Scott Pilgrim Takes Off: In episode 7, it is revealed that in the original timeline, Scott and Ramona honeymooned at Universal Studios Orlando. They are seen in a photograph with a minion and Old Scott mentions meeting Optimus Prime.
In "Any Given Sundance", Principal Skinner mentions that his constant attempts to sell a screenplay got him banned from the Universal Studios Tour. Lisa asks if it's the one in Orlando or Hollywood, and Skinner exclaims "There's one in Hollywood?"
In "The Man Who Came to Be Dinner", one of the ways the Simpsons get to Diz-Nee-Land is via a StarWay-esque escalator system.
In "Halloween of Horror", Bart and Lisa go to Krustyland's "Halloween Horror Night", a parody of Universal's Halloween Horror Nights.
Teen Titans Go!: In the Warner Bros. 100th Anniversary special, the Titans run a tour of the WB studios for their fellow cartoon characters. When they pass by the Friends fountain, Porky Pig asks if this is the part where the shark jumps out at you, and Robin tells him that's a different studio.