Background material explains that Lady Cassandra from Doctor Who had this lifestyle when she was younger and her body wasn't deteriorated. She is a good foil to the doctor's companion Rose Tyler and due to different circumstances, mostly her desire for revenge and getting a younger body, she ends up possessing Rose. The two of them get further contrasted that way. Cassandra basically becomes Rose's Dark Feminine.
River Song was raised to be this - she killed the Doctor with a kiss. Fortunately, it was short-lived.
Victoria Metcalf, the psychotic, poetry-loving bank robber who was the love of Benton Fraser's life in Due South.
Lila in Dexter. Granted, Dexter is most certainly not your typical "moral and upright man", but Lila's willing to go places that even he won't. The nihilistic temptress comes complete with black hair, in contrast to Dexter's blonde good-girl girlfriend Rita.
"Saffron" in Firefly has married numerous men under as many aliases for pretty much the express purpose of ripping them off. Damn good criminal mastermind, too.
Oz. Shirley Bellinger, who drowned her own child in a fake accident based on the Susan Smith case. She gives sexual favours to both inmates and prison guards in exchange for preferential treatment, leading to a Funny Moment when, as she's being led off for execution, Shirley calmly informs Warden Glynn that the guard escorting her has been "coming into my cell every night and fucking me." Faced with a glowering Warden and a prisoner who'll soon be beyond any retaliation, the guard can only mutter, "Bitch."
Ashton Main Huntoon Fenway from North and South (U.S.). She marries James only to gain a sort of political power and wealth despite coming from a Southern aristocratic family, plots against her sweet sister Brett and Billy's romance-turned-marriage, seduces multiple men after a failed attempt to get into Billy's pants...and this is all just Book One...
Vala Mal Doran from Stargate SG-1 was introduced as a straightforward vamp, but she got better.
Princess Archerina in Power Rangers Zeo. Probably only effective on other robots, but she can entrance males via her arrows and make them follow her bidding.
Theresa Conners on the short-lived series EZ Streets, played by Debrah Farentno. She sleeps with her mob boss client as well as the detective tasked with bringing him down.
Tammy, Ron Swanson's ex-wife on Parks and Recreation, played by Megan Mullally. She's the deputy director of the Library department who seduces her ex Ron, the head of Parks and Rec dept., and sleeps with him again in exchange for the empty lot his deputy director Leslie Knope wants to turn into a park. As Tammy tells Leslie: "Les, there are two kinds of women in the world. There are women who work hard and stress out about doing the right thing, and then there are women who are cool."
On her first appearance on Hercules, Xena was this as well as a Dark Action Girl. By her second appearance, all traces of the vamp had disappeared and she was only the Dark Action Girl before being redeemed.
Sarah from Survivors is first seen using her feminine wiles to manipulate a smitten plague survivor, who she promptly leaves to die after he breaks his leg in an accident. She begins working her way through the male members of the main cast from there.
A very similar character appeared in the original version of the series under the name Anne, although she only appears in a handful of episodes.
The Vampire Diaries has Katherine, who uses her charm and seductive wiles in order to get what she wants.
The X-Files: Frohike and Langly saw Suzanne Modeski as The Vamp, and were really pissed off that Byers fell for it. The truth turned out to be a little more complex. She's actually a woman tormented by a shady government organization that wants to use her smarts and destroy her when she wants to expose them.
Professional dominatrix Irene Adler in the "A Scandal in Belgravia" episode of Sherlock is like this towards all her more influential clients (from whom she gains confidential government and MI6-related secrets during the course of "recreational scolding"), as well as the titular hero. It's not a personal vendetta: she is in cahoots with the "consulting criminal Moriarty and plays off of Sherlock's lack of sex knowledge to get him to do whatever she wants. It somewhat backfires in the end, though Sherlock is so impressed by the fact that she managed to fool him for so long that he compliments her - in a manner of speaking - by saving her life.
The notable thing about Irene is that she does, in fact, develop genuine feelings for Sherlock somewhere along the line; however, she doesn't so much as consider affecting a Heel–Face Turn like in a typical example of the 'villainess' who falls for the hero, instead fully intending to see her mission to thwart Sherlock and his brother Mycroft through to the end. This makes her an unusual cross between The Vamp and a Femme Fatale.
This happens often on Merlin (2008). Thus far we've had Morgana, Morgause, Nimueh, Sophia, Catrina, Lamia, and Helen/Mary using feminine wiles at one point or another to manipulate the men-folk.
Regina in Once Upon a Time clearly wants to be this, trying to seduce men to do what she wants while being as sexy as possible. It never works (probably because the men are Genre Savvy enough to know sleeping with an evil witch-queen never ends well), so she falls back to her magic and/or loyal army.
