave birth to the Injustice Universe — namely, tricking Superman into killing his wife Lois and their unborn child by taking them up to space without a suit because he was under the effects of a Kryptonite-induced fear toxin that made Superman hallucinate that she was Doomsday. To make things ''worse'', this would activate a DeadMansSwitch that Joker had placed in Lois, which would set off a nuke that Joker had planted in Metropolis, devastating the city and killing millions. And ''then'' he goes on to drive Superman to murder him, something that he was never able to do with his archenemy Batman, in what would prove to be the catalyst to this Superman's StartOfDarkness, sending him down the road to tyranny and evil in the name of preventing another such tragedy from ever happening again.
* ''VideoGame/Injustice2'':
** If Poison Ivy betraying her longtime friend Harley and joining the Society wasn't bad enough, but when she uses her pheromones to take control of Harley and nearly ''poisons'' her with said pheromones is when she really crosses it.
** Gorilla Grodd does it by allying with Brainiac and selling out Earth, not caring even for Gorilla City possibly getting destroyed. [[EvenEvilHasStandards Even the villain group he formed disbanded because of this reason.]]

[[AC:Other Games]]
* ''VideoGame/LegoBatman'': Lex Luthor easily crosses it by trying to murder everybody in Wayne Tower, because Wayne snubbed him at the Man of the Year Awards.
* ''VideoGame/BatmanTheTelltaleSeries'':
** Penguin crosses it when shooting the innocent debate moderator. Even Bruce admits this event cemented that [[ThatManIsDead his best friend is gone]]. If that wasn't enough he tries to kill Harvey by smashing his face with a stage light as opposed to the quick death he gave Mayor Hill who actually deserved it.
** Sadly for Batman/Bruce, Thomas Wayne crossed it by deliberately subjectingEsther Cobblepot to a FateWorseThanDeath just to get her family's estate.
** Vicki Vale, of all people, crosses this by forming the Children of Arkham, getting heaven knows how many people killed, and later drugs Bruce to get him out of the way.
** Riddler already crosses quite a few lines in his introduction -- mutilating Mori with intent to execute him, slitting a casino security guard's throat for standing in his way, ordering his men to shoot their hostages to serve as a distraction, ''cutting off his prison warden's face'' in old security footage -- but the act that solidifies him as a true villain of the Batfamily is indirectly smuggling a signal-broadcasting puzzle box into the Wayne Enterprises building, which leads to the manslaughter of Lucius Fox when the pulse leads a homing missile straight into the side of the building. For no reason other than a sick compulsion to test the Batman's mettle, Riddler manages to hurt over a dozen innocent people, and kill one of Bruce's oldest friends and allies, all in one stroke.
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