It's basically ending a sentence with a comma and "Bitch!", as a way of someone verbally making an exclamation point in dialog.
It did get overused, so now it's a Discredited Trope. You're as likely as not to see it thrown in a sentence just to be funny, even though there is no other reason to emphasize a point this way. Bitch.
Since talking this way is inherently humorous, actual dramatic scenes are more likely to use "son of a bitch" (if the target is male) or some other insult (if the target is female) instead.
This can overlap with Quote Swear Unquote, Obligatory Swearing.
Compare (but would not overlap with) Verbal Tic, Precision F-Strike.
Scotch: Chip… you son of a… BITCH; you… you know damn well that you… you and your family aren't… welcome here… after what… what happened long… long ago so why the… hell did you… you come here to… N-N-N-N-N-Novi Finis City anyway?
From Michael to Dwight on The Office: "Hug it out, bitch."
On Saturday Night Live, any conversation Tracy Morgan holds with Lorne Michaels is likely to end with the former demanding, "Get me a soda, bitch!"
Dave, a contestant on season one of reality cooking show Top Chef, reached the height of reality TV popularity after telling another contestant Tiffani "I'm not your bitch, bitch!" in response to endless haranguing by the latter.
X-Play: Morgan Webb's tagline on a commercial about her visiting conventions in Japan: "Konichiwa, bitches."
X-Play's coverage of G-Phoria one year featured Ratty sneaking into the awards by jumping off a nearby building. This action was accompanied by "Geronimo, bitches!"
Another from Ted when he wanted to comfort Robin and she didn't want any...
Robin: Well, then, you're fired.
Ted: You can't fire me. I'm union, bitch!
As well as Lily's "You just made the list, bitch!"
How about Barney speaking French? "Parce que j'ai le jeu, mes chiennes. Translation? Cos I got game, bitches!"
That doesn't quite meanwhat he wanted. "Chiennes" literally means "female dogs." If he wanted "bitches" in the slang sense, he should have used "salopes" or "garces" depending on personal preference.
Wanda Syke's "I'ma Be Me" had her saying "Yeaaah... Obama, bitch!"
Modern Family: "See, we're all going to get past this and be friends again, and this was all set up by me, Mitchell, who is not a mummy's boy but is instead a insightful and considerate person, so make a note bitches!" in episode 4.
Elizabeth once disguised herself as Amelia and walked into Amelia's house yelling: "I'm your worst nightmare! I'm a motherfucking crossdressing lesbian Jew, BITCH!!" in That's Just Me. However, is most versions of this episode, the line is censored, changing it into: "I'm your worst nightmare, because I'm a crossdressing tomboy, man!"
Okay, so they'll censor that, but NOT any of the characters' foul mouths, all of the fanservice/nudity scenes, OR any of the mental illness jokes?
They also joke a lot about suicide, even though the main character almost committed it in an earlier episode.
Perhaps they censored it because Elizabeth ISN'T religious? The episode was called 'I Don't Wanna Cut My Hair! What Happens When Elizabeth Crossdresses' and it was in the 6th season.
From Spartacus: War of the Damned, when warrior-woman Saxa is confronted by two large gladiators who want to kill some prisoners entrusted to her by Gannicus...
Donar: "I do not see Gannicus among you. No cause to heed his bitch." *Attacks. Ends up with Saxa's knife at his throat and his buddy on the ground.*
Saxa: "I give cause, bitch."
In season 1 of Top Chef, Dave told Tiffany after Restaurant Wars "I'm not your bitch, bitch!"
The WNYC program Radio Lab episode Choice(501) contained an instance of this, subverting the line tagged onto the end of many radio broadcast introductions. "Stay with us...bitches."
"BIIIIIIIIITCH!" is the calling card of Mike Clark, of WRIF-Detroit's drive-time radio show Drew and Mike in the Morning. Callers to the show frequently request Mike "send out a BIIIIIIITCH to the folks working at (insert company name here)."
