Artemis Crock, who shares the name and backstory of her comics counterpart, but becoming the new protege of Green Arrow who is a female archer, also has elements of Arrowette and Mia Dearden thrown in. Notably, the chest emblem on Artemis' costume comes from Mia's Speedy costume. She eventually takes the Tigress name of her original comic counterpart as part of an undercover mole plot, later permanently using the name when her boyfriend Wally West / Kid Flash died. Still, she stayed a hero, while Arrowette appeared anyway.
A seeming Canon Foreigner character named Sphere, an alien sphere brought to Earth from a Boom Tube, turned out to be the Super-Cycle, a living vehicle used by Young Justice in the comics. However, it and the Super-Cycle associated with the Forever People were different characters originally, but are the same character in the show.
Wolf, a mutant wolf who joins Young Justice during a mission in India, takes his visual appearance and his origin as the result of being exposed to a serum from Rex the Wonder Dog (albeit with the serum injected by the Brain and being a mixture of Bane's Venom and the Blockbuster formula), and his role as Conner Kent's animal companion from Krypto the Superdog.
Match is also given elements from various Bizarros (backward S, insanity due to the difficulty of copying Kryptonian DNA, the tie-in comic even started having his skin turn grey), especially S-01 (being a faulty previous attempt to clone Superman that's been secretly stored in a stasis in the bowels of Cadmus), and Superboy-Prime (carving an S-symbol into his chest via heat vision, having black eyes, status as the "original" Superboy).
The Guardian seems to be the second Guardian, the clone of Jim Harper who was the first. However, he's a clone of Jim's grand-nephew Roy, but sports an armored costume more similar to that of Jake Jordan, the Manhattan Guardian from Seven Soldiers.
The Queen Bee in the show is the Post-Crisis version from Justice League International, a dictator who rules Bialya, but also possesses the mind-control abilities of the original Silver Age Queen Bee (although these are changed from hypno-pollen to a pheromone that can control men and LGBT women).
Mark Desmond/Blockbuster gets his role as control-freak Cadmus boss who created Superboy from Director Westfield.
Klarion the Witch Boy's status as an insanely powerful and evil Lord of Chaos comes from The Child, a foe of Hawk and Dove and the JSA. Also, in "Misplaced" he takes the role of Bedlam in JLA: World Without Grown-Ups.
The role of Eugenie Hedstrom from the Flash comic book story "Happy Birthday, Wally!" as the patient who needs a heart transplant that Wally is transporting across the country is handed to Queen Perdita, the teenage queen of Vlatava and a character Greg Weisman created originally for DC Showcase: Green Arrow.
Red Tornado's siblings Red Torpedo and Red Inferno believed themselves human and became superheroes during the Golden Age. Red Torpedo's human identity was the original Red Torpedo from Quality Comics, and Red Inferno was the All-Star Squadron character Firebrand.
The Runaways are based on the made-for-TVmembers of the Superfriends, but also bear some similarities to Lex Luthor's iteration of Infinity, Inc. (a team that later appears in the revival seasons)
Tye Longshadow also takes his surname (and voice actor) from the Justice League expy of Apache Chief.
Static replaces Black Vulcan in the show's group of Superfriends analogues. His jacket, however, is a nod to Vulcan's black and yellow costume. (Presumably, it's because Black Lightning, on whom Black Vulcan was based, is already in the show elsewhere.)
The tie-in comic reveals that the Ultra-Humanite's gorilla body originally belonged to Tolifhar, one of the Gorilla Knights from Gail Simone's Wonder Woman run.
The Reach's interest in the meta-gene and the way their experiments lead not only to new heroes but to Earth's superhumans realizing this is where their powers come from, seems to be borrowed from the Dominators in Invasion!, which made sense given that the season they were involved was titled Young Justice: Invasion.
Jay Garrick (the original Flash) assumes Max Mercury's role as the elderly Golden Age speedster who takes in Bart Allen and becomes his guardian after the boy arrives in the present.
The entity that lives in Halo's body is a Motherbox, taking the role of the Aurakles from the comics. At the same time, Halo themself, a hero who was also the Anti-Life Equation, is based on obscure Young Justice comic member Empress, who had a piece of the Anti-Life Equation in her body.
