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In The Stalking Zuko Series, Katara says this word for word in her narration when Sokka plans to introduce a sixth flavor into five flavor soup... fireflakes. For context, Katara has, so far, gone from absolutely loathing fire flakes to slight acceptance of them, not unlike her relationship with Zuko.
You can't do that to five flavor soup.
You just can't.
Films — Animated
Scar from The Lion King: He wants Zazu to sing him something happy sounding instead of the downtrodden depressing songs he's been singing him.
Scar: Come now, Zazu, sing something happy, with a little...Bounce!
Zazu: <deadpan>Iiiit's a Small World Aaaafter Aaaaall
Scar: No, NO! ANYthing but that.
Films — Live-Action
Lawrence's reaction to hearing the phrase "a case of the Mondays" in Office Space.
Peter: When you come in to work on Monday and you're not feeling real well, does anyone ever say to you "Sounds like someone has a case of the Mondays"?
Lawrence: No... (disgusted) No, man. Shit, no man! I believe you'd get your ass kicked saying something like that, man.
While shooting Dawn of the Dead, a pregnant extra approached George A. Romero and Tom Savini to suggest a shot where while being killed, the zombies rip her baby out of her womb. Romero and Savini immediately responded with this trope.
Ironically, the 2004 remake included a zombie infant clawing its way out of its mother after she succumbs to a zombie bite.
In Men In Black (the sequel) at the end the agents try and console Agent J with unwanted advice and well-wishing. At the same time Zed begins aluding to freaky kinky sex adventures of his own leading to Agent J saying "You, no. You, No! and YOU (Zed)... HELL no!!"
The phrase even made it onto a t-shirt, with the profits from shirt sales going to the Helen Bamber Foundation, an organization that helps women who have been forced into prostitution...which happens quite a bit in the books of the series, by the titular character and the bad guys alike.
In the Warrior Cats series, Lionblaze thinks about his Clanmates and wonders if any of them might be siding with the Dark Forest. When he gets to Whitewing, his thought is "Never. Just.... never."
The phrase "Don't. Just...don't," is said by the Doctor whenever any of his companions try to put on an accent or speak in another style. So far, he has said it to Rose, Martha and Donna. It's given an incredibly dark reprise in "Midnight" after a traumatizing encounter with a voice-stealer.
At the climax of "Journey's End", the Doctor has more than a half-dozen companions in the TARDIS to help him tow planet Earth back home. He happily dashes around the console room giving instructions to Rose, Mickey, Martha, Jack, Donna, and Sarah-Jane. "Now we can fly this thing - (n-no, Jackie, no, no, not you, don't touch anything, just...stand back) - like it was meant to be flown!"
In Scrubs, Turk says this when JD tries to call him Black Whale.
Said in iCarly about what to do if Kevin asks if you want to see his "onion ring".
This is strongly implied to be the reaction to Sheldon's suggestion of moving Jerusalem to Mexico to eliminate the conflicts between the Hebrews and the Palestinians in The Big Bang Theory. When first proposing it to the Dean, the Dean politely excuses himself with the North Korean exchange student, only to privately tell the student that Sheldon is insane. Later, when telling it to a Jewish professor, the professor tells him to go away, and when Sheldon suggests the idea to Howard Wolowitz, all Howard can do is give an aghast and silent reaction to Sheldon before he eventually nixes the idea.
Robin: No. No, no, no, no, no, no, no, no, no, no, no, no, no. Barney and I are not together. No...no.
Barney: Really? Sixteen noes? Really?
On Parks and Recreation, Ron's girlfriend Diane drops her daughters off at the office for Ron to babysit. Jerry tries to chat with them and gets this reaction. The sad/hilarious thing is that he's not even a little surprised.
On The West Wing, this is how Leo reacts to Bartlet's idea to bring in Cloud Cuckoolander Lord John Marbury as an expert adviser on the India/Pakistan conflict. Bartlet doesn't even say his name- he just looks at Leo and raises his eyebrows. When we finally meet Marbury, it's pretty clear why Leo reacted this way- especially because Marbury has a particularly weird relationship with Leo.
In the third Blackadder, Blackadder shouts "No, NO!" when Prince George starts describing what it's like to be "radished" for non-payment of debt at the Naughty Hellfire Club.
In the iOS game Highborn, when a wizard suggests that Jessica the Gorgon might be pregnant, Trillian's reaction is "No! Don't you even suggest THAT!"
When Shepard decides that Maya Brooks should be the one to go through the vents to sneak into Khan's panic room, she says "What? NOO!! What?!"
During the party at Shepards apartment, you may find EDI questioning a thoroughly flustered and embarrassed Samantha Traynor on how she found EDI's voice sexually attractive when they first met. This eventually moves on to EDI offering to send her links to lesbian organic-on-synthetic porn sites, to which Traynor responds "What? NO! No, no, no, no, NO! Okay, sure." Tali's Squick reaction completes the scene.
In SCP Foundation, the response of SCP-076 (Abel) at being given the mission to approach SCP-001 (Gate Guardian), apparently an angel (more specifically: One of the Cherubim set by God to watch over the entrance to the Garden of Eden). This answer was given before what the mission actually involved was revealed.
Also said word for word in response to 507's request to challenge 076 to fifty rounds of Tic-Tac-Toe.
In the Strong Bad Email "animal", Strong Bad tries to think up an imaginary animal version of himself, and one of his attempts is a gastropod-like creature called the "red-steckled elbermung". His response to a picture of the elbermung is "No, no, no!"
Porn Critic: Uh..hello...umm (consults sheet)...Sursum? I'm the Porn Critic!
Sursum Ursa: Ahhhh...
Porn Critic: Oh come on, I'll ease you in gently. I have lube!
Sursum Ursa: OK, one: it's Ursa. And two? No. Just...no.
RWBY: In Painting the Town..., right after Roman escapes with the help of Neopolitan's illusory powers, Weiss makes a pun referring to how Yang literally shattered the illusions. Yang answers with this trope word for word, which is ironic since she herself is a Pungeon Master.
The Cartoon Show: Tom's reaction to a possible sleepover with Cartoon Guy is to go Laughing Mad, cut the laughing off with a "nope!", and openly takes his chances with the zombies outside.
During the Yogscast 2013 charity livestreams, Zoey Proasheck reads out fanfic of Ravs and Teep (a giant, sapient dinosaur) making out "for like ten hours". Ravs is not exactly pleased to find out this sort of stuff exists.
My Little Pony: Friendship Is Magic's Equestria Girls has the Human Cutie Mark Crusaders checking how many hits their video made. Human Cheerilee has this reaction verbatim and tells them that "the school computers are for research purposes only".
King of the Hill: Hank reacts this way to Bill's clothing, the uniform of the local baseball league.
The Simpsons did it in Season Eleven's "Beyond Blunderdome", featuring Mel GibsonAs Himself. After Homer is the only person that criticises Gibson's newest movie (a remake of Mr. Smith Goes to Washington) in a test screening, Gibson, himself unhappy with the finished product, recruits Homer to make an edit that will appeal to the common man. Homer's suggestions are... not what Gibson was hoping for.
Homer: Okay, here you need a musical montage where you try on lots of funny hats. It'll let us see your playful side!
Homer: Yeah bu—
Gibson: Just no!
Family Guy: This is David Letterman's reaction when Peter Griffin, disguising himself as James Woods, reveals that his new (and completely tasteless) comedy movie was "September 11th, Two-Thousand-FUN." The public reactions are also exactly as one expects. Which was exactly as planned, as Peter was attempting to ruin James Woods as revenge for Woods stealing his identity.