One of Detroit radio personalities Dave & Chuck the Freak's catch phrases is "It's Friday, Bitches!" Actually, it's more than just a catch phrase - there's a short rap song with the title that was made just for them (which is played on the Friday shows, naturally), merchandise featuring the phrase, and even an annual event called the Friday Bitches Bash.
Stand-Up Comedy, Bitch!
Inverted by David Cross, on Bigger and Blackerer: He details a game to play with friends at a restaurant. When talking to the waitress, make the first thing you say be "Yeah, bitch," But in as low-key a manner as possible. Then, be as polite as possible when making your order. The object is to make it so that the waitress won't be sure you called her 'bitch', but would feel uncomfortable asking such a polite person if he did.
"By the way, if you ever have a job where you have to give speeches in front of people, pepper in the phrase 'suck it.' Very empowering. Just be like 'As you can see from our Powerpoint presentation suck it...' And your clients will be like, 'Did he... did he... did he say suck it? I like this guy... he's a go-getter.'"
True Crime: Streets of LA. uses this liberally, but I guess the greatest example is when Nick Kang, the protagonist, finishes off the crime lord who had his father killed over a money laundering scheme. While this could be a bit of a solemn moment, as he watches his father's killer burn to death in a plane crash, he follows it up by saying: "Try Laundering THAT, bitch!"
Duke Nukem: Duke, despite his extreme womanizing, is a Chivalrous Pervert who never insults the objects of his desire. When you hear him confront the female monster Alien Queen and bellow, "I'm gonna kick your ass, bitch!", you know it is on.
Left 4 Dead's Crash Course DLC has hopeless optimist Louis, of all people, scream "Oh, it's a bitch apocalypse now, bitch!"
Borderlands plays this straight with Roland, the Soldier. Upon scoring a kill with a Critical Hit, he may shout, "Critical, bitch!", or "Critical, biatch!".
Marcus: (after stabbing Myrrah with Dom's knife) You feel that? That's from Dom...and everyone else you killed you bitch!
Batman: Arkham City falls into this a few too many times. You will hardly ever hear any other curse word used, leading to several grunts using "bitch" so many times you'd wish they'd just say something else. For major characters, Two Face takes the cake, especially when he's going against Catwoman. Makes you wonder if he knows any other insults.
Tankmen 2: "You should've used the Pythagorean theorem, bitch."
The end of Neurotically Yours' "Caffeine Conspiracy" has Franklin the Star Shmucks peddler warn his superiors over walkie talkie that "The squirrel is on to us." A moment later, he starts another transmission to add "bitch."
Dinosaur Comics had a strip about the overuse of the word "bitches", and suggested alternatives like "fellows", "gravy", and "Monterey Jack". Possibly a Lampshade Hanging, since T-Rex is known for exclamations like "Phlogiston, bitches!" and "Live and learn, bitches!"
Most of the guest characters of The Angry Video Game Nerd ("Or do ya wanna play my game, bitch?"). And sometimes the Nerd himself ("Yeah, Super Mecha Death Christ 2,000 B.C. Version 4.0 Beta, bitch.").
Cracked.com has claimed at least once to "own the Internet, bitch."
Read a Book "It's called Speed Stick, bitch! It's not expensive, bitch!"
slowbeef: I— maybe it was, like, how to get a refund... Like, "Don't be afraid to take back your pumpkins." Diabetus: This is an example of a defective product. Here's what you should do in this situation, bitch.
In a Cutaway Gag in Family Guy, the MGM lion cub gets laughed at by his classmates when he wants to roar at an audience when he grows up. Soon, the lion is grown up and shouts "What's up now, bitches?!"
After carefully outlining the many, many reasons that global warming is real, a scientist famously ended her presentation with:
Richard Dawkins, at the end of a debate with an anti-scientific creationist gives us this gem: "If you base medicine on science, you cure people. If you base the design of planes on science, they fly. If you base the design of rockets on science, they reach the moon. It works...bitches." Also doubles as Sophisticated as Hell.