Star Trek: Deep Space Nine has a downplayed example the Female Changeling, the ruthless leader of the Dominion and the public representative of the Founders. She served as an evil mentor to Odo in "The Search," teaching him about his Changeling heritage and helping him hone his shapeshifting skills. However, during the Dominion occupation of Deep Space Nine, she uses linking and sexuality to weaken Odo's resolve. We learn in "Favor the Bold" that her seduction of Odo isn't just about neutralizing an opponent, but about bringing her fellow Changeling home.
Female Changeling (to Weyoun): Neutralize Odo?! Is that why you think I'm here?! Odo is a changeling. Bringing him home, returning him to the Great Link means more to us than the Alpha Quadrant itself. Is that clear?
Lydia TRIES to be this. With anyone. Including Scott. And Allison's dad. While Allison is in the room. Neither of them notices. She does eventually succeed with Scott.
After a childhood of feeling sickly and unattractive due to her epilepsy and the meds she had to take for it, Erica wants as much attention as she can possibly get when the bite makes her healthy and beautiful. It goes to the degree of overcompensation.
Illithyia in Spartacus combines this with Lady Macbeth. Among other things, she uses sex appeal to encourage her husband in military ruthlessness and get a teenage boy to order a gladiator killed.
True Blood: A flashback shows Bill encounter a woman while badly injured. After she patches him up, Bill thanks her before taking his leave. Only for her to throw herself at him since he didn't try to rape/take advantage of her like every other man who ended up in her home. Bill demurs saying he has a wife and child to return to. The woman angrily reveals she's a vampire and turns Bill because If I Can't Have You….
Tony Stonem from Skins would be a Rare Male Example. His whole character revolves around seducing Girls and a Boy for no other reason than boredom.
Babylon 5: Londo describes his wife Mariel as being drawn to men of power like a moth to flame, but in the end, she burns them.
Parodied in I Love Lucy. In one episode, Cousin Ernie visits the Ricardos in New York but likes the city a bit too much and just plain won't leave. Ernie's a sweet, simple, naive countryman whose mother warned him about "wicked city women" who would try to "vamp" him... which Lucy decides to use to her advantage dressing up as one of those "wicked city women" to scare Ernie away. This entails putting on a slinky, sparkly black dress (loaded with about 800 strings of pearls), High-Class Gloves, and a black bob wig, in addition to carrying a long, elegant cigarette holder and walking around with a ridiculously exaggerated sashay (quote Ernie: "You got quite a hitch in yer gitalong!"). When Lucy introduces herself as a city woman ("I'm gonna VAMP you"), Ernie freaks out a bit, until she sashays over and begins "vamping" him... with involves her sashaying in place while repeatedly mussing his hair. After confirming with Lucy that she's vamping him, Ernie tells her he likes it, at which point Lucypanics and runs off, with Ernie in hot pursuit.
The Affair: Alison is pretty upset that she's portrayed this way in Noah's new book, where her character aggressively seduces him, is a drug-dealing sociopath, and is finally run over by Noah's character.
Forever Knight: The bad girl in one episode was a stripper who seduced various men and induced them to commit crimes for her before killing them, pretty much solely for thrills (she was already wealthy enough to live quite comfortably). Even Nick himself (a literal vampire) is tempted.
The very first Monster of the Week, Constance Welch, is one, a ghost who targets unfaithful men, seducing them into being unfaithful (or simply forcing them to be if they resist her) and then killing them.
Lilith, when she's not possessing a little girl. In the episode "The Monster At The End of This Book", she attempts to seduce Sam into making a deal with her - literally, since having sex with her is what will seal the deal.
Ruby is a much more successful example, completely succeeding in her goal to get Sam to kill Lilith, thus bringing about the Apocalypse.
April from the episode "I'm No Angel", who seduces Castiel as part of a plan to torture and murder him.
The Siren from "Sex and Violence", the Vetala from "Adventures in Babysitting" and the Amazons from "The Slice Girls" are all monsters that use sex appeal to lure their prey.
Bela Talbot from season 3.
Played for laughs in The Windsors, where Pippa is insanely jealous of her sister marrying a prince and so uses her good looks and great ass to seduce Prince Harry. However, she's not above dropping him when someone richer comes along.
Motherland: Fort Salem: Scylla is a beautiful young woman from the Spree terrorists who infiltrates Fort Salem, seducing Raelle to get her on their side. However, it gets zigzagged as Scylla then falls for Raelle genuinely, then is unable to go through with bringing her into their grasp.