Lynn Stewart, Black Lightning’s ex-wife, turns out to be the sister of John Stewart, the Green Lantern. This makes her a composite of her comic counterpart and Rose Stewart, John’s deceased little sister from Judd Winick’s Green Lantern run.note Lynn was intended to be John's sister in the comics, hence the name, but the original creator never established it, and nobody else followed up on the idea.
Queen Bee's team of supervillains is a composite of Onslaught (the name of a supervillain team that opposed the Suicide Squad) and the Fearsome Five (Mammoth, Shimmer, and Psimon are members, all of whom are Teen Titans villains), with Infinity, Inc. villain Icicle Jr., Wonder Woman villain Devastation, Black Lightning and Static villain Holocaust, and Canon Foreigner characters the Terror Twins also added.
Dabney Donovan is an alias used by Dubbilex while in disguise, instead of an evil scientist.
Clayface is primarily based on the Matt Hagen version of the character, but the mystical pool that gave him his shapeshifting powers is instead a Lazarus Pit, depicting him as a former member of the League of Shadows. The audio play "The Prize" sees him undergo a Heel–Face Turn similar to the one the Basil Karlo version of Clayface underwent in Batman (Rebirth).
Lady Shiva is an assassin and wears a similar outfit to her Post-Crisis self, but like her Pre-Crisis counterpart, she uses a sword instead of her bare hands. She's also combined with David Cain, Cassandra's father in the comics, as the one to train Cassandra as a living weapon, keeping her from being able to read and speak (this time by slitting her throat as an infant).
Sportsmaster takes some traits from David Cain, being reimagined as a blond assassin who abused his half-Asian daughter and put her through Training from Hell as a child to mold her into the perfect assassin.note By contrast, the comic version of Sportsmaster was a redhead who genuinely loved his daughter and was a standard supervillain rather than a hired killer. It's blatant enough that he borders on outright Expy status.
Orphan replaces The Joker as the one who cripples Barbara Gordon. Joker still appeared, but his involvement is reduced to being a target that Shiva deploys Orphan to kill, only for Barbara to jump in the way of her sword to keep her hands clean from killing, which ensures Orphan turns against her mother.
Mist is a female, like Nash / Mist II, but her name of Andie Murphy is taken from an alias used by Kyle / Mist I, Andy "Murph" Murphy.
Child is given a Gender Flip to become a female, more akin to fellow Lord of Chaos Shivering Jemmy, but retains having Flaw as a minion, like Child in the comics.
Ocean Master retains his role as Aquaman's evil brother, but is later reborn in a clone body of the long-dead sorcerer Arion, who he assumes the identity of.
Lor-Zod, based on his evil portrayals in Superman Family Adventures and Superman (Rebirth) but reinterpreted as a rogue time traveler, takes Goldface's role as the killer of the Green Lantern Tomar-Re. His murder attempt on Conner Kent is also a reference to Superboy-Prime, who killed Conner in battle only for him to be revived by the Legion of Super-Heroes, with Lor targeting Conner specifically to stop the Legion's formation and, therefore, their subsequent defeat of his father General Dru-Zod. His role as a Legion villain, in tandem with his connections to the Phantom Zone, also brings in elements of Drax, a character from the Legion of Super-Heroes cartoon who was a Phantom Zone inmate.
Beast Boy, when discussing why he named his therapy dog Wingman (itself also a reference to his voice actor's dog of the same name), stated it was about the Crimson Avenger's sidekick Wingman, combining his actual sidekick in the comics, Wing, with the name of Wingman, a Batman counterpart from Sweden.
Ursa Zod, the wife of General Zod, becomes a new incarnation of longtime Fatal Five member Emerald Empress. Also verges with Decomposite Character / Legacy Character as the original Emerald Empress is name-dropped as being from the 31st century.
For his services to Apokolips, Ma'alefa'ak is rewarded with settling his fellow White Martians on the planet of Durla, suggesting that Durlans are actually a descendant race of White Martians in this canon rather than a separate race of shapeshifting aliens.
