8-Bit Theater: Red Mage uses this as Black Mage is about to burn the Rat's Tail they need for the class change. He puts on a levitation spell so he can do the slo-mo jump (but applies too much and is left hanging for a while), and does the Big No just to be dramatic.
Tedd's father uses this when he's realizes he's said he'll pay for eight teenagers to eat pizza, while at a meeting far away. The person he's talking to thinks her ideas are being criticized. The comic is helpfully titled 'I Graduated from the George Lucas Directing Academy'.
Dr. Wily does the traditional NOOOOOOOOOOOOOO! When he realises he's in a catch 22 as the VP of Nexus and doing anything to ruin Nexus would cause Blues to torture him, meaning he would have to go against his nature and save it from the stupidity that was President Haggar.
Alex Willams, replaces the Big No with the Big FUCK, and purposely extends it to buy time to think.
He did a simple Big No before. It lasted something like thirteen comics.
Plays... oddly when Gilgamesh Wulfenbach is led to believe that Agatha was killed by a rogue clank. He begins the wind-up to a Big No before cold logic takes over. However, in a flashback to the same scene, Gil is in the classic "Big No" pose. So it's unclear whether he did one or not.
Many Girl Genius characters get a Big No, of course. Gil demonstrates correct form here.
The entire Circus of Adventure lets loose a joint one during the battle with the monster horse beastie when animal-trainer Professor Moonsock gleefully announces she's releasing her poisonous sky-wyrms.
In the language of the Geisterdamennote "ghost women" it sounds NEEEEE!
Scully gets one in Monster of the Week when the Flirty Science Guy gets shot:
Scully: NOOOOO! Whose romantic advances will I politely rebuff now?
Dragon Mangospoofs this when Pumpkin's father returns after a four-year journey into faraway lands and gives Mango a rare treat, called a "sweet swirl stick", which Cherry eats in one bite.
Jason from Something*Positive has a fairly unique one when realizing he's just turned down a 3-way with his wife and hot friend.
Professor Utonium: What's that you got there? Is that a... Wh—Th—P...P.... Buttercup: Parent-Teacher Conference... Professor Utonium:NNNNNOOOOOOOOOOOOOOOOOOOOOOOO!!!
A Nooooo! that bursts out of the panel in Jayden and Crusader.
Alpha Bitch Sam yells this after getting sentenced to community service as punishment for blowing up Piscataway Stadium.
Princess Pi herself says this when the new princess, Gertie, makes her genie, Bottle Blonde, encase her own bottle in concrete, so that Pi could not make Bottle Blonde turn Piscataway back to normal.
In Gunnerkrigg Court, the first time Antimony meets the Psychopomps, she does this when seeing a dying child's memeory of accidentally burning his house.
Trickster Mode Jane: I feel better than alright! I feel great! I feel so alive! I feel so... CAUCASIAN! Jake: NOOOOOOOOOOOOOOOOOOOOOOOOOOOO
Kanaya's response to Rose asking if she was going to break up with her was a frustrated no punctuated by shoutpoles (!).
Terezi gives one punctuated by crying when Karkat points out that Gamzee will likely leave her.
Taking this to increasingly exaggerated levels seems to have become a running gag. John's big No after a Mind-Controlled Jane kills Karkat takes so long he has to pause for breath at the end of each page before continuing, only for him to immediately perk back up when Jane revives Karkat four pages later. Later, Dave's big no is so long that it stretches down to where his apartment building reaches the lava of LOHAC after Grimbark Jade kicks WV over the edge of the building.
In Sandra on the Rocks, Tatiana invokes the short, intense version here when she discovers that something ... terrible ... may have happened to her beloved boss Domenico.
In Knights of Buena Vista, Mary has her Player Character do this when another PC is stabbed (it was only 3 damage, but the character wouldn't know that).
Boy and Dog: When Murphy thinks the vacuum cleaner is a monster but Rowan wants to see it, Murphy says, "Rowan, nooo!".
In Darths & Droidsepisode 1596, Tarkin, who here has a fake French accent (and isn't much of a bad guy and doesn't fancy seeing a city blown up) does a big "Non!"
"Nooooooooooooooooooon!"
In Vampire Girl, Levana has three of these in a row that get bigger than the one before when it's implied that a struggling scuffle in the Vampiress' castle leads to her being biten and turned back into a vampire.
Dean & Nala + Vinny: On a train trip, while Dean sleeps, his cat Nala takes the opportunity to use his laptop to impersonate him in an online Q and A version. Their friend Vinny (a roach) can only watch in horror:
Q: "Have you used your camera lately?"note Real-life Dean had a death in family and spent some time at in Scotland, during which he did not post. The comic incorporated the death into the storyline. A:(typed by Nala) "Not really. Wanna buy it? €20." Vinny: No!
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