- Lucretia of The Adventure Zone: Balance gets one of these. Her theme appears four times, all during moments that are essential to her character and the story. "Madam Director" plays during The Crystal Kingdom and is reprised during The Stolen Century. Her leitmotif appears again as "Lucretia" in episode 66 and reprised for a final time in the finale as "Lucretia (Reprise)".
- In early episodes of Atop the Fourth Wall, the arrival of '90s Kid would be marked with "Smells Like Nirvana" and it would often keep playing until he was cut away from. Later, it was swapped out for the genuine article and is usually not used at all if he's appearing in a dramatic scene.
- The entity Missingno. has "evil" or alternatively "satanic" from The Ren & Stimpy Show soundtrack (though that bit was unintentional.)
- In Dr. Horrible's Sing-Along Blog, the eponymous character has his own theme which plays during the act openings and as part of two songs. In one song, when he switches from philosophizing to Evil Gloating halfway through, the background music also changes to his theme repeating until the song's end. In another song, a slowed-down version with heavy downbeats plays during a Suit Up of Destiny sequence.
- In Echo Chamber, Shannon's entrances are marked by "Psycho" Strings.
- The title character of 80's Dan has "Take on Me".
- It's Gendo!
- In Everyman HYBRID, HABIT has "Who Could Win a Rabbit" by Animal Collective. And it's just as scary as he is.
- In Farce of the Three Kingdoms, Cao Cao's leitmotif is Yakety Sax. Everybody can hear it, too. It only plays when appropriate, luckily.
- The utterly deranged version of Fluttershy depicted in Friendship is Witchcraft seems to be associated with "Hellfire".
- In the Gorgeous Freeman videos by Antoine Delak, Dr. Breen has two the first is Dr. Evil's Theme and the Pornosonic song "A Happy Ass: Special Delivery".
- AngelDust from Hazbin Hotel has a song called ''The Slutty spider Polka Pop'' by Gooseworx Music
- Hero House has Kratos' theme, which plays whenever he's onscreen.
- Kung-Tai Ted and The Ninja-Style Dancer (From Atop the Fourth Wall, above) seem to share "Jet-Set" by Alphaville.
- Nearly every character and concept in Nightmare Time has their own leitmotif:
- "Paul's Theme," the opening bars of "Inevitable" from The Guy Who Didn't Like Musicals, has been retooled into a theme for spookier moments in general, especially concerning the Witchwood.
- The instrumental from "Show Stoppin' Number" is used to represent Professor Hidgens, and plays when he tries to pitch his musical.
- "Show Me Your Hands" is used to represent Sam Sweetly, and the Hatchetfield police in general.
- "Cup of Roasted Coffee" is used as Emma's leitmotif. It gets a minor key Dark Reprise in the barfight scene in "Forever and Always," with the "Hey Mr. Business" melody playing rapidly as Emma kills the bar patrons.
- The title song of "Forever and Always" is used as Paul and Emma's Love Theme.
- Similarly, "Take Me Back" is Tom and Becky's Love Theme.
- "What Tim Wants" is played during heartfelt moments between Tom and his son, Tim.
- Tinky has his own sort of theme, a rush of discordant notes.
- The opening notes of "Time Bastard" are used as the theme for the Bastard's box
- "What if Tomorrow Comes" is used when Hannah uses her powers, or when the overarching cosmic themes of the story are discussed.
- Miss Holloway has a leitmotif that plays when she uses her powers or is discussed, a slow, dissonant, meandering theme.
- A lighter, bouncier version of "CaliforM.I.A." is used for the more lighthearted scenes between the Foster family.
- "Our Doors Are Open" is used for scenes involving Frank Pricely and Toy Zone.
- The tune that plays most over The Nostalgia Critic's depressed moments is this crushing piece of Lonely Violin Music.
- As of his comeback, the character himself seems to have an instrumental version of "the Review Must go On" as his theme.
- Shorts Wars: Attack of the Killer Queen from Deltarune frequently plays in Danno's shorts. It gets more and more distorted while Clone Riggy is in place of Riggy. Before Riggy was cloned, however, it was frequently replaced with Pandora Palace, which has the same melody.
- Whenever Sting showed up in Dune (1984) wearing a speedo, The Nostalgia Chick would play "Don't Stand So Close To Me".
- The four main characters of The Platoon of Power Squadron each have one that plays whenever they use their superpowers. They can be heard here.
- Any appearance by Tex in Red vs. Blue is usually accompanied by the opening bars of her theme.
- With the exception of Season 8, though her new theme, Agent Tex contains a nod to her original leitmotif.
- Two villains had those, The Meta with "(When) Your Middle Name Is Danger" and Locus with "Loom".
- Carolina doesn't officially have her own, but when she's fighting, expect some variant of Extraction (starting at about 2:08). The tune has been played differently numerous times depending on the setting, including with guitars near the end of Spiral, twice in Mental Meta Metal, and with a particularly heart-wrenching piano in Ballet Breakup.
- The Hitchhiker/Malachite from Suburban Knights has a menacing, drum-based one that plays whenever he appears.
- All the videos of French-Italian YouTuber Tess Masazza open and close with the French band Capashen's song Secret In Their Eyes.
- In the The Thrilling Adventure Hour, Nightmares the Clown's appearances are heralded by a creepy circus tune, followed by Sadie Doyle happily shouting "Clown!"
- Frequent True Capitalist Radio caller 213 (the Ghetto Capitalist) is often accompanied by the sound of a crying babynote as he trolls the the host Ghost. This often leads to Ghost telling him to "stop choking that damn kid".
- Grand Dad from Vinesauce has the 8-bit instrumental remix of the theme song from The Flintstones thanks to SiIvaGunner.
- Welcome to Night Vale's theme song, "The Ballad of Fiedler and Mundt", could be considered this for Cecil. Its sudden appearance in Episode 48 signals his return.
- When Nash of What the Fuck Is Wrong with You? encounters rampant douchebaggery on a massive scale (summarily titled 'Douchequakes') he plays Gunther's Ding-Dong Song over clips of stuff collapsing, falling over, et cetera.
- One episode of WTFIWWY Live! was so bad, he played the song over the live feed of the webcast.
- In The With Voices Project, Mr. Monokuma’s Lesson from Danganronpa becomes the leitmotif for King mogeko.
- The Yogscast has "I'm a dwarf and I'm digging a hole! DIGGY DIGGY HOLE!" and variations of such. It comes from Shadow of Israphel, when Simon Lane is digging for K. Peculier's mining post.
- In Yu-Gi-Oh! The Abridged Series, the opening for Sexy Back loops whenever Duke Devlin speaks. Also, the "Double Fine" riff of Judas Priest's Hellion plays at the beginning of any of Dartz's scenes.